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Methamphetamine detection method and biosensor

A methamphetamine and biosensor technology, which is applied in instruments, measuring devices, scientific instruments, etc., can solve the problems of expensive equipment and instruments, complex sample preparation and pretreatment technology, and the inability to realize on-site rapid detection, and achieve high sensitivity and convenience Detection effect

Inactive Publication Date: 2017-02-22
北京未名清源环保科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these techniques have high sensitivity and good selectivity, they require complex sample preparation and pretreatment techniques, expensive equipment and instruments, and professional technicians
In practical applications, sometimes in order to determine whether a suspect is taking drugs, it is necessary to quickly obtain test results or even on-site testing to prevent drug abuse, but none of the methods mentioned above can achieve on-site rapid testing.

Method used

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  • Methamphetamine detection method and biosensor
  • Methamphetamine detection method and biosensor
  • Methamphetamine detection method and biosensor

Examples

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Embodiment 1

[0032] Embodiment one detects the detection method of methamphetamine

[0033] like figure 1 The detection mechanism of methamphetamine is shown: the active site of DNase with peroxidase activity is mainly composed of two separate chains: 5'-AGG GAC GGG-3' and 5'-GTGGAGGGT-3' ( figure 1 The light-colored parts of the two ends of the longer chain, that is, the parts above and below the intersection of the two chains), in addition to these two chains, the DNase molecular beacon also includes an oligonucleotide ( figure 1 The black part in the middle of the longer chain, i.e. the part between the intersection of the two chains), is located between the two catalytically active chains. This oligonucleotide can partially complement the methamphetamine aptamer to form double-stranded DNA through the principle of complementary base pairing. During the analysis, the DNase molecular beacon itself has peroxidase activity due to the DNase that can form a G-quadruplex-heme structure in t...

Embodiment 2

[0046] Example 2 Biosensor for the detection of methamphetamine

[0047] According to the above-mentioned detection mechanism of methamphetamine, the present application also designs a biosensor of methamphetamine based on colorimetric analysis, which is simple and fast and does not require complicated or expensive equipment.

[0048] The biosensor for methamphetamine detection includes:

[0049] Double-stranded DNA formed by mixing DNase molecular beacon and methamphetamine aptamer in HEPES buffer solution;

[0050] hemin;

[0051] A colorimetric substance formed by mixing ABTS and hydrogen peroxide.

[0052] Wherein the double-stranded DNA is made by the following methods: the DNase molecular beacon and the methamphetamine aptamer are each dissociated at a first set temperature and cooled to room temperature after a first predetermined time; the DNase molecular beacon and The methamphetamine aptamer was mixed in HEPES buffer to form a mixture and incubated at a second set...

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Abstract

The invention relates to a methamphetamine detection method and a biosensor. The method comprises steps as follows: a DNA polymerase molecular beacon and a methamphetamine aptamer are dissociated at respective set temperatures for a preset time and cooled to the room temperature; the DNA polymerase molecular beacon and the methamphetamine aptamer are mixed in an HEPES buffer solution, a mixed solution is formed and incubated at the set temperature for a preset time, so that double-stranded DNA is formed; a to-be-detected object is added to the mixed solution and incubated for a preset time; a colorimetric substance is added to the mixed solution, and whether methamphetamine exists in the to-be-detected object is determined according to color development. With the adoption of the method, methamphetamine is detected simply, rapidly and conveniently, and the method has the advantages that the sensitivity is high and interference from other substances is avoided.

Description

[0001] 【Technical field】 [0002] The invention belongs to the technical field of environmental detection, and in particular relates to a detection technology for methamphetamine in sewage and environmental water bodies. [0003] 【Background technique】 [0004] According to the 2015 World Drug Report and other related literature, illicit drugs are being produced and abused on a worryingly large scale around the world. Among these numerous illegal drugs, methamphetamine (also known as methamphetamine, crystal meth) is the most widely abused drug worldwide after marijuana. Due to its increasing abuse, it has brought a huge threat to human health, because the neurotoxic methamphetamine has irreversible damage to the human central nervous system. If methamphetamine can be tested simply, quickly and inexpensively, it may be able to effectively prevent its abuse to some extent, but there is currently no such an effective detection method. [0005] In the prior art, there are some m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/31
CPCG01N21/78G01N21/3151
Inventor 李喜青毛康
Owner 北京未名清源环保科技有限公司