A colorimetric sensor containing benzothiazole base dye, its preparation method and its application in detection
A technology of benzothiazole base and colorimetric sensor, which is applied in color/spectral characteristic measurement, analysis through chemical reaction of materials, instruments, etc., which can solve the problem of large amount of dye usage, difficult dye, inconvenient carrying and use detection and other problems, to achieve the effect of long detection cycle and high operation requirements
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Embodiment 1
[0028] According to a kind of colorimetric sensor containing probendiazole base dye of scheme in the present invention:
[0029] Such as figure 1 As shown, including polyacrylamide film (PP), polyacrylic acid (PAA), benzothiazole base dye (DB) and chitosan chain (CS), one end of the polyacrylic acid (PAA) is bonded to polyacrylamide On the film (PP), the other end is connected with the benzothiazole base dye (DB) by van der Waals force and physical adsorption and other forces to form a PP-PAA-DB structure; the chitosan chain (CS) and polyacrylic acid (PAA) is electrostatically bonded to further embed the benzothiazole base dye (DB) to form a PP-PAA-DB-CS structure.
Embodiment 2
[0031] According to the scheme described in the present invention, a kind of preparation method of the colorimetric sensor containing probendiazole base dye is as follows:
[0032] Such as figure 1 as shown,
[0033] (1) Preparation of PP-PAA board: take a surface area of 36cm 2 A square PP plate, weighed and placed in 1.00mL of 50% AA / H by volume 2 In the petri dish of O solution, nitrogen gas was blown into the petri dish for 5 min and sealed with a parafilm. It was then placed under a UV lamp for overnight irradiation. Then take out the PP-PAA plate and wash it with NaOH aqueous solution with a pH value of 8 to remove the PAA monomer. Finally, wash it again with distilled water and dry it under vacuum;
[0034] (2) Preparation of PP-PAA-DB-CS board: take 9cm 2PP-PAA plate, place it in 30.0mL pH5.0 concentration of 1×10 -4 mol / L dye solution, let stand at room temperature for 4h. Chitosan with the same molar number as polyacrylic acid was placed in 2% volume acetic...
Embodiment 3
[0037] According to the scheme described in the present invention, the application of a colorimetric sensor containing probendiazole base dye in detection is as follows:
[0038] Such as figure 2 as shown,
[0039] (1) Pre-treatment: Use pumps or plastic bags to collect volatile amine gas substances in food or the environment, and store them in glass containers;
[0040] (2) Color observation of the sensor before reaction: observe with naked eyes or record the color of the pre-prepared colorimetric sensor with a camera;
[0041] (3) Sensor reaction: put the pre-prepared colorimetric sensor into the container containing the sample to be tested, and let it stand for 5 minutes to fully react;
[0042] (4) Observation of the color of the sensor after the reaction: observe with the naked eye or record the color of the sensor after the reaction with a camera;
[0043] (5) Color difference of the sensor before and after the reaction: compare the color difference before and after ...
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