A method for preparing metal nanoclusters, nanoclusters prepared by the method, and contrast agents containing the nanoclusters
A technology of metal nanoclusters and metal salts, which is applied in the field of metal nanoclusters and its preparation, can solve problems such as poor stability, loss, and misjudgment of PET imaging results, and achieve the goals of improving deposition, improving stability, and ensuring accuracy Effect
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[0037]The present invention provides a method for preparing metal nanoclusters, comprising: providing a mixed solution, the mixed solution including a metal salt, a template agent or a template agent containing a disease-targeting ligand; making the mixed solution system undergo redox reaction, to reduce the metal ion contained in the metal salt to a metal atom, and the metal atom acts on the template to form the metal nanocluster; wherein, the template is selected from protein, peptide, polymer or DNA.
[0038] Specifically, the molar ratio of template agent to metal salt is preferably (1-5):(0.1-0.5), more preferably 2:0.3.
[0039] Preferably, by adding a strong base or a reducing agent to the mixed liquid, the mixed liquid system can undergo a redox reaction, the strong base can be a water-soluble strong base, such as NaOH or KOH, and the reducing agent Can be NaBH 4 .
[0040] The metal salt may include radioactive metal ions and non-radioactive metal ions, the radioac...
Embodiment 1
[0056] Embodiment 1, the preparation of Cu nanocluster
[0057] 1mL, 20mM CuCl 2 Mix the solution with 5mL, 40mg / mL BSA or 5mL, 42mg / mL BSA-LHRH protein solution, stir at room temperature for 2-3min, add 0.4mL, 0.5M NaOH solution to adjust the pH value to 12, the color of the solution changes from blue to purple , the formed solution continued to be stirred at 55° C. for 5 h, and the synthesized Cu nanoclusters were ultrafiltered with an ultrafiltration tube with a cutoff of 3000 to remove free metal ions. The synthesized Cu nanoclusters are represented as: Cu NC @BSA, Cu NC @BSA-LHRH. Cu NC @BSA and Cu NC @BSA-LHRH fluoresces purple under visible light and blue under UV light.
[0058] like Figure 1a As shown, HRTEM shows that the synthesized nanoclusters have an ultra-small particle size and good dispersion, with a particle size of about 3nm. Figure 1b The indicated particle size, as determined by DLS, is approximately 3.5 nm. Figure 1c The shown UV and fluorescen...
Embodiment 2
[0063] Embodiment 2, radioactive [ 64 Preparation of Cu]Cu Nanoclusters
[0064] Will 64 CuCl 2 (555 millibeck (MBq), 80 μL) with 920 μL, 20 mM CuCl 2 The solutions are mixed to produce a metal salt solution. Mix 5mL, 40mg / mL BSA or 5mL, 42mg / mL BSA-LHRH protein solution with the above metal salt solution, stir at room temperature for 2-3min, add 0.4mL, 0.5M NaOH solution to adjust the pH value of the above mixture to 12, The color of the mixed solution changed from blue to purple, and the formed solution was stirred at 55°C for 5h to obtain [ 64 Cu]Cu nanoclusters. Finally, the synthesized [ 64 Cu]Cu nanoclusters were ultrafiltered with an ultrafiltration tube with a cutoff of 3000 to remove free metal ions. Synthetic[ 64 Cu]Cu nanoclusters are expressed as: [ 64 Cu]Cu NC @BSA, [ 64 Cu]Cu NC @BSA-LHRH. After compositing ends, [ 64 Cu]Cu NC @BSA and [ 64 Cu]Cu NC The radiolabeling efficiencies of @BSA-LHRH all reached above 95%.
[0065] The synthesized [ 64...
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