Composition and method for identifying pasteurella multocida and/or haemophilus parasuis

Abstract

The invention discloses a composition for identifying pasteurella multocida and haemophilus parasuis at the same time, a kit and an identifying method. The composition at least comprises the following two primer pairs, one primer pair is selected from at least one primer pair of a primer pair set composed of a PCR primer pairs composed of two single stranded DNAs shown in the sequence 1 and the sequence 2 in a sequence table and a PCR primer pair composed of two single stranded DNAs shown in the sequence 5 and the sequence 6 in the sequence table, and the other primer pair is selected from at least one primer pair of a primer pair set of a PCR primer pair composed of two single stranded DNAs shown in the sequence 3 and the sequence 4 in the sequence table, a PCR primer pair composed of two single stranded DNAs shown in the sequence 7 and the sequence 8 in the sequence table and a PCR primer pair composed of two single stranded DNAs shown in the sequence 9 and the sequence 10 in the sequence table. Pathogene can be accurately identified, the mixed infection condition can be analyzed, the identifying speed is high, cost is low, and the application range is wide.

Description

technical field [0001] The invention relates to a composition and a multiplex PCR method in the field of biotechnology, in particular to a composition and a multiplex PCR method capable of simultaneously identifying Pasteurella multocida and / or Haemophilus parasuis. Background technique [0002] Studies have shown that porcine respiratory disease is a disease caused by mixed infection of one or more bacteria and viruses. Usually, viruses or mycoplasma first invade the respiratory tract, destroying the defense barrier, and various airborne pathogens such as Pasteurella multocida and Haemophilus parasuis enter the respiratory tract and lungs, causing secondary or mixed infections. Pasteurella multocida is a Gram-negative bacterium that mainly causes acute hemorrhagic sepsis, porcine pneumonia and atrophic rhinitis in pigs, often in mixed infection or secondary infection with other viruses and bacteria. [0003] Haemophilus parasuis is a Gram-negative bacterium that can cause ...

AI Technical Summary

Problems solved by technology

The current isolation and identification of Haemophilus parasuis is more than 20% less than the actual infection rate of the farm. It is usually mixed with pathogens such as Pasteurella multocida and porcine reproductive and respiratory virus. Due to its harsh culture conditions, it limits research on it

[0004] Pasteurella multocida and Haemophilus parasuis are common and important pathogens in the respiratory tract of pigs, and the clinical symptoms caused by them are difficult to distinguish

For the identification of Pasteurella multocida and Haemophilus parasuis, traditional methods such as morphological observation, physiological and biochemical identification, and immunological identification are mostly used. These methods are time-consuming, labor-intensive and have poor accuracy. Methods and clinical experience are difficult to judge the pathogen, which will delay the best time for the prevention and control of animal diseases; therefore, it is necessary to establish an accurate and rapid method for identifying the above two common pathogens in the respiratory tract of pigs

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