Pneumocandin B0 purification method

Abstract

The present invention discloses a process-optimizing pneumocandin B0 purification method, which comprises: (1) acidifying a pneumocandin B0 fermentation broth, filtering, and leaching to obtain a leaching liquid; (2) adding diatomite coating crystal to the leaching liquid concentration mixture, adding deionized water, and centrifugating to obtain a white solid containing pneumocandin B0; (3) adding ethanol to the obtained solid, dissolving, completely stirring, adding active carbon, completely stirring and decolorizing, and filtering to obtain a colorless filtrate; (4) concentrating the colorless filtrate to achieve a dry state, adding methanol to dissolve the dried filtrate, adding water, stirring to achieve a dissolved clarification state, adding ethyl acetate, stirring, and crystallizing to obtain a primary wet crystal; and (5) crystallizing the wet crystal again by using the same crystallizing method to obtain a wet crystal, and drying. With the method of the present invention, on the basis of the unchanged purity, the process steps are simplified, the production cost is reduced, and the industrialized mass production is easily achieved.

Description

technical field [0001] The invention belongs to the field of medicinal chemistry and relates to pneumocantine B 0 The industrialized production of, be specifically related to a kind of purification pneumocidine B 0 industrial methods. Background technique [0002] Neomercontin B 0 It is a secondary metabolite synthesized by the fermentation of the fungus Glarea lozoyensis. In addition to the target product, the fermentation product of Glarealozoyensis has C 0 and other components, as well as other impurities such as a large amount of pigment, so Newmocontin B 0 The extraction and purification process is difficult. [0003] [0004] Neomercontin B 0 Cas: 135575-42-7 [0005] [0006] Neomercontin C 0 Cas: 144074-96-4 [0007] Chinese invention patent 200910133118.0 discloses the preparation of pneumocandine B 0 The method, the main steps are: a) centrifugal nemocontin B 0 Fermentation broth, take the mycelia, and extract neomocontin B with methanol 0 ; b) ...

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Problems solved by technology

This method was developed by Borui Biopharmaceutical Taixing Co., Ltd., a subsidiary of the parent company of the applicant company. However, during the process handover, the inventor found that although this method can effectively control C 0 content, making pneumocantine B 0 The purity can reach more than 99%, but in the whole process, column chromatography is required. When column chromatography is applied to industrial production, it is time-consuming, the amount of solvent used is large, it is easy to cause environmental pollution, and the loss of raw materials is large, which greatly increases the production cost.

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