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Enzymatic deacidifying method for high-acid-value oil

A technology of high-acid-value oil and deacidification method, which is applied in the field of high-acid-value oil and fat enzymatic deacidification, can solve problems such as reducing the recovery rate of neutral oil, and achieve the effects of high recovery rate, mild reaction conditions and high removal rate

Active Publication Date: 2017-04-19
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing enzymatic deacidification technology of high-acid oil generally uses lipase as a catalyst. During the deacidification process, there will be side reactions such as hydrolysis and glycerolysis of neutral oils (glycerides), which reduces the neutral oils. recovery rate

Method used

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  • Enzymatic deacidifying method for high-acid-value oil
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  • Enzymatic deacidifying method for high-acid-value oil

Examples

Experimental program
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Effect test

Embodiment 1

[0021] Embodiment 1 (mutant LipaseSMG1)

[0022] Get 20g degummed, decolorized rice bran oil, the free fatty acid content of rice bran oil is 25.6%, and the glycerin of 3.42g is placed in the 50mL conical flask with stopper (the mol ratio of free fatty acid and glycerin is 1:2), mix and pre- After heating to 30°C, add 0.5g of partial glyceride lipase Lipase SMG1Phe278Asp, start deacidification reaction at 30°C, magnetic stirring speed 400r / min, centrifuge the reaction mixture after esterification reaction for 6h, recover glycerol phase and oil respectively phase, the oil phase is deacidified oil. Using liquid chromatography to analyze the free fatty acid content of the sample, the free fatty acid content of rice bran oil decreased from the initial 25.6% to 1.42%, while the triglyceride content in rice bran oil remained unchanged; the removal rate of free fatty acid can reach 94.4%.

Embodiment 2

[0023] Embodiment 2 (mutant LipaseSMG1)

[0024] Get 20g degummed, decolorized rice bran oil, the free fatty acid content of rice bran oil is 25.6%, and the glycerol of 1.71g is placed in 50mL tool stopper conical flask (the mol ratio of free fatty acid and glycerin is 1:1), mix and pre- After heating to 30°C, add 0.5g of partial glyceride lipase Lipase SMG1Phe278Asp, start deacidification reaction at 30°C, magnetic stirring speed 400r / min, centrifuge the reaction mixture after esterification reaction for 6h, recover glycerol phase and oil respectively phase, the oil phase is deacidified oil. Using liquid chromatography to analyze the free fatty acid content of the sample, the free fatty acid content of rice bran oil decreased from the initial 25.6% to 2.41%, while the triglyceride content in rice bran oil remained unchanged; the removal rate of free fatty acid can reach 90.6%.

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Abstract

The invention discloses an enzymatic deacidifying method for high-acid-value oil, belonging to the field of biochemical industry. The method comprises the following steps: (1) mixing the high-acid-value oil with glycerol, and with a mutant of partial glyceride lipase SMG1 as a catalyst, subjecting the glycerol and free fatty acid in the high-acid-value oil to an esterification reaction, wherein the amino acid sequence of the mutant is shown as in SEQ NO.1; and (2) separating a reaction product, and recycling an oil phase so as to obtain enzymatic deacidified oil. According to the invention, the glycerol is utilized as an acyl acceptor of the fatty acid, so the removal rate of the free fatty acid can reach 90% or above; neutral oil is free of side reactions; meanwhile, the reaction product is easy to separate and purify.

Description

technical field [0001] The invention belongs to the field of biochemical industry and relates to an enzymatic deacidification method of high acid value oil. Background technique [0002] Normal production of animal and vegetable oils generally has a relatively low free fatty acid content, but under special circumstances, such as high moisture in the oil, improper storage, and enzyme action during processing, etc., the acid value of the produced oil is relatively high, among which the typical Vegetable oils include high acid value wheat germ oil and high acid value rice bran oil, and high acid value animal oils include high acid value tuna oil and high acid value squid oil. The acid value of oil with high acid value is generally above 20mgKOH / g. Difficulty in processing oil with high acid value is a common problem in the oil industry. The existing processing technology of oil with high acid value consumes a lot of energy, which brings great harm to environmental governance an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11C3/06C12P7/64
CPCC11C3/06C12P7/6454
Inventor 王永华李道明王卫飞李雪辉杨博蓝东明严慧玲
Owner SOUTH CHINA UNIV OF TECH