Chlorinated isocoumarin derivative and preparation method and application thereof

A technology of isocoumarin and derivatives, applied in botany equipment and methods, chemicals for biological control, applications, etc., can solve problems such as chlorinated isocoumarin compounds that have not been seen yet, and achieve inhibition of banana anthracnose bacteria and rice sheath blight, broad application prospects, strong effect of banana anthracnose and rice sheath blight

Active Publication Date: 2017-03-01
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, until now, no chlorinated isocoumarin compounds with important activity against phytopathogenic fungi obtained from plant-derived endophytic fungi have been used as fungicides

Method used

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  • Chlorinated isocoumarin derivative and preparation method and application thereof
  • Chlorinated isocoumarin derivative and preparation method and application thereof
  • Chlorinated isocoumarin derivative and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Chloroisocoumarin derivatives were prepared by the following steps:

[0030] (1) In the culture medium, the endophytic fungus Trichoderma sp.09 is first cultured. The medium used is to contain glucose 1.0% (weight percentage, the same below), yeast extract 0.02%, peptone 0.1%, agar 1.0%, sodium chloride 0.2%, and the rest is water. When used, it is made into a test tube slope, and the above bacteria are cultivated at 28°C for 7 days;

[0031] (2) Trichoderma sp.09 was fermented and cultured, and the culture medium used contained 50.0% of rice, 1.5% of calcium chloride, 1.0% of sodium chloride and the remainder of water, and was cultured statically at room temperature at 25°C for 40 days;

[0032] (3) Extract the strain fermented in step (2) with ethanol-water mixture (95:5) for 3 times, concentrate under reduced pressure, and extract several times with ethyl acetate (ethyl acetate can be replaced by n-butanol) , concentrate the extract, carry out chromatographic separa...

Embodiment 2

[0034] Chloroisocoumarin derivatives were prepared by the following steps:

[0035] (1) In the culture medium, the endophytic fungus Trichoderma sp.09 is first cultured. The medium used is to contain glucose 2.5% (weight percentage, the same below), yeast extract 0.2%, peptone 0.3%, agar 1.0%, sodium chloride 0.5%, and the rest is water. When used, it is made into a test tube slope, and the above bacteria are cultivated at 28°C for 3 days;

[0036] (2) Carry out fermentation culture to Trichoderma sp.09 again, the culture medium used is to contain rice 30.0%, calcium chloride 1.5%, sodium chloride 2.0% and water balance, at 30 ℃ for static culture for 60 days;

[0037] (3) Extract the strain fermented in step (2) with methanol-water mixture (90:10) twice, concentrate under reduced pressure, and extract with n-butanol several times (n-butanol can be replaced by ethyl acetate) , concentrate the extract, carry out chromatographic separation in a 200-300 mesh silica gel column, a...

Embodiment 3

[0039] Chloroisocoumarin derivatives were prepared by the following steps:

[0040](1) In the culture medium, the endophytic fungus Trichoderma sp.09 is first cultured. The medium used is to contain glucose 3.5% (weight percentage, the same below), yeast extract 0.5%, peptone 1.0%, agar 1.5%, sodium chloride 1.5%, and the rest is water, which is made into a test tube slope when used, and the above bacteria are cultivated at 28°C for 5 days;

[0041] (2) Trichoderma sp.09 was fermented and cultured, and the culture medium used contained 40.0% rice, 0.5% calcium chloride, 2.5% sodium chloride and the remainder of water, and was cultured statically at 30°C for 50 days;

[0042] (3) Extract the strain fermented in step (2) with ethanol-water mixture (80:20) 4 times, concentrate under reduced pressure, and extract with n-butanol several times (n-butanol can be replaced by ethyl acetate) , concentrate the extract, carry out chromatographic separation in a 200-300 mesh silica gel co...

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Abstract

The invention discloses a chlorinated isocoumarin derivative and a preparation method and an application thereof, which belong to the field of biochemical industry. A molecular formula of the derivative is C16H14Cl2O7, and a structural formula is shown in a specification. The preparation method comprises the following steps: myoporum bontioides endophytic fungi Trichoderma sp. 09 is subjected to bacterial strain cultivation in a bacterial strain medium, the bacterial strain is subjected to fermentation cultivation in a fermentation medium, the obtained mycelia is subjected to extraction by ethanol-water or methanol-water, and then is performed with vacuum concentration, the mycelia is extracted by an organic solvent extraction; an extract is concentrated and is subjected to column chromatography on silica gel and Sephdex LH-20 gel column chromatography, an eluate is concentrated and recrystallized to obtain the white crystals which are the chlorinated isocoumarin derivative. The chlorinated isocoumarin derivative has strong effect for inhibiting gloeosporium musarum and rice sheath blight fungus and can be used as a microbe agricultural bactericide, the raw materials enable large scale production, and the application prospect is wide.

Description

technical field [0001] The invention belongs to the field of biochemical industry, and relates to a chlorinated isocoumarin derivative and its preparation method and application, in particular to a method against banana anthracnose (Colletotrichum musae) and rice sheath blight (Rhizoctonia solani) ) Chlorinated isocoumarin derivatives with strong inhibitory activity, preparation method and application thereof. Background technique [0002] Plant diseases have always been one of the main restrictive factors for high-quality and high-yield crops. According to statistics, the average annual loss of agricultural production due to diseases in the world accounts for about 10% to 15% of the total output, and the annual direct economic loss is as high as hundreds of billions of dollars. Diseases caused by phytopathogenic fungi account for about 70-80% of plant diseases. They invade some parts of plants and cause bad symptoms such as wilting, necrosis, and rot. For example, rice she...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D407/06C12P17/16A01N43/16A01P3/00
CPCA01N43/16C07D407/06C12P17/162
Inventor 李春远丁唯嘉
Owner SOUTH CHINA AGRI UNIV
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