Method for producing loach tetraploid

Abstract

The invention discloses a method for producing a loach tetraploid. The male parent fish is a natural tetraploid loach, the female parent fish is a diploid loach, and dry fertilization is adopted; and after the fertilization, the zygote is treated for 50-60min in water at 0 DEG C and then normally hatched in normal-temperature aerated water. In the invention, two loaches different in chromosome number are adopted, namely the diploid loach (2n=50) and the tetraploid loach (4n=100); the release of a second polar body is inhibited through cold shock treatment, and the tetraploid is induced; and the chromosome number, DNA relative content, red blood cell nucleus volume, chromosome number, micro-satellite and the like of the generated offspring are analyzed relatively systematically, so that the authenticity and reliability of tetraploid loach induction are proven, and the method has application values to the protection and utilization of loach genetic resources and further aquaculture of the loaches.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for producing loach tetraploid. Background technique [0002] Fish polyploid breeding is a breeding technology developed on the basis of genome manipulation, and has long been a hot spot in the research of the aquatic industry. The sterility of triploids has been paid more and more attention in the management of fishery resources. It is generally believed that the ability of sterile triploid fish to convert the energy of gonadal development into muscle growth confers a potential growth advantage. In addition, the sterility of triploid fish is also of great significance for controlling the over-breeding of farmed fish and protecting natural germplasm resources, and triploid fish can also be used as an ideal carrier of transgenic fish to solve the ecological security of transgenic fish question. In view of the various advantages of triploid fish, the preparation of triploid...

AI Technical Summary

Problems solved by technology

[0003] In the past, fish tetraploids were artificially induced to achieve chromosome doubling by inhibiting the first cleavage of fertilized eggs through physical, chemical and biological methods after in vitro fertilization. For example, Ge Wenlong doubled the chromosomes by inhibiting the first cleavage. Successful, many tetraploid embryos were observed at the embryonic stage, but larvae could not hatch, or larvae hatched but were mostly deformed Difficult to find live tetraploid fish

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