Rapid source-tracking method of lactobacillus fermenti, combined sequences used for method, and construction method of combined sequences

A technology of Lactobacillus fermentum and construction method, which is applied in the field of rapid traceability of Lactobacillus fermentum, and can solve problems such as low efficiency, inability to ferment Lactobacillus isolate gene fragments, and a large amount of sequence comparison
CN106480187AActive Publication Date: 2017-03-08完美(广东)日用品有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
完美(广东)日用品有限公司
Publication Date
2017-03-08

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Abstract

The invention provides applications of combined gene sequences comprising lactobacillus fermenti genomes in source tracking of lactobacillus fermenti. The combined gene sequences comprise six house-keeping genes, namely, dna A, pyrG, rpoB, recA, dnaK and murC. The invention further provides a rapid source-tracking method of lactobacillus fermenti. The rapid source-tracking method comprises the following steps: (1) extracting genome DNA of lactobacillus fermenti; (2) constructing the combined gene sequences of the bacterial strains; (3) constructing adjacent method phylogenetic trees for the combined gene sequences by adopting MEGA 6.0 software, thus obtaining the source-tracking analysis for the lactobacillus fermenti strains through the phylogenetic trees; and (4) judging the bacterial strains in the same branch in the phylogenetic trees as the same resource. With the method, the bacterial strains with different resources can be effectively and rapidly separated, so that the source tracking for lactobacillus fermenti is realized, and the phylogenetic tree relationship of the lactobacillus fermenti strains with different resources is determined.
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Description

[0001] 【Technical field】

[0002] The invention relates to a rapid traceability method for Lactobacillus fermentum, in particular to a traceability method based on a phylogenetic tree constructed by a joint sequence composed of multiple housekeeping genes.

[0003] 【Background technique】

[0004] Lactobacillus fermentum (Lactobacillus fermentum) belongs to the Lactobacillus genus in the Lactobacillus family, Gram-positive facultative anaerobic bacteria, and does not produce spores. It is the main heterofermentative Lactobacillus in the human intestinal tract. At present, a large number of reports indicate that Lactobacillus fermentum has the functions of hydrolyzing bile salts and lowering cholesterol. It is mostly used in soymilk fermentation and sausage fermentation, can prevent the growth of harmful bacteria, and has an important beneficial effect on the health of the host.

[0005] In recent years, more and more molecular methods have been used to analyze the genetic divers...

Claims

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