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Preparation method of culture substrate and mangosteen cell secondary metabolite

A technology for secondary metabolites and culture medium, which is applied in the field of preparation of culture medium and secondary metabolites of mangosteen cells, can solve the problems of easy residues, affecting the yield of finished products, and low safety.

Inactive Publication Date: 2017-03-15
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Mainly there are following problems: 1. The chemical synthesis method process is complicated
2. Low safety, a large amount of organic solvents are used in the production process, and it is easy to remain in the finished product
3. Limited by the growing season of mangosteen, it affects the output of finished products

Method used

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  • Preparation method of culture substrate and mangosteen cell secondary metabolite

Examples

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preparation example Construction

[0036] The invention provides a method for preparing secondary metabolites of mangosteen cells, comprising the following steps:

[0037] Obtain mangosteen cell lines:

[0038] Take 1kg of fresh mangosteen for cleaning and disinfection, soak in 10L 15%-20% sodium hypochlorite for 20 minutes. Pour off the sodium hypochlorite solution, and wash with 2L of sterile deionized water each time under aseptic conditions, a total of three times, stirring gently during each wash.

[0039] Under aseptic conditions, remove the sterilized mangosteen peel, cut it into pieces with sterilized scissors, and the size of each piece is ≤1mm2.

[0040] Transfer the mangosteen peel fragments to a sterile clean beaker, add 10ml of MS (Murashige and Skoog) medium, 0.01-0.10% cellulase and 0.01-0.10% pectinase per 1.0g of fragments, and digest for 3 hours. Preferably, the volume ratio of cellulase and pectinase is 1:1.

[0041] Filter the digestive juice with a 200-mesh sterile mesh sieve to remove i...

Embodiment 1

[0062] Obtain mangosteen cell lines:

[0063] Take 1kg of fresh mangosteen for cleaning and disinfection, soak in 10L 15%-20% sodium hypochlorite for 20 minutes. Pour off the sodium hypochlorite solution, and wash with 2L of sterile deionized water each time under aseptic conditions, a total of three times, stirring gently during each wash.

[0064] Under aseptic conditions, remove the sterilized mangosteen peel, cut it into pieces with sterilized scissors, and the size of each piece is ≤1mm2.

[0065] Transfer the mangosteen peel fragments to a sterile clean beaker, add 10 ml of MS (Murashige and Skoog) medium, 0.01% cellulase and 0.05% pectinase per 1.0 g of fragments, and digest for 3 hours. The enzyme activity of cellulase is 30000U / mg, and that of pectinase is 50000U / mg.

[0066] Filter the digestive juice with a 200-mesh sterile mesh sieve to remove impurities and obtain a mangosteen cell line.

[0067] Mass culture of mangosteen cells:

[0068] Add No. I sterilized ...

Embodiment 2

[0080] Obtain mangosteen cell lines:

[0081] Take 1kg of fresh mangosteen for cleaning and disinfection, soak in 10L 15%-20% sodium hypochlorite for 20 minutes. Pour off the sodium hypochlorite solution, and wash with 2L of sterile deionized water each time under aseptic conditions, a total of three times, stirring gently during each wash.

[0082] Under aseptic conditions, remove the sterilized mangosteen peel, cut it into pieces with sterilized scissors, and the size of each piece is ≤1mm2.

[0083] Transfer the mangosteen peel fragments to a sterile clean beaker, add 10 ml of MS (Murashige and Skoog) medium, 0.10% cellulase and 0.01% pectinase per 1.0 g of fragments, and digest for 3 hours. The enzyme activity of cellulase is 30000U / mg, and that of pectinase is 50000U / mg.

[0084] Filter the digestive juice with a 200-mesh sterile mesh sieve to remove impurities and obtain a mangosteen cell line.

[0085] Mass culture of mangosteen cells:

[0086] Add No. I sterilized ...

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Abstract

The invention relates to the field of cells, and in particular to a preparation method of a culture substrate and a mangosteen cell secondary metabolite. The culture substrate is prepared from 2,4-D,6-BA and a basal culture medium. The secondary metabolite in the mangosteen cell culture prepared in the culture substrate contains total oxygen anthrone compounds. The secondary metabolite is collected, and dry-frozen for storage. The anti-oxidation function of the total oxygen anthrone compounds is utilized to attain the goal of anti-aging effect, thus enabling the dry-frozen powder to be used in cosmetics.

Description

technical field [0001] The invention relates to the field of cells, in particular to a culture medium and a method for preparing secondary metabolites of mangosteen cells. Background technique [0002] Mangosteen, also known as mangosteen, mangosteen, and phoenix fruit, is the fruit of the evergreen tree mangosteen of the genus Garcinia in the family Guttiferae. In recent years, the pharmacological effects of mangosteen have become a research hotspot. Mangosteen husk contains a variety of xanthone derivatives, mainly including α-mangostin, γ-mangostin and so on. These xanthones exhibit various biological activities, such as anti-inflammatory, anti-tumor, anti-oxidative and antibacterial activities against various bacteria. Biochemically, xanthones are considered to be the only family found in nature where the substitution of tricyclic aromatics by various phenols, methoxyls and isoprenes leads to a series of derivatives. Currently, researchers have identified and isolated...

Claims

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Application Information

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IPC IPC(8): C12N5/04C12P17/06A23L33/105A61K8/49A61K31/352C07D311/86A61P39/06A61Q19/08
CPCA23V2002/00A61K8/498A61K31/352A61Q19/08C07D311/86C12N5/0025C12N2500/30C12N2500/34C12N2500/40C12P17/06A23V2200/302A23V2250/21
Inventor 陈海佳葛啸虎王一飞吴子杰王小燕
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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