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Method for isolating and preparing rosmarinic acid from Dracocephalum heterophyllum and application thereof

A technology of isophyllum and rosmarinic acid, applied in the field of preparation of natural active ingredients, can solve the problems of cumbersome preparation process, low extraction rate and purity of rosmarinic acid, large amount of organic solvent, etc., and achieve simple process and stable quality Controllable, easy-to-operate effects

Inactive Publication Date: 2017-03-08
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Chinese patents CN 1995007 B, CN 101863767 A, CN 104710311 A and CN 101020637 B disclose the process and preparation method of rosmarinic acid from plants such as rosemary, Prunella vulgaris, Mint, Salvia miltiorrhiza, but the preparation process is cumbersome, The amount of organic solvent is large, and the extraction rate and purity of rosmarinic acid are low

Method used

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  • Method for isolating and preparing rosmarinic acid from Dracocephalum heterophyllum and application thereof
  • Method for isolating and preparing rosmarinic acid from Dracocephalum heterophyllum and application thereof
  • Method for isolating and preparing rosmarinic acid from Dracocephalum heterophyllum and application thereof

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Embodiment 1

[0032] Embodiment 1 The method for isolating and preparing rosmarinic acid from Green Orchid isophyllum comprises the following steps:

[0033] ⑴ Extraction:

[0034] Soak 1 kg of Qinglan herb powder in an ethanol solution 20 times its mass and with a volume concentration of 45% for 10 hours, reflux extraction at a temperature of 75°C for 3 times, each time for 1 hour, and combine to obtain an extract, which is placed in a vacuum 0.05 MPa, the temperature is 60 ℃, and concentrated under reduced pressure to obtain 103.52 g of the ethanol extract of Qinglan isophyllum.

[0035] ⑵Microporous resin impurity removal:

[0036] After dissolving the ethanol extract of Qinglan isophyllum with 20 times of its mass and 10% volume fraction, filter to obtain filtrate A; MCI microporous resin column on filtrate A, with pure water of 3 times of column volume, 3 times of column volume And volume concentration is the ethanol solution of 50%, 3 times of column volumes and the ethanol solution...

Embodiment 2

[0041] Embodiment 2 The method for isolating and preparing rosmarinic acid from Green Orchid isophyllum comprises the following steps:

[0042] ⑴ Extraction:

[0043] Soak 10 kg of Qinglan herb powder in an ethanol solution 5 times its mass and with a volume concentration of 95% for 15 hours, reflux extraction once at 50°C for 3 hours each time, and combine to obtain an extract, which is placed in a vacuum 0.08 MPa, temperature 40 ℃ and concentrated under reduced pressure to obtain 992.37 g of the ethanol extract of Qinglan isophyllum.

[0044] ⑵Microporous resin impurity removal:

[0045] After dissolving the ethanol extract of Qinglan isophyllum with 10 times of its mass and 20% volume fraction, filter to obtain filtrate A; MCI microporous resin column on filtrate A, with 3 times of column volume of pure water, 3 times of column volume And volume concentration is 50% ethanol solution, 3 times of column volumes and volume concentration is 90% ethanol solution elution, colle...

Embodiment 3

[0050] Embodiment 3 The method for isolating and preparing rosmarinic acid from Green Orchid isophyllum comprises the following steps:

[0051] ⑴ Extraction:

[0052] Soak 5 kg of Qinglan herb powder in an ethanol solution 10 times its mass and with a volume concentration of 70% for 12 hours, reflux and extract twice at 65°C for 2 hours each time, and combine to obtain an extract, which is placed in a vacuum 0.07MPa, temperature 50 ℃ and concentrated under reduced pressure to obtain 497.68g of ethanol extract of Qinglan isophyllum.

[0053] ⑵Microporous resin impurity removal:

[0054] After dissolving the ethanol extract of Qinglan isophyllum with 15 times its mass and 15% volume fraction, filter to obtain filtrate A; MCI microporous resin column on filtrate A, with 3 times of column volume pure water, 3 times of column volume And volume concentration is 50% ethanol solution, 3 times of column volumes and volume concentration is 90% ethanol solution elution, collects 50% et...

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Abstract

The invention relates to a method for isolating and preparing rosmarinic acid from Dracocephalum heterophyllum, comprising the steps of (1) extracting, to be specific, soaking powder of Dracocephalum heterophyllum herb, and performing reflux extraction and vacuum concentration to obtain Dracocephalum heterophyllum extract; (2) cleaning by microporous resin, to be specific, dissolving the Dracocephalum heterophyllum extract, filtering, feeding to MCI microporous resin columns, eluting, collecting 50% ethanol solution eluent, and subjecting the 50% ethanol solution eluent to vacuum concentration to obtain rosmarinic acid crude extract; (3) isolating and purifying by hydrophilic liquid chromatography, to be specific, dissolving the rosmarinic acid crude extract, filtering with a microporous filter membrane, and isolating and purifying by using hydrophilic liquid chromatography to obtain rosmarinic acid having purity of higher than 98%. In addition, the invention also discloses application of the rosmarinic acid. The method is simple and is easy for large-scale production.

Description

technical field [0001] The invention relates to a preparation method and application of a natural active ingredient, in particular to a method for separating and preparing rosmarinic acid from P. Background technique [0002] different leaf green orchid ( Dracocephalum heterophyllum Benth) is a plant of the genus Qinglan in the Lamiaceae family. This plant is widely used in the folks to treat various diseases such as jaundice fever, febrile headache, high blood pressure, lymphadenitis, lung heat cough, and liver heat. Modern research shows that rosmarinic acid has anti-oxidation, anti-virus, anti-bacterial, anti-inflammatory, anti-tumor, immunosuppressive, anti-platelet aggregation and thrombosis, anti-angiogenesis, anti-depression, anti-neurodegenerative diseases, inhibition The proliferation of glomerular mesangial cells, anti-hepatic fibrosis and other pharmacological activities. But so far there is no method for separating and preparing rosmarinic acid monomer from P. ...

Claims

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Application Information

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IPC IPC(8): C07C67/48C07C67/56C07C69/732A61K31/216A61P1/16A61P31/12A61P37/06
CPCA61K31/216C07C67/48C07C67/56C07C69/732
Inventor 王启兰党军陶燕铎王蒲郑威
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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