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Bilayer active medium material and preparation method and application method thereof

A technology of medium materials and active materials, applied in chemical instruments and methods, water/sludge/sewage treatment, biological water/sewage treatment, etc., to achieve high oxygen release rate, prolonged oxygen release time, and low oxygen release rate

Active Publication Date: 2017-01-25
CHINESE RES ACAD OF ENVIRONMENTAL SCI
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  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0006] In order to solve the problems of conventional physical adsorption and chemical redox, a combination of physical, chemical, and biological methods can be used to restore groundwater. At present, there is no double layer of groundwater restoration that integrates physical adsorption, oxygen release, and bacterial agent embedding. active medium material

Method used

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  • Bilayer active medium material and preparation method and application method thereof

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preparation example Construction

[0042] The method for preparing the above-mentioned double-layer active medium material provided by the present invention adopts two sets of molds to distribute and shape the inner and outer layer materials, and the main steps are:

[0043] 1) Preparation of ferromanganese oxidizing microbial bacterial liquid: Inoculate Pseudomonas putida, Phytophthora spp., and Bacillus into half-strength LB medium at a weight ratio of 1:0.5-1:0.2-0.5 to activate for 1 day , inoculated into a growth medium containing ferrous manganese to acclimatize until a brown-black precipitate is produced, and then inoculated into an expansion medium to expand culture to obtain a composite bacterial liquid. The medium was sterilized before use.

[0044] 2) Prepare the inner layer material: the mass fraction is 10%-30% oxygen-releasing compound, 10%-20% cement, 5%-15% high permeability material, 10%-30% potassium dihydrogen phosphate, 25%- Mix 45% ammonium sulfate and 4%-8% trace elements evenly;

[0045...

Embodiment 1

[0051] 1) Preparation of ferromanganese oxidizing microbial bacterial liquid: inoculate 0.5g of Pseudomonas putida, 0.4g of Phytophthora spp. Shake at 120rpm in a constant temperature shaking box at 28°C for 1 day, absorb 25mL of bacterial liquid and add it to a conical flask containing 500mL growth medium for acclimatization, place it in a constant temperature shaking box at 28°C and shake at 150rpm until brown-black precipitation occurs, draw 25mL of bacterial liquid to Put 500mL conical flask of expansion medium in a constant temperature shaking box at 28°C at 150rpm and shake for 2 days to obtain a composite bacterial solution.

[0052] The half-strength LB medium is 5g tryptone, 2.5g yeast extract, 5g NaCl dissolved in 1L water, and the growth medium is 1g MnCO 3 , 1gFeCl 2 , 0.15g Fe(NH 4 )(SO 4 ) 2 , 0.075g yeast extract, 0.15g sodium citrate, 0.05gNa 4 P 2 o 7 10H 2 O was dissolved in 1L water, and the expansion medium was 0.5g yeast extract, 0.5g acid hydrolyz...

Embodiment 2

[0059] 1) Preparation of ferromanganese oxidizing microbial bacterial liquid: Inoculate 0.7g of Pseudomonas putida, 0.5g of Phytophthora spp., and 0.4g of Bacillus into an Erlenmeyer flask containing 100mL of half-strength LB medium, and place in Shake at a speed of 120rpm in a constant temperature shaking box at 28°C for 1 day, absorb 25mL of bacterial liquid and add it to an Erlenmeyer flask containing 500mL growth medium for acclimatization, place it in a constant temperature shaking box at 28°C and shake at 150rpm until a brown-black precipitate is produced, draw 25mL Put the bacterial liquid into a 500mL Erlenmeyer flask of expansion medium, place it in a constant temperature shaking box at 28°C and shake it at 150rpm for 2 days, and obtain a composite bacterial liquid.

[0060] The half-strength LB medium is 5g tryptone, 2.5g yeast extract, 5g NaCl dissolved in 1L water, and the growth medium is 1g MnCO 3 , 1gFeCl 2 , 0.15g Fe(NH 4 )(SO 4 ) 2 , 0.075g yeast extract, ...

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Abstract

A bilayer active medium material structurally comprises an inner layer and an outer layer, wherein the thickness ratio of the inner layer and the outer layer is (1-5) to 1, and the permeability coefficient of the inner layer is 10-300 times of that of the outer layer. The inner layer comprises, by weight, 10%-30% of oxygen releasing compound, 10%-20% of cement, 5%-15% of high-permeability materials, 10%-30% of monopotassium phosphate, 25%-45% of ammonium sulfate and 4%-8% of microelements. The outer layer comprises, by weight, iron-manganese oxidized microorganism bacteria liquor, 1%-5% (by mass) sodium alginate, 10%-30% (by mass) high-permeability materials and 10%-20% (by mass) zeolite, wherein the volume ratio of the iron-manganese oxidized microorganism bacteria liquor to the 1%-5% (by mass) sodium alginate is (1-5) to 1, and the iron-manganese oxidized microorganism bacteria liquor is compound bacteria liquor formed by Pseudomonas putida, Leptothrix discophora and bacillus which are subjected to iron-manganese domestication and are in the weight ratio of 1 to (0.5-1) to (0.2-0.5). The invention further discloses a preparation method and application of the bilayer active medium material.

Description

technical field [0001] The invention belongs to the field of groundwater pollution restoration in the field of environmental protection, and in particular relates to a double-layer active medium material for repairing groundwater pollution in landfill sites in areas with high iron and manganese content. [0002] The present invention also relates to a preparation method of the above-mentioned double-layer active medium material. [0003] The invention also relates to the application of the above-mentioned double-layer active medium material in repairing groundwater pollution of landfill sites in areas with high iron and manganese content. Background technique [0004] Groundwater containing iron and manganese is widely distributed in my country, mainly in the Sanjiang Plain, the Lower Liaohe Plain and the middle and lower reaches of the Yangtze River in the Northeast, followed by parts of Heilongjiang, Jilin, Fujian, Jiangxi, Zhejiang and other provinces. In many small and ...

Claims

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Application Information

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IPC IPC(8): C02F3/34
Inventor 席北斗姜永海杨昱廉新颖董子萱马志飞梁冠男
Owner CHINESE RES ACAD OF ENVIRONMENTAL SCI
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