Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

A fusion protein vaccine that inhibits streptococcal and/or prevents streptococcal infection

A fusion protein and protein technology, applied in the direction of bacteria, fungi, hybrid peptides, etc., can solve problems that hinder the development of type A streptococcal vaccines

Active Publication Date: 2019-12-31
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Due to the large number of serotypes of Streptococcus A and the fact that they can cause autoimmune diseases, the development of Streptococcus A vaccines has been seriously hindered. As a result, there is currently no vaccine against this bacteria available in the world. Therefore, the development of safe, effective, broad-spectrum A Strep A vaccine is urgently needed

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A fusion protein vaccine that inhibits streptococcal and/or prevents streptococcal infection
  • A fusion protein vaccine that inhibits streptococcal and/or prevents streptococcal infection
  • A fusion protein vaccine that inhibits streptococcal and/or prevents streptococcal infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0114] Embodiment 1, the construction of CTB-Sortase A protein gene and the preparation of CTB-Sortase A protein thereof

[0115]1. Replace the DNA fragment between the NcoI and BamHI recognition sequences of the vector pET26b (+) with the DNA sequence shown in the 112-996th position of SEQ ID No.2, keep other DNA sequences unchanged, and obtain the recombinant expression vector pET26b- CTB-Sortase A. Sequencing results show that the recombinant expression vector pET26b-CTB-Sortase A is inserted into the DNA sequence shown in the 112th-996th position of SEQ ID No.2, and the recombinant expression vector pET26b-CTB-Sortase A can express the first sequence of SEQ ID No.1. The CTB-Sortase A protein shown in the 23-314 position, the DNA sequence shown in the 115-996 position of SEQ ID No.2 is the encoding of the CTB-Sortase A protein shown in the 23-314 position of SEQ ID No.1 sequence.

[0116] 2. Transform the recombinant vector pET26b-CTB-Sortase A into BL21(DE3)plysS compete...

Embodiment 2

[0124] Example 2. Immunoprotection against Type A Streptococcus infection after nasal cavity inhalation of CTB-Sortase A protein

[0125] Female BALB / c mice aged 6-8 weeks were randomly divided into three groups, with 6 mice in each group, and the grouping process was as follows:

[0126] PBS group: On the 1st day, 5th day, 10th day and 14th day of the experiment, 10 μL of PBS buffer was instilled through the nasal cavity;

[0127] CTB-Sortase A protein 5 μg group: On the 1st day, the 5th day, the 10th day and the 14th day of the experiment, 10 μL CTB-Sortase A protein solution (vaccine solution) was instilled through the nasal cavity respectively, and each dose of these four times 5 μg CTB-Sortase A protein;

[0128] CTB-Sortase A protein 10 μg group: On the 1st day, the 5th day, the 10th day and the 14th day of the experiment, 10 μL CTB-Sortase A protein solution (vaccine solution) was instilled through the nasal cavity respectively. 10 μg CTB-Sortase A protein;

[0129] ...

Embodiment 3

[0131] Example 3. Immunoprotection against Type A Streptococcus infection after nasal cavity inhalation of different doses of CTB-Sortase A protein

[0132] Female BALB / c mice aged 6-8 weeks were randomly divided into four groups, with 6 mice in each group, and the grouping process was as follows:

[0133] PBS group: On the 1st day, 5th day, 10th day and 14th day of the experiment, 10 μL of PBS buffer was instilled through the nasal cavity;

[0134] CTB-Sortase A protein 1 μg group: On the 1st day, the 5th day, the 10th day and the 14th day of the experiment, 10 μL of CTB-Sortase A protein solution (vaccine solution) was instilled through the nasal cavity respectively. 1 μg CTB-Sortase A protein;

[0135] CTB-Sortase A protein 2.5 μg group: 10 μL CTB-Sortase A protein solution (vaccine solution) was instilled through the nasal cavity on the 1st, 5th, 10th and 14th days of the experiment. Both are 2.5 μg CTB-Sortase A protein;

[0136] CTB-Sortase A protein 5 μg group: On th...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
molecular weightaaaaaaaaaa
Login to View More

Abstract

The invention discloses a fusion protein vaccine capable of inhibiting Streptococcus and / or preventing Streptococcus infection. With fusion protein obtained through linkage of sortase A and a cholera toxin B subunit through connecting peptide, the Th17 cell activation level is remarkably increased, the effects of preventing pathogenic bacteria from settling and quickly removing the pathogenic bacteria can be realized, and the fusion protein has protecting effects on Group A Streptococcus of different serotypes and has the superiority of high efficiency, broad spectrum and low cost. With the fusion protein, the use amount of immunogen is reduced, the production technology is simplified, and the production cost is reduced. Meanwhile, the vaccine adopts the way of mucosal immunity, has the characteristics of being free of tissue damage, free of local side effects and convenient to use, and is easy to popularize and use.

Description

technical field [0001] The invention relates to a fusion protein vaccine for inhibiting streptococcus and / or preventing streptococcal infection in the field of biotechnology. Background technique [0002] Group A Streptococcus (Group A Streptococcus pyogenes) is a common Gram-positive opportunistic pathogenic bacteria in clinical practice, which can cause a variety of diseases, including pharyngitis, impetigo, erysipelas and cellulitis in mild cases, and severe cases can Life-threatening, such as pneumonia, bacteremia, toxic shock, and acute necrotizing fasciitis [1] . According to statistics, at least 517,000 people die from type A streptococcal infection every year in the world, most of them die from rheumatic fever and rheumatic heart disease caused by type A streptococcal infection, and about 163,000 people die from acute type A streptococcal infection. Infect [2] . [0003] There are more than 150 serotypes of Streptococcus A, and new serotypes are constantly emergi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07K19/00C12N15/62C12N15/63C12N1/15C12N1/19C12N1/21C12N1/13A61K39/09A61K39/00A61P31/04
Inventor 王北难毕帅
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com