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Method for removing small-molecule substance from serum

A small molecule, serum technology, applied in the field of in vitro diagnostic medical testing, can solve the problems of lack of advantages, poor reproducibility of the kit, and lack of market competitiveness.

Active Publication Date: 2017-02-22
SHANGHAI KEHUA BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the plate chemiluminescence method solves the shortcomings of the low quantitative sensitivity of the enzyme-linked immunosorbent assay, it is not suitable for a fully automatic immunoluminescence instrument, and has no advantage over imported kits
Most of the kits produced by domestic reagent manufacturers are still enzyme-linked immunoassay kits (ELISA), which lack market competitiveness. The four major foreign in vitro diagnostic reagent companies, such as Roche, Abbott, Beckman, and Siemens, are monopolized. This is one of the reasons for the high cost of detection and diagnosis, and the preparation methods and related materials of the kits are kept as confidential information and have not been made public, which is not conducive to Promotion of total triiodothyronine and free triiodothyronine detection in community primary hospitals
Domestic total triiodothyronine and free triiodothyronine kits have poor reproducibility, difficult to control batch-to-batch differences, low sensitivity, high cross-reaction rate, and poor clinical application prospects

Method used

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  • Method for removing small-molecule substance from serum
  • Method for removing small-molecule substance from serum
  • Method for removing small-molecule substance from serum

Examples

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preparation example Construction

[0053] Correspondingly, the present invention also provides a method for preparing the above-mentioned dethyroid hormone serum, the method comprising the following steps:

[0054] a) using the above-mentioned dextran-treated activated carbon to process human serum;

[0055] b) After mixing the dextran-treated activated carbon and human serum system obtained in step a) to remove thyroid hormones in the human serum, centrifuge at low speed to remove the dextran-treated activated carbon.

[0056] In the present invention, the treatment is to mix the dextran-treated activated carbon with normal human serum.

[0057] The treatment is to mix dextran-treated activated carbon with normal human serum at a concentration of 20-40mg / ml at 2-8°C for 2-7 days; preferably 40mg / ml for 3 days or 20mg / ml ml, after 2 days, add 10mg / ml and mix for 1 day. In view of the teaching of the present invention and the prior art, those of ordinary skill in the art can reasonably determine the appropriat...

Embodiment 1

[0080] Example 1. Preparation of activated carbon treated with dextran

[0081] Select 1 g of dextran with a molecular weight of 10,000 Daltons, add 20 mL of purified water to fully dissolve it, add 10 mg of 20A activated carbon with a particle size of 0.6 mm, and a pore size of 100 mm, and mix slowly overnight. After the activated carbon is completely sealed, wash it repeatedly with water , drained to obtain dextran-treated activated carbon.

Embodiment 2

[0082] Example 2. Treatment of human serum with activated carbon treated with dextran to obtain dethyroid hormone serum

[0083] Mix 10 g of dextran-treated activated carbon with 250 mL of serum, mix at 2-8°C at 50 rpm for 3 days, and centrifuge the hormone-depleted serum at 2,500 rpm to obtain dethyroid hormone serum after removing the activated carbon.

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Abstract

The invention discloses a method for removing a small-molecule substance from serum, in particular thyroid hormone removing serum, a preparation method thereof, and a human total triiodothyronine and free thyronine detection kit comprising the thyroid hormone removing serum. The thyroid hormone removing serum in the invention is obtained by activated carbon adsorption of glucan-treated serum. The human total triiodothyronine and free thyronine detection kit in the invention has the advantages of high detection sensitivity, high specificity, high result stability and the like, so as to be applicable to clinical auxiliary diagnosis screening for thyroid diseases on a large scale.

Description

technical field [0001] The invention relates to the field of in vitro diagnostic medical testing. Specifically, the present invention relates to a method for removing small molecule substances in serum, such as thyroid hormone in serum, and the use of the prepared serum free of small molecule substances. Background technique [0002] In the field of in vitro diagnostic medical testing, the so-called calibrator refers to the standard substance used by the client as a standard substance suitable for a specific reagent. For example, in order to detect a certain substance in serum, in order to avoid the error of different detection substances, it is generally necessary to remove the serum as the calibrator diluent. In the development process of the kit, the company establishes the main curve of each batch of kits through the calibrators and assigns values ​​to the calibrators in the product. The client uses the calibrators to correct the main curve to generate a calibration cur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/38
CPCG01N1/38G01N2001/386
Inventor 孙臻懿潘洁彭珊李基赵亚茹
Owner SHANGHAI KEHUA BIO ENG
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