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Method for extracting madecassic acid from Centella asiatica

A technology of madecassic acid and centella asiatica, which is applied in the fields of steroids and organic chemistry, and can solve the problems of large loss, tediousness and low yield of target components

Active Publication Date: 2018-01-26
桂林益天成生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above method is not only cumbersome, the organic solvent is not easy to separate, the loss of the target component is large, the yield is low, and the purity of the extracted madecassic acid is also small

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1) Crush Centella asiatica, add 60% ethanol solution 10 times the total weight of Centella asiatica, and reflux extract once, each time for 1 hour, filter the extract, recover the solvent from the filtrate, add 40°C water to dissolve, filter, and filter the residue Dissolve in 50v% ethanol solution to obtain crude extract;

[0021] 2) Adding 0.1% by weight of lanthanum chloride to the crude extract and mixing, stirring evenly to obtain a mixed solution;

[0022] 3) Put the mixed solution on the X-5 type macroporous resin column, the column speed is 0.5BV / h, first wash with water until colorless, and then elute with 20v% ethanol at a volume concentration of 2BV / h, thin Layer chromatography tracking detection, collecting ethanol eluate containing asiatic acid; then eluting with ethanol with a volume concentration of 40%, the elution rate is 2BV / h, thin layer chromatography tracking detection, collecting hydroxy asiatic acid The ethanol eluent, the madecassic acid eluate ...

Embodiment 2

[0025] 1) Pulverize Centella asiatica, add 70% ethanol solution 20 times the total weight of Centella asiatica, and reflux extract 3 times, each time for 3 hours, combine the extracts, filter, recover the solvent from the filtrate, add 50 ° C water to dissolve, filter, Dissolving the filter residue in 60v% ethanol solution to obtain a crude extract;

[0026] 2) Add 0.5% of its weight of cerium sulfate to the crude extract and mix, stir well to obtain the mixed solution;

[0027] 3) Put the mixed solution on the X-5 type macroporous resin column, the column loading speed is 0.8BV / h, first wash with water until colorless, then elute with volume concentration of 30v% ethanol, the elution speed is 2.5BV / h, Thin-layer chromatography tracking detection, collecting the ethanol eluate containing asiatic acid; then eluting with ethanol with a volume concentration of 50%, the elution rate is 3BV / h, thin-layer chromatography tracking detection, collecting the ethanol containing hydroxy ...

Embodiment 3

[0030] 1) Pulverize Centella asiatica, add 65% ethanol solution 15 times the total weight of Centella asiatica, and reflux extract twice, each time for 2 hours, combine the extracts, filter, recover the solvent from the filtrate, add 45 ° C water to dissolve, filter, Dissolving the filter residue in 55v% ethanol solution to obtain a crude extract;

[0031] 2) Adding 0.3% by weight of praseodymium nitrate to the crude extract and mixing, stirring evenly to obtain a mixed solution;

[0032] 3) Put the mixed solution on the X-5 type macroporous resin column, the column loading speed is 0.6BV / h, wash with water until colorless, and then elute with ethanol with a volume concentration of 25v%, the elution speed is 2.2BV / h, Thin-layer chromatography tracking detection, collecting ethanol eluate containing asiatic acid; then eluting with ethanol with a volume concentration of 45%, the elution speed is 2.5BV / h, thin-layer chromatography tracking detection, collecting hydroxyl product ...

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Abstract

The invention discloses a method for extracting madecassic acid from herba centellae. The method includes the following steps of firstly, smashing herba centellae, conducting reflux extraction through an ethyl alcohol solution with volume concentration of 60-70 v%, filtering an extraction solution, recovering solvent from filtrate, adding water at 40-50 DEG C for dissolving and filtering, and dissolving filter residues in an ethyl alcohol solution with volume concentration of 50-60 v% to obtain a crude extraction solution; secondly, adding rare-earth salts to the crude extraction solution to be mixed and evenly stirred to obtain mixed liquid; thirdly, applying the mixed liquid to an X-5 type macroporous resin column, conducting water washing till being colorless, conducting eluting through ethyl alcohol with volume concentration of 20-30 v%, conducting thin layer chromatography tracking and detecting, collecting ethyl alcohol eluent containing asiatic acid, conducting eluting through ethyl alcohol with volume concentration of 40-50 %, conducting thin layer chromatography tracking and detecting, collecting ethyl alcohol eluent containing madecassic acid, recovering ethyl alcohol, and conducting drying to obtain madecassic acid. The extracted madecassic acid is high in purity and high in yield.

Description

technical field [0001] The invention belongs to the technical field of biological extraction, and in particular relates to a method for extracting madecassic acid from centella asiatica. Background technique [0002] Centella asiatica (L.) Urban is a Centella asiatica tree plant in the Umbelliferae family. Because its leaves resemble a horseshoe or half a copper coin, it is also known as horseshoe grass, even money grass, etc., and some people call it Peppermint, Dimograss, etc. Centella asiatica has a long history of application in the field of traditional medicine in many countries and regions. The internal and external use of Centella asiatica in traditional Chinese medicine in my country has a history of two thousand years, and it was first recorded in the book "Shen Nong's Herbal Classic". According to the "Compendium of Materia Medica", Centella asiatica tastes bitter, pungent, cold in nature, non-toxic, and returns to the liver, spleen, kidney, and stomach meridians....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J63/00
CPCC07J63/00
Inventor 谢冬养黄健军陆美珍叶出良
Owner 桂林益天成生物科技有限公司
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