Preparation method of high-titer heparin sodium crude product and production apparatus therefor

Abstract

The invention belongs to the technical field of biology and particularly relates to a preparation method of a high-titer heparin sodium crude product and a production apparatus therefor. The invention provides a reagent composition for preparing the heparin sodium crude product. The reagent composition includes: a low-salt washing reagent A: a NaCl water solution being 2-5% in mass volume percentage; a medium-salt washing reagent B: a NaCl water solution being 3-6% in mass volume percentage; a high-salt washing reagent C: a NaCl water solution being 4-7% in mass volume percentage; an elution reagent D: a NaCl water solution being 8-20% in mass volume percentage; and a precipitation reagent E: a heparin sodium ethanol water solution in 20-40 degrees. In the invention, gradient washing is carried out with the washing reagents in different salinity. The heparin sodium crude product prepared through the method of low-salinity elution and low-ethanol-degree precipitation is low in impurity content and has complete heparin sodium molecular structure. The bioactivity of the heparin sodium crude product is approximate to or reaches injection grade of heparin sodium.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for preparing high-potency crude heparin sodium and a production device thereof. Background technique [0002] Heparin Sodium (Heparin Sodium) Heparin Sodium is a mucopolysaccharide sulfate anticoagulant. Heparin sodium is the sodium salt of aminodextran sulfate extracted from the intestinal mucosa of pigs or cattle, and belongs to mucopolysaccharides. In animals, it mostly exists in the form of heparin sodium-protein complex. In medicine, heparin sodium has an anticoagulant effect both in vivo and in vitro, and can prolong the coagulation time, prothrombin time and thrombin time. Clinically, it has the effects of anticoagulation, lowering blood fat, protecting endothelial cells, anti-platelet accumulation and release, promoting fibrinolysis, inhibiting the proliferation of arterial smooth muscle cells, reducing blood viscosity, lowering blood lipids and anti-i...

AI Technical Summary

Problems solved by technology

Conventional production methods use saturated brine to cause a large amount of waste of salt and environmental pollution, and the crude heparin sodium obtained contains a large amount of protein nucleic acid impurities, organic impurities such as dermatan sulfate and chondroitin sulfate, and has low biological activity.

Images