Method for synchronously extracting cordyceps militaris polysaccharide from composite dual enzymes, and application of method in processing cordyceps militaris beverage

A simultaneous extraction and compound double-enzyme technology, which is applied in the fields of application, food extraction, and the function of food ingredients, can solve the problems of chemical reagent residue and low extraction rate of polysaccharides, so as to improve the extraction rate, enhance immunity, and ensure stability. Effect

Inactive Publication Date: 2017-02-15
SHENYANG INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problems existing in the above-mentioned prior art, the present invention provides a method for synchronously extracting polysaccharides from Cordyceps militaris with compound double enzymes, which solves the problems of low extraction rate of polysaccharides and residual chemical reagents in the prior art

Method used

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  • Method for synchronously extracting cordyceps militaris polysaccharide from composite dual enzymes, and application of method in processing cordyceps militaris beverage
  • Method for synchronously extracting cordyceps militaris polysaccharide from composite dual enzymes, and application of method in processing cordyceps militaris beverage
  • Method for synchronously extracting cordyceps militaris polysaccharide from composite dual enzymes, and application of method in processing cordyceps militaris beverage

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Experimental program
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Embodiment 1

[0024] Example 1 Screening experiment of optimal parameters for enzymatic hydrolysis and extraction of Cordyceps polysaccharide

[0025] 1 Materials and methods

[0026] 1.1 Materials and reagents

[0027] Cordyceps militaris Dalian Jinhe Fungus Industry Co., Ltd.

[0028] Pectinase (food grade) Nanjing Songguan Biotechnology Co., Ltd.

[0029] Cellulase (food grade) Beijing Shengshi Jiaming Technology Development Co., Ltd.

[0030] 1.2 Main instruments and equipment

[0031]

[0032] 1.3 Experimental method

[0033] 1.3.1 Determination of Cordyceps polysaccharide Dried Cordyceps militaris was crushed, passed through a 60-mesh sieve, enzymolyzed to extract the Cordyceps polysaccharide in a 55°C water bath, centrifuged to take the supernatant, repeated the above operation three times, combined the supernatant, and vacuum concentrated to a certain volume. Add 80% final volume of ethanol to precipitate, add water to redissolve the precipitate, use sevage method to remove ...

Embodiment 2

[0076] Example 2 Optimization experiment of sensory quality parameters of Cordyceps beverage

[0077] 1.1 Materials and reagents

[0078]

[0079] 1.2 Main instruments and equipment

[0080]

[0081] 1.3 Experimental method

[0082] 1.3.1 Sensory evaluation The percentage system is used for sensory evaluation. The scoring range of all sensory indicators is 1-25 points. The higher the score, the better the quality. Sensory evaluation index includes mouthfeel, smell, color and luster, viscosity 4 items, and sensory index and scoring standard thereof are as follows: smell (1-9 points: poor; 10-19 points: average; 20-25 points: good), mouthfeel ( 1-9 points: poor; 10-19 points: general; 20-25 points: good), color (1-9 points: colorless; 10-19 points: moderate; 20-25 points: light yellow), viscous Degree (1-9 points: very viscous; 10-19 points: moderate; 20-25 points: good). Select 30 professional beverage appraisers for sensory evaluation, including 15 men and 15 women. ...

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Abstract

The invention relates to a method for synchronously extracting cordyceps militaris polysaccharide from composite dual enzymes. The method comprises the following steps of crushing dried cordyceps militaris, adding water, mixing the crushed cordyceps militaris with the water, adjusting pH, performing enzymolysis and the like so as to obtain a cordyceps militaris stock solution. The cordyceps militaris polysaccharide is extracted by an enzyme method, so that the extraction rate of the cordyceps militaris polysaccharide can be effectively increased, besides, the structure and the composition of the cordyceps militaris are not destroyed, the exertion of the efficacies of the cordyceps militaris is facilitated, and the utilization rate and the absorptivity are increased.

Description

technical field [0001] The invention relates to a method for extracting fungal polysaccharides, in particular to a method for synchronously extracting polysaccharides from Cordyceps militaris with compound double enzymes and its application in processing Cordyceps militaris beverages, belonging to the field of high-tech processing. Background technique [0002] Wang Pu et al focused on the pharmacological activity of Cordyceps polysaccharides through the chemical structure of Cordyceps polysaccharides, especially anti-tumor, hypoglycemic, protective effects on liver and kidney function, and immune regulation, and proposed that Cordyceps polysaccharides can be applied to food and play a role in human health. to a significant effect. He Jianli et al. researched and summarized the detection methods of functional components in Cordyceps, such as cordycepic acid, cordycepin, polysaccharides, sterols, and superoxide dismutase, which can be applied to the quality control and develo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L2/38A23L2/84A23L2/52A23L33/00C08B37/00
CPCA23L2/38A23L2/52A23L2/84C08B37/0003A23V2002/00A23V2200/30A23V2250/208A23V2250/606A23V2250/032A23V2300/14A23V2250/51082A23V2250/5086
Inventor 韩宗元王伟妮李晓静
Owner SHENYANG INST OF TECH
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