A kind of preparation method of soybean Dan peptide chelated calcium

A technology for chelating calcium and soybean Dan peptide, which is applied to the preparation method of peptides, chemical instruments and methods, peptides, etc., can solve the problems of decreased calcium bioavailability and unsatisfactory calcium supplementation effect, and achieve the goal of alleviating the national calcium deficiency The effects of pressure, improving efficiency and pertinence, and enhancing overall competitiveness

Active Publication Date: 2020-04-07
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, there are more and more calcium supplement products, and the annual sales are also increasing year by year, but the effect of calcium supplementation is not ideal. Forms insoluble calcium salts, resulting in decreased calcium bioavailability

Method used

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  • A kind of preparation method of soybean Dan peptide chelated calcium
  • A kind of preparation method of soybean Dan peptide chelated calcium
  • A kind of preparation method of soybean Dan peptide chelated calcium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Using supercritical CO 2 Extraction and degreasing method is used to degrease soybean Dan, the extraction temperature is 40°C, CO 2 The flow rate is 25L / h, the pressure is 35Mpa, and the extraction time is 1.5h. Superfinely pulverize the defatted soy bean, mix according to the mass ratio of soy bean and water of 1:10, adjust to pH 7.5, temperature 40°C, add alkaline protease with 0.5% mass of soy bean to enzymatically hydrolyze the soy bean 12h, the enzymolysis solution was stirred at a speed of 40 rpm. After the enzymatic hydrolysis, the enzymatic hydrolysis solution was heated to 75°C for 20 minutes to inactivate the protease. The enzymatic solution was centrifuged at 10,000 rpm for 20 minutes, and the supernatant was taken. The supernatant was removed by an ultrafiltration membrane with a molecular weight cut-off of 300kD to obtain a purified liquid. The purified solution is separated and purified by ultrafiltration membranes with molecular weight cut-offs of 5kD...

Embodiment 2

[0070] Using supercritical CO 2 The extraction and degreasing method is used to degrease soybean Dan, the extraction temperature is 45°C, CO 2 The flow rate is 15L / h, the pressure is 20Mpa, and the extraction time is 3h. Superfinely pulverize the defatted soy bean, mix according to the ratio of soy bean and water mass ratio of 1:15, adjust to pH4, temperature 30°C, add 0.3% neutral protease of soy bean mass to enzymatically hydrolyze the soy bean for 20 hours . The enzymatic solution was stirred at a speed of 50 rpm. After the enzymatic hydrolysis, heat the enzymatic hydrolysis solution to 80°C for 10 minutes to inactivate the protease. The enzymatic solution was centrifuged in a centrifuge at 8000 rpm for 30 minutes, and the supernatant was taken. The supernatant was removed by an ultrafiltration membrane with a molecular weight cut-off of 200kD to obtain a purified liquid. The purified solution is separated and purified by ultrafiltration membranes with molecular weight...

Embodiment 3

[0072] Using supercritical CO 2 The extraction and degreasing method is used to degrease soybean Dan, the extraction temperature is 45°C, CO 2The flow rate is 15L / h, the pressure is 10Mpa, and the extraction time is 1-4h. Superfinely pulverize the defatted soy bean, mix evenly according to the ratio of soy bean and water mass ratio of 1:20, adjust to pH8, temperature 20°C, add a compound enzyme of trypsin and alkaline protease with 1% of bean mass to treat the bean. Dan was subjected to enzymatic hydrolysis for 18 hours, wherein the weight ratio of trypsin and alkaline protease was 1:2. The enzymatic solution was stirred at a speed of 25 rpm. After the enzymatic hydrolysis, heat the enzymatic hydrolysis solution to 85°C for 10 minutes to inactivate the protease. The enzymatic solution was centrifuged in a centrifuge at 12,000 rpm for 10 minutes, and the supernatant was taken. Use an ultrafiltration membrane with a molecular weight cut-off of 250Kd to remove impurities with...

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Abstract

The invention discloses a preparation method for peptide chelated calcium of clanis bilineata tsingtauica larva. The preparation method comprises the following steps: degreasing the clanis bilineata tsingtauica larva and then carrying out superfine crushing, adding water and uniformly mixing, and adjusting the pH value to 2-9; adding protease in the obtained mixed liquid, stirring for enzymatic hydrolysis, then inactivating the protease to obtain a crude enzymatic hydrolysate, and centrifuging to obtain an supernatant liquid; taking the obtained supernatant liquid and removing impurities to obtain a purified liquid, and carrying out separation and purification by virtue of at least one ultrafiltration membrane with a molecular weight cut-off of 1-50kD to obtain enzymatic hydtolysis peptide components with different molecular weights; adding a calcium source, and chelating to obtain a crude peptide chelated calcium product of the clanis bilineata tsingtauica larva; and collecting precipitates after concentration and precipitation, and drying to obtain the peptide chelated calcium product of the clanis bilineata tsingtauica larva. According to the method disclosed by the invention, a method of classifying through the ultrafiltration membranes with different molecular weight cut-off at first, and then chelating is adopted for the enzymatic product, on one hand, the technical bottleneck that a peptide calcium chelate is difficult to separate is solved; and on the other hand, the peptide chelated calcium products with different molecular weights of the clanis bilineata tsingtauica larva are prepared and can be used by people with different needs, thus the use efficiency of the products is increased, and the pertinence of the products is improved.

Description

technical field [0001] The invention belongs to the field of deep processing of agricultural products, in particular to a preparation method of soybean Dan peptide chelated calcium. Background technique [0002] Peptide calcium chelate is formed through the coordination of peptide N-terminal amino group, C-terminal carboxyl group, amino acid side chain, and carbonyl and imino groups in the peptide chain with calcium ions. It has the advantages of easy absorption, good nutritional value, and high biological potency. , and many biological activities such as antibacterial, anti-oxidation, hypoglycemic and lipid-lowering, immune regulation, etc. Compared with the peptide calcium chelate derived from plants, the animal peptide calcium chelate has the characteristics of easy absorption and high nutritional value. In the past, livestock and poultry animal tissues were the main source of animal-derived active peptides. However, due to the occurrence of diseases such as mad cow dis...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K1/107A23J3/04A23J3/34A23L33/165A23L33/18
CPCA23J3/04A23J3/341A23V2002/00C07K1/107C12P21/06A23V2200/30A23V2200/328A23V2200/324Y02P20/54
Inventor 夏秀东周剑忠单成俊刘小莉王英李莹张丽霞黄自苏
Owner JIANGSU ACAD OF AGRI SCI
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