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High-efficiency preparation technique of heparin sodium

A preparation process, the technology of heparin sodium, applied in the field of extracting heparin sodium, can solve the problems of increased difficulty in preparation, low product yield, incomplete decomposition, etc., and achieve the goals of short production cycle, high product yield, and improved purity and yield Effect

Inactive Publication Date: 2017-03-15
怀宁县恒达畜产品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current heparin sodium extraction process has a low yield of the product, a relatively large residual amount, incomplete decomposition, many impurities after decomposition, low purity, a large amount of protein, and dark brown color, which increases the difficulty of clinical drug preparation.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] A high-efficiency preparation process for heparin sodium, comprising the following steps:

[0017] (1) Ultrasonic enzymatic hydrolysis of intestinal mucosa: Wash and mince pig intestines, add water and mix to obtain pig intestine mixed water, mix the feed liquid with a pH value of 8 and pig intestine mixed water at a temperature of 45°C with 3% The volume ratio of the protease was hydrolyzed under ultrasonic conditions, the ultrasonic power was 350W, and the hydrolysis time was 45min, then naturally precipitated, and the filtrate was collected by cross-flow filtration through a ceramic membrane;

[0018] (2) Adsorption: adjust the pH value of the filtrate at 7.0, control the temperature of the filtrate at 55°C, adjust the salinity of the filtrate to 2.5°, add Rohm and Haas resin to the filtrate, add 5kg of crude heparin sodium per 100 million international units Rohm and Haas resin, after stirring and absorbing for 5 hours, discharge and collect the resin;

[0019] (3)...

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PUM

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Abstract

The invention discloses a high-efficiency preparation technique of heparin sodium. The technique comprises the following steps: (1) intestinal mucosa ultrasonic enzymolysis; (2) adsorption; resin adsorption; (3) elution; (4) alcohol precipitation: ethanol precipitation; (5) purification; and (6) drying.Impurity removal is carried out after the ultrasonic enzymolysis to obviously enhance the purity and yield of the heparin sodium finished product, so that the heparin sodium has higher purity and high product yield; and the technique lowers the difficulty for further purifying the heparin sodium for patent medicines afterwards, thereby ensuring the final medicinal heparin sodium to have the advantages of high product purity, favorable quality, simple technique and short production cycle.

Description

technical field [0001] The invention relates to the technical field of heparin sodium preparation, in particular to a process for extracting heparin sodium from animal casings. Background technique [0002] Heparin sodium is a sulfate ester of acidic mucopolysaccharide produced by mast cells of animal connective tissue. Because of its strong anticoagulant effect, it is the drug of choice for preventing and treating thromboembolic diseases such as deep venous thrombosis. With the development of research In-depth, people found that heparin sodium not only has anticoagulant, antithrombotic and blood lipid adjustment effects, but also has various biological functions such as anti-inflammation, anti-allergy, anti-virus, and anti-cancer. Although heparin sodium has been used clinically for more than 60 years, so far there is no product that can completely replace it, so it is still the most important anticoagulant and antithrombotic biochemical drug, and currently it can only be o...

Claims

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Application Information

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IPC IPC(8): C08B37/10
CPCC08B37/0075
Inventor 王卫平
Owner 怀宁县恒达畜产品有限公司