Method for preparation of composite microbial paraffin removal and inhibition agent
A technology for removing wax and preventing microorganisms, which is applied to the methods of using microorganisms, chemical instruments and methods, compositions for drilling holes, etc., can solve the problems of poor wax removal and preventing effect, etc., and achieves good effects, simple preparation steps and low cost. Effect
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example 1
[0018] First weigh 30g of salted fish and add it to the fermenter, then add a 15% sodium chloride solution to submerge the salted fish, and ferment it at 45°C for 6 days. After the fermentation is over, collect the fermented liquid and filter it. Halobacteria liquid, for later use; weigh 30g of glucose, 15g of yeast extract, 8g of sodium chloride and 1000mL of deionized water, add them to a petri dish, stir and mix, transfer to a sterilizing tank, and sterilize at 105°C for 10 minutes. Prepare the culture medium, then take the biogas liquid for filtration, collect the filtrate, take 200mL of the filtrate and add it to the culture medium, and place it in a shaker, cultivate it at 150r / min, 85°C for 3h, remove the bacteria, and then lower the temperature to 75°C ℃, cultured for 3 days, after the culture was completed, collected the culture medium and placed it in a centrifuge, centrifuged at 1000r / min for 30min, collected the strains of the lower layer, and obtained the strains o...
example 2
[0021] First weigh 20g of salted fish and add it to the fermenter, then add a 15% sodium chloride solution to submerge the salted fish, and ferment for 5 days at 35°C. After the fermentation is over, collect the fermentation broth and filter it, and collect the filtrate to obtain Halobacteria liquid, for later use; weigh 20g of glucose, 10g of yeast extract, 5g of sodium chloride and 800mL of deionized water, add them to a petri dish, stir and mix, transfer to a sterilizing tank, and sterilize at 100°C for 5 minutes. Prepare the culture medium, then take the biogas liquid for filtration, collect the filtrate, take 100mL of the filtrate and add it to the culture medium, and place it in a shaker, and cultivate it at 130r / min, 80°C for 2h to remove miscellaneous bacteria, and then lower the temperature to 70°C Cultivate for 2 days at ℃. After the cultivation is over, collect the culture medium and put it in a centrifuge, centrifuge at 800r / min for 20min to collect the bacteria in ...
example 3
[0024] First weigh 25g of salted fish and add it to the fermenter, then add 15% sodium chloride solution to submerge the salted fish, and ferment for 5 days at 37°C. Halophilic bacteria liquid, for later use; weigh 25g glucose, 12g yeast extract, 7g sodium chloride and 900mL deionized water, add to a petri dish, stir and mix, transfer to a sterilizing tank, and sterilize at 102°C for 7 minutes, Prepare the culture medium, then take the biogas liquid for filtration, collect the filtrate, take 150mL of the filtrate and add it to the culture medium, put it in a shaker, and cultivate it at 140r / min, 82°C for 2h to remove miscellaneous bacteria, and then lower the temperature to 72°C ℃, culture for 2 days, after the culture is over, collect the culture medium and put it in a centrifuge, centrifuge at 900r / min for 25min, collect the bacteria in the lower layer, and get the thermophilic bacteria; then weigh 12g of beef extract and 12g of glucose , 25g of paraffin, and 250mL of deioni...
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