Compound nitrogen-fixing microorganism bacterium fertilizer and preparation method thereof

A technology of nitrogen-fixing microorganisms and bacterial fertilizers, which is applied in the preparation of organic fertilizers, fertilizers made from biological waste, nitrogen fertilizers, etc., can solve the problems of poor growth and development, limited ability to adapt to the environment, and difficulty in commercialization, etc., and achieve a wide range of PH adaptability sex, stimulating plant growth, and preventing spore germination

Inactive Publication Date: 2017-02-15
FOSHAN YANHUI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research results show that the application of nitrogen-fixing bacteria fertilizer can promote the growth of plants, accompanied by a certain effect of increasing production, but the effect is unstable. The main reason is that the number of viable bacteria of nitrogen-fixing bacteria decreases during storage and transportation. Affected by the soil environment, the number and activity of viable bacteria will also decrease, and the effect c

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A compound nitrogen-fixing microbial fertilizer, characterized in that the raw material ratio of the compound nitrogen-fixing microbial fertilizer is: 20 parts of Bacillus flexus fermentation broth, 50 parts of Clostridium pasteurianus fermentation broth, and Klebsiella indica 30 parts of fermented liquid.

[0033] A kind of compound nitrogen-fixing microbial bacterial fertilizer according to claim 1, is characterized in that, its preparation method comprises the following steps:

[0034] Step 1, the preparation of Bacillus flexus fermented liquid

[0035] Take out the preservation tube of Bacillus flexus, use nitrogen-free solid medium to draw plates for recovery, and culture at 30°C for 96 hours. Pick a single colony under the plate and inoculate it into a nitrogen-free solid medium, culture it in a 30°C incubator for 72 hours, wash the bacterial lawn in three eggplant bottles with 3000ml sterile water, and inoculate it into a 300L curved In the 500L fermentation ta...

Embodiment 2

[0051] The application effect of the composite nitrogen-fixing microbial bacterial fertilizer prepared in Example 1 on pepper (abbreviated bacterial fertilizer)

[0052] 1 Test time: May-August 2015

[0053] 2. Test location: Zhongqiao Village, Tantou Town, Longhui County, Hunan Province

[0054] 3. experiment method:

[0055] Test group: When transplanting, dip the seedlings with 200 times of bacterial fertilizer dilution for 30 minutes and plant them. After 10 days after planting, use 1000 times of bacterial fertilizer dilution to irrigate the roots once. The total bacteria used is 2 kg / mu, and the total nitrogen fertilizer is 20 kg / mu; the control group dipped and irrigated the roots with clear water, and the total nitrogen fertilizer application was 40 kg / mu;

[0056] 4. test results:

[0057] , The impact on output and economic benefits The results found that the average yield of the compound nitrogen-fixing microbial fertilizer was 726 kg / mu, and the average yiel...

Embodiment 3

[0062] Application effect of compound nitrogen-fixing microbial fertilizer on cucumber

[0063] 1. Test location: a grower in Shouguang City, Shandong Province

[0064] 2. Test method: Test group: When transplanting, dip the seedlings with a 200-fold dilution of bacterial fertilizer for 20 minutes to plant, and 10 days after planting, irrigate the roots once with a 1000-fold dilution of bacterial fertilizer, and the total amount of bacterial fertilizer applied is 2 kg. Nitrogen fertilizer is 15 kilograms; Control group: dip root with clear water and irrigate root, and total nitrogen fertilizer is 30 kilograms / mu;

[0065]3. Test results: After transplanting, it was found that the leaves of the plants with roots were fresher, and the growth potential was good, and there was no wilting. The plants using the compound nitrogen-fixing microbial fertilizer showed flowers one week earlier than the control group. After most of the flowers have bloomed, it is found that the flowers...

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Abstract

The invention discloses a compound nitrogen-fixing microorganism bacterium fertilizer and a preparation method thereof, particularly a compound nitrogen-fixing microorganism bacterium fertilizer which is wide in PH application range, strong in nitrogen fixing capacity under aerobic and anaerobic conditions, and easy to store. The compound nitrogen-fixing microorganism bacterium fertilizer is prepared from the following raw materials: 10 to 30 parts of bacillus flexus fermenting liquid, 30 to 80 parts of clostridium pasteurianum fermenting liquid and 20 to 60 parts of India Beijerinckia fermenting liquid. According to the compound nitrogen-fixing microorganism bacterium fertilizer and the preparation method thereof, the aerobic and anaerobic nitrogen fixing bacteria series are fused into a whole creatively; one type is the aerobic nitrogen fixing bacteria, namely, the India Beijerinckia (the India Beijerinckia can fix nitrogen under low oxygen, but is mainly the aerobic bacterium) and the bacillus flexus; the other type is the anaerobic nitrogen fixing bacteria, namely, the clostridium barati; both the aerobic nitrogen fixing bacteria and the anaerobic nitrogen fixing bacteria have strong nitrogen fixing capacity, can fix the nitrogen in relatively deep soil (10 to 30 cm), and can convert nitrogen in air into the nitrogen which can be absorbed by a plant and provide the nitrogen to the plant continuously.

Description

technical field [0001] The invention belongs to the technical field of biological fertilizers, and relates to a compound nitrogen-fixing microbial bacterial fertilizer and a preparation method thereof. Background technique [0002] Nitrogen-fixing bacteria fertilizers refer to microbial strains that live in soil or crop rhizosphere and fix nitrogen in the air. They are living products made by submerged liquid fermentation. They can fix nitrogen in the air as nutrients and secrete auxin Substances help crops grow. In nature, about 100 million tons of nitrogen are fixed by microorganisms every year, which plays a great role in increasing soil fertility. The research results show that the application of nitrogen-fixing bacteria fertilizer can promote the growth of plants, accompanied by a certain effect of increasing production, but the effect is unstable. The main reason is that the number of viable bacteria of nitrogen-fixing bacteria decreases during storage and transportati...

Claims

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Application Information

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IPC IPC(8): C05G3/00C05F17/00
CPCC05B7/00C05F17/00C05G3/00C05C3/00C05C11/00C05D3/02C05D5/00C05D9/00C05D9/02C05F1/007C05F5/006C05F11/00C05F11/08Y02W30/40
Inventor 蒋常德胡艳晖
Owner FOSHAN YANHUI BIOTECH CO LTD
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