A method for biodegrading lutein to generate 8-methyl-α-ionone

A technology of ionone and biodegradation, applied in the field of microbial fermentation, to achieve the effects of low production cost, good social and economic benefits, and mild reaction conditions

Active Publication Date: 2019-10-29
ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, biodegradation of lutein will attract more and more attention, but there are still few studies on the use of enzymes and microorganisms to degrade lutein to produce aroma substances.
At present, there is no report on the use of Enterobacter hallii to degrade lutein to produce 8-methyl-α-ionone

Method used

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  • A method for biodegrading lutein to generate 8-methyl-α-ionone
  • A method for biodegrading lutein to generate 8-methyl-α-ionone
  • A method for biodegrading lutein to generate 8-methyl-α-ionone

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Embodiment 1

[0032] The screening of embodiment 1 lutein concentration

[0033] First configure the fermentation medium for lutein degradation: glucose 3g, asparagine 4.5g, yeast powder 3g, K 2 HPO 4 1.5g, MgSO 4 0.5g, FeSO 4 0.01g, 1L of distilled water, adjust the pH to 7.0, put each 100mL of fermentation medium into a 250mL Erlenmeyer flask, seal it, sterilize at 121°C for 20min, cool to room temperature for use.

[0034] Preparation of lutein solution: Disperse 200mg of lutein in 2g Tween 80 to obtain a lutein dispersion, then dissolve the lutein dispersion in 400mL of dichloromethane, and dissolve the dichloromethane Evaporate under reduced pressure, and dissolve the residue after decompressed evaporation in 10 mL of ethanol; pass the lutein solution through a 0.45 μm organic filter to remove internal bacteria, and set aside.

[0035] Biodegradation process: Add the lutein solution to multiple fermentation media respectively until the final concentrations of lutein are 300, 400,...

Embodiment 2

[0049] The mensuration of embodiment 2 lutein degradation products

[0050] Degradation products of lutein fermented and degraded by Enterobacter hallii of the present invention are determined, and the specific method is as follows:

[0051] The fermentation broth was centrifuged at high speed (4°C, 6500r / min, 30min) under light-shielding conditions, and the separated supernatant was taken out, and an equal volume of dichloromethane was added to extract repeatedly 3 times to obtain the lutein fermentation extract. Add anhydrous Na 2 SO 4 Dry overnight, concentrate in vacuo to 1 mL, and perform GC-MS qualitative analysis.

[0052] Gas phase conditions: Chromatographic column: HP-5 (30m×0.25mm; 0.25μm); heating program: initial temperature of 45°C, keep for 5min, rise to 110°C at 4°C / min, hold for 5min, rise to 1°C / min 150°C, hold for 5min, rise to 200°C at 3°C / min, rise to 280°C at 5°C / min, hold for 5min; inlet temperature: 270°C; transfer line temperature: 270°C; split rati...

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Abstract

The invention discloses a method for production of 8-methyl-alpha-ionone by biodegradation of lutein. The method utilizes Enterobacter hormaechei for degradation of lutein to generate 8-methyl-alpha-ionone. The method provided by the invention employs Enterobacter hormaechei for the first time to degrade lutein so as to generate the aromatic substance 8-methyl-alpha-ionone, opens up a new way for production of aromatic substances through biodegradation of lutein, solves the technical problems of difficult separation, many reagents and environmental burden in the traditional method of physical-chemical degradation of lutein to generate aromatic substances, and has the characteristics of mild reaction conditions, easy separation, green and environmental protection, etc. By comparing the degradation effects of different substrate concentrations, the invention finds that an optimal lutein degradation effect can be achieved when the concentration of in a fermentation medium is 400-700mg / L.

Description

technical field [0001] The invention relates to a method for biodegrading lutein, in particular to a method for generating 8-methyl-α-ionone by biodegrading lutein with Enterobacter hallii, belonging to the technical field of microbial fermentation. Background technique [0002] Lutein, also known as plant lutein, is called Lutein in English, and its molecular formula is C 40 h 56 o 2 , with a relative molecular weight of 568.85, is a kind of oxygen-containing carotenoid. Lutein is widely distributed in nature, and many vegetables, fruits, such as beans, yellow peaches, and marigolds are rich in lutein. Lutein is the precursor of many important aroma components. During the ripening, conditioning, alcoholization and burning of tobacco leaves, lutein can be degraded and transformed to form a variety of aroma components, such as β-damascenone, ionone and Isophorone, etc. These ingredients have a strong floral and fruity aroma with a low aroma threshold, and are of great va...

Claims

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Application Information

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IPC IPC(8): C12P7/26C12R1/01
CPCC12P7/26
Inventor 杨雪鹏汪芳芳叶建斌马科毛多斌
Owner ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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