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A method for detecting reduced glutathione based on an electrochemical probe

A technology of oxidized glutathione and glutathione, which is applied in the direction of material electrochemical variables, etc., can solve the problems of improving detection sensitivity and achieve the effects of easy purchase, high sensitivity, and easy control of reaction conditions

Active Publication Date: 2020-04-03
GUILIN UNIV OF ELECTRONIC TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the detection sensitivity needs to be improved

Method used

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  • A method for detecting reduced glutathione based on an electrochemical probe
  • A method for detecting reduced glutathione based on an electrochemical probe

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Embodiment Construction

[0025] The present invention will be further described below in conjunction with the accompanying drawings and tables, but the present invention is not limited thereto.

[0026] The experimental methods described in the following examples, unless otherwise specified, are the reagents and materials described in conventional methods, and all of them can be obtained from commercial sources unless otherwise specified.

[0027] The present invention is completed on a CHI660D electrochemical workstation, the working electrode is a gold electrode, the counter electrode is a platinum electrode, and the reference electrode is a silver / silver chloride electrode. The electrochemical test is differential pulse voltammetry (DPV), and the experimental condition is that the supporting electrolyte is 1.0mol / L Na 2 SO 4 , with H 2 SO 4 and NaOH to adjust the pH value of the solution to 6.0, and the test temperature was 25°C. A method for highly sensitive detection of reduced glutathione ba...

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Abstract

The invention provides a method for detecting reduced glutathione based on electrochemical probe with high sensitivity. Rare earth cerium (IV) is used as an electrochemical probe, an oxidation reduction reaction is carried out based on Ce(IV) and GSH, when Ce(IV) is converted into Ce(III), the electrochemical signal is changed, and a method for detecting reduced glutathione by using electrochemical sensor technology is established. The method is completed in a CHI660D electrochemical work station, a working electrode is a gold electrode, a counter electrode is a platinum electrode, a reference electrode is a silver / silver chloride electrode, an electrochemical test method is a differential impulse method, and the experimental condition is as follows: supporting electrolyte is 1.0mol / L Na2SO4, the pH value of the solution is 6, and the testing temperature is 25 DEG C; a DPV method is used for separately detecting changes of a current value. The current value and the concentration of added GSH have a linear relation, the detection concentration of GSH is obtained by calculation, and the detection limit is 0.05nmol.L<-1>.

Description

technical field [0001] The invention belongs to the technical field of electrochemical sensors, in particular to a method for detecting reduced glutathione. Background technique [0002] Low-molecular-weight sulfhydryl compounds (RSH), such as cysteine ​​(Cystine, Cys) and glutathione (Glutathione, GSH), have reducing properties and play a very important role in the antioxidant defense system and the control of various functions of proteins in vivo. important role. Cysteine ​​is an important sulfur-containing amino acid, which has a lot of medicinal value, can alleviate drug poisoning, and also has a preventive effect on radiation damage; reduced glutathione itself is easily oxidized by certain substances, so it can Protect the sulfhydryl groups in molecules such as many proteins and enzymes from being oxidized by harmful substances such as free radicals, so that molecules such as proteins and enzymes can perform their physiological functions. Quantitative detection of sul...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/48
CPCG01N27/48
Inventor 韩国成冯小珍侯嘉婷陈真诚周治德
Owner GUILIN UNIV OF ELECTRONIC TECH