Supercharge Your Innovation With Domain-Expert AI Agents!

Duck tembusu virus attenuated vaccine strain and application thereof

A duck Tembusu virus and attenuated vaccine technology, which is applied in the direction of viruses, antiviral agents, and viral antigen components, can solve the problems of weak cell-mediated immunity, generation of immunity, and no local immunity, so as to reduce the production process , The effect of saving production costs

Pending Publication Date: 2017-01-25
INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
View PDF1 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Vaccines currently used to prevent the disease include inactivated vaccines and attenuated vaccines. As we all know, inactivated vaccines have inevitable disadvantages compared with attenuated live vaccines: they do not produce local immunity; Slower, usually 2 weeks after vaccination to develop good immunity
[0004] At present, there is no commercial attenuated vaccine for ducks, and there is no Tambusu virus vaccine for chickens and geese

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Duck tembusu virus attenuated vaccine strain and application thereof
  • Duck tembusu virus attenuated vaccine strain and application thereof
  • Duck tembusu virus attenuated vaccine strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1: Duck Tembusu virus attenuation

[0017] (1) Subculture on duck embryo fibroblasts:

[0018] Take 11-day-old muscovy duck embryos (without duck Tembusu virus maternal antibody) after disinfection, and perform the following operations under sterile conditions in a biological safety cabinet: Take out the embryo body with high-pressure sterilized forceps and place it in PBS buffer solution After washing twice, cut off the head, neck, limbs and viscera of the duck embryo, cut the remaining embryo body into pieces and then digest it with 0.25% V / V trypsin, and blow the cells with a pipette into a single duck embryo fibroblast, diluted to 10 with 6% V / V calf serum 5 / mL cell density, transfer to T25 (5mL) cell culture flask, at 37°C, 5% CO 2 The cells were cultured in an incubator and used to inoculate duck Tembusu virus WR strain the next day. The virus liquid infected by 1 / 10 of the medium volume, the multiplicity of infection of the virus is 1 ~ 5PFU / cell, ...

Embodiment 2

[0054] Embodiment 2: the preparation of duck Tembusu virus WR strain A83 cell attenuated live vaccine

[0055] ① Basic seed batch establishment

[0056] Take the lyophilized virus seed of the 83rd generation cells, dilute it with the cell culture medium maintenance solution at 1:10, and inoculate BHK-21. When the cytopathy reaches 80%, transfer the cell culture bottle to the -80°C refrigerator and freeze and thaw twice Finally, collect the virus suspension, after the sterility test is up to standard, centrifuge at 12000rpm for 15 minutes, collect the supernatant after centrifugation, and obtain the virus solution containing duck Tembusu virus WR strain A83 as the basic seed, and put it in a -80°C refrigerator Save for later.

[0057] ② Establishment of production seed batches

[0058] Inoculate the basic virus seeds into BHK-21. When the cytopathic effect reaches 80%, transfer the cell culture bottle to -80℃ refrigerator and freeze and thaw twice, collect the virus suspensio...

Embodiment 3

[0086] Embodiment 3: the application of duck Tembusu virus attenuated live vaccine

[0087] Immune laying ducks with attenuated live vaccine of duck Tembusu virus

[0088] 330 laying ducks aged 120-130 days were taken, and 0.1 mL of attenuated live vaccine was injected intramuscularly into each leg. Seven days after immunization, all ducks were collected blood, separated serum and tested for anti-Tambusu virus antibody.

[0089] Results: The average neutralizing antibody titer of 330 laying ducks was 2 5.6 .

[0090] Immune laying hens with attenuated live vaccine of duck Tembusu virus

[0091] 310 laying hens aged 160-170 days were taken, and 0.1 mL of attenuated live vaccine was injected intramuscularly into each leg. Seven days after immunization, blood collection, serum separation and anti-Tambusu virus antibody determination were performed on all geese. The average neutralizing antibody titer of all laying hens after immunization was 2 5.2 .

[0092] Immunization of ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a duck tembusu virus attenuated vaccine strain WR-A83. A microbial collection number of the strain is CGMCC NO.10491. The duck tembusu virus attenuated vaccine strain WR-A83 which is screened is used for preparing attenuated vaccines and inactivated vaccines for preventing chicken, duck and goose tembusu virus. The attenuated vaccine strain WR-A83 has important biological activities like genetic stability and high safety, safe to and free of pathogenicity to chicken, duck and goose and has good immunoprotection effect on tembusu virus in chicken, duck and goose. Vaccines prepared from the attenuated vaccine strain are high in protection rate, safe and effective when in effective dosage.

Description

technical field [0001] The invention relates to a virus attenuated and attenuated vaccine strain, in particular to a duck Tembusu virus attenuated vaccine strain and its application, belonging to the technical field of veterinary biological products. Background technique [0002] Duck Tembusu virus disease is one of the severe infectious diseases caused by duck Tembusu virus (DTV), which causes a sudden drop in egg production of laying ducks and breeding ducks, or even extinction. The laying ducks and breeding ducks with high egg production rate (such as more than 95%) of the disease will drop rapidly from the egg production rate to less than 10% within 1 week after the onset of the disease, and the serious ones will stop production within 10 days. a catastrophic blow. [0003] The most effective way to prevent Tembusu virus is immunization. Vaccines currently used to prevent the disease include inactivated vaccines and attenuated vaccines. As we all know, inactivated vacc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/04A61K39/12A61P31/14
CPCA61K39/12A61K2039/5252A61K2039/5254C12N7/00C12N2770/24021C12N2770/24034
Inventor 傅秋玲黄瑜傅光华陈红梅程龙飞施少华万春和林建生刘荣昌
Owner INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com