Immunocolloidal gold test card of methomyl and preparation method thereof

A technology of colloidal gold and methomyl, applied to measuring devices, instruments, scientific instruments, etc., to achieve simple methods and accurate results

Inactive Publication Date: 2017-04-19
DANYANG YITAI BIOTECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the above situation, the purpose of the present invention is to provide a methomyl immune colloidal gold detection card and a preparation method thereof in order to overcome the defects in the prior art, which can effectively solve the problem that methomyl can be detected quickly and easily

Method used

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  • Immunocolloidal gold test card of methomyl and preparation method thereof
  • Immunocolloidal gold test card of methomyl and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0020] figure 1 , figure 2 Shown embodiment: among the figure 9 is a PVC rubber sheet; 7 is a sample pad; 8 is a colloidal gold binding pad, and the colloidal gold binding pad is coated with a monoclonal antibody colloidal gold marker; 10 is a coating film, i.e. nitrocellulose Plain membrane, the nitrocellulose membrane is coated with methomyl-BSA and goat anti-mouse IgG; 11 is a water-absorbent pad, made of water-absorbent materials such as filter paper.

[0021] On one end (sample end) of the PVC rubber plate 9, the sample pad 7 and the bonding pad 8 are adhered, and the sample pad 7 and the bonding pad 8 are in a side-by-side structure.

[0022] A nitrocellulose membrane 10 is adhered in the middle of the PVC rubber sheet 9 . Goat anti-mouse IgG quality control line 3 and methomyl-BSA detection line 2 are set on the nitrocellulose membrane 10 .

[0023] At the other end of the PVC rubber sheet 9, a water-absorbing pad 11 is adhered. One end of the nitrocellulose membra...

Embodiment 2

[0025] The preparation of methomyl colloidal gold detection card is realized by the following steps:

[0026] Preparation of colloidal gold solution: Take a 1 L Erlenmeyer flask, add 495 mL of ultrapure water, and then add 1% chloroauric acid (HAuCl 4 ·3H 2 (0) 5 mL, prepared into 500 mL 0.01% chloroauric acid aqueous solution, heated and boiled, then added 1% trisodium citrate (Na 3 C 6 h 5 o 7 2H 2 O) Solution 5-7 mL, continue to stir and heat, when the color of the solution turns completely transparent purple red, stop heating after maintaining for 5 min, add water to the original volume, cool to room temperature, and store at 2-8°C for later use;

[0027] Antibody pretreatment: Centrifuge the methomyl antibody to be labeled at 1000 r / min, 4°C for 20 min, take the supernatant, and dilute it with 0.01 mol / L PBS to 1 mg / mL; or use 0.01 mol / L Dilute to 1 mg / ml in PBS and pass through a 0.22 µm filter;

[0028] Preparation of colloidal gold markers: Take 40 mL of the col...

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Abstract

The invention discloses an immunocolloidal gold test card of methomyl and a preparation method thereof, and relates to the technical field of veterinary drug residue detection of animal-derived food. A test strip in the shell of the test card is composed of a PVC rubber sheet, a sample pat, a colloidal gold conjugate pad, a coating membrane and an absorbent pad. The colloidal gold membrane is a glassfiber membrane containing a methomyl monoclonal antibody. The coating membrane is a nitrocellulose membrane, on which a T line and a C line are arranged. The T line is coated with a methomyl protein conjugate, and the C line is coated with a goat anti-mouse IgG antibody. The test card of the invention is effectively used for rapid detection of methomyl, is convenient and fast, and has accurate results.

Description

technical field [0001] The invention relates to the technical field of pesticide residue detection in cereal foods, in particular to an immune colloidal gold detection card for methomyl and a preparation method thereof. Background technique [0002] Methomyl is a broad-spectrum carbamate insecticide with strong volatility and high inhalation toxicity. It is mainly used to control pests such as Chilo borer, Planthopper and Spodoptera litura. The original drug is white crystal powder, and the commercial state is wettable powder, soluble powder or emulsifiable concentrate. Methomyl is a broad-spectrum carbamate insecticide with systemic contact and stomach toxicity. First recommended as an insecticide and nematocide. It is suitable for controlling aphids, moths, cutworms, etc. in cotton, tobacco, fruit trees, and vegetables. Therefore, regular detection of methomyl residues has become a necessary means of monitoring in many countries. [0003] Existing technologies mainly u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577
CPCG01N33/577
Inventor 洪霞杜霞
Owner DANYANG YITAI BIOTECH DEV
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