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Establishing method of type 2 diabetes mellitus rat model and preparation method of high fat diet of type 2 diabetes mellitus rat model

A high-fat feed and method-building technology, applied in the field of medical research, can solve problems such as large performance gaps and large model differences, and achieve the effects of stable blood sugar, low cost, and high success rate of modeling

Inactive Publication Date: 2017-01-11
SHANGHAI PHARMA DONGYING JIANGSU PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The results of literature research show that the existing animal models of diabetes mostly simulate the arterial lesions, neuropathy or infection of diabetic foot. Due to the different research purposes, the establishment methods of the models are various, and the differences between the models are quite large. There is a large gap in actual performance

Method used

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  • Establishing method of type 2 diabetes mellitus rat model and preparation method of high fat diet of type 2 diabetes mellitus rat model
  • Establishing method of type 2 diabetes mellitus rat model and preparation method of high fat diet of type 2 diabetes mellitus rat model
  • Establishing method of type 2 diabetes mellitus rat model and preparation method of high fat diet of type 2 diabetes mellitus rat model

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Experimental program
Comparison scheme
Effect test

Embodiment

[0031] A method for establishing a type II diabetes mouse model, comprising the steps of:

[0032] (1) Cultivation of experimental mice:

[0033] 56 SD male rats, SPF grade, weighing 200-250g, 8 weeks old, all rats were kept in an environment with a temperature of 22±1°C, a humidity of 50±10%, and 12 hours of day and night;

[0034] (2) Model establishment:

[0035] 56 SD male rats were randomly divided into two groups after one week of adaptation in the animal laboratory: 8 rats in group A and 48 rats in group B; group A was given normal mouse feed every day, and group B was given high-fat feed every day for 8 weeks Afterwards, fasting for 12 hours, but without water, weighing, group B intraperitoneally injected STZ solution dissolved in citrate buffer at a dose of 25 mg / kg, the pH value of which was 4.2–4.5, and group A intraperitoneally injected the same amount of citrate buffer One week later, after fasting for 12 hours, blood was taken from the tail vein to measure the ...

specific Embodiment 1

[0039] A high-fat feed according to the establishment method of the described type Ⅱ diabetes mouse model, comprising the following components:

[0040]

[0041] The common feed is calculated by weight ratio: 30% of corn, 46% of bran, 3% of silkworm chrysalis, 13% of soybean, 3% of white flour, and 5% of fish meal.

[0042] A preparation method according to the described high-fat feed, comprising the steps of:

[0043] (1) Weigh 600g of mouse basic feed and 10g of peanuts, mix well, add Huangcheng HC-2000 pulverizer, 38000rpm / min, pulverize for 20min;

[0044] (2) Place basic feed and peanut powder in a stainless steel basin, add 500ml of distilled water, and mix well;

[0045] (3) Add 0.1g of sucrose, 10g of lard, 2g of sesame oil and 4g of eggs to the stainless steel basin in turn, and mix well;

[0046] (4) finalize the shape, adopt the RX-120 small feed processing granulator to press the above-mentioned mixture into a cylindrical shape: length 5cm, diameter 2cm;

[0...

specific Embodiment 2

[0049] A high-fat feed according to the establishment method of the described type Ⅱ diabetes mouse model, comprising the following components:

[0050]

[0051]

[0052] The common feed is calculated by weight ratio: 30% of corn, 46% of bran, 3% of silkworm chrysalis, 13% of soybean, 3% of white flour, and 5% of fish meal.

[0053] A preparation method according to the described high-fat feed, comprising the steps of:

[0054] (1) Weigh 700g of mouse basic feed and 15g of peanuts, mix well, add Huangcheng HC-2000 pulverizer, 38000rpm / min, pulverize for 20min;

[0055] (2) Place basic feed and peanut powder in a stainless steel basin, add 500ml of distilled water, and mix well;

[0056] (3) Add 0.5g of sucrose, 15g of lard, 4g of sesame oil and 8g of eggs to the stainless steel basin in turn, and mix well;

[0057] (4) finalize the shape, adopt the RX-120 small feed processing granulator to press the above-mentioned mixture into a cylindrical shape: length 5cm, diamete...

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Abstract

The invention discloses an establishing method of a type 2 diabetes mellitus rat model and a preparation method of a high fat diet of the type 2 diabetes mellitus rat model. The establishing method comprises the following steps: culturing an experiment rat; establishing a model; observing rat pancreatic beta-cells through an electronic microscope. An experiment result shows that in group A blank rat pancreatic cells, the quantity of pancreatic secretion granules is relatively great and the pancreatic secretion granules are uniformly distributed around cell nucleuses; in pancreatic cells of group B diabetes mellitus rats, the quantity of the pancreatic secretion granules is remarkably reduced and a lot of the pancreatic secretion granules are vacuolized. The establishing method of the type 2 diabetes mellitus rat model is simple in manufacturing, low in cost, high in modeling success rate and low in rat death rate; after modeling succeeds, the blood glucose is stable, and the type 2 diabetes mellitus rat model has very important meaning on researches of type 2 diabetes mellitus.

Description

technical field [0001] The invention belongs to the field of medical research, and in particular relates to a method for establishing a type II diabetes mouse model and a method for preparing high-fat feed. Background technique [0002] In the prior art, in the animal model of inducing hyperglycemia, animals, usually mice or rats, or rabbits, dogs, and monkeys can also be used to prepare STZ aqueous solutions with different concentrations or single concentrations, and inject them intravenously or intraperitoneally. Repeated administration one or more times. Check whether mold is formed after different processing times. Before STZ injection and at different times after administration (generally 2-6 months, 1 year), blood glucose level measurement, glucose tolerance test and serum insulin or C-peptide detection were performed at different frequencies. Weight changes and clinical symptoms. [0003] With the sharp increase in the incidence of diabetes in my country, the numbe...

Claims

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Application Information

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IPC IPC(8): A01K67/02A23K50/50A23K20/163A23K20/158A23K10/30A23K10/20
CPCA01K67/02
Inventor 章少在宋宇
Owner SHANGHAI PHARMA DONGYING JIANGSU PHARMA CO LTD