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A preparation method and fluorescent probe of nanometer fluorescent probe for detecting active oxides

A nano-fluorescent probe and active oxide technology, applied in the direction of fluorescence/phosphorescence, chemical instruments and methods, luminescent materials, etc., can solve the problems of complex preparation steps, and achieve simple preparation methods, good stability, and high detection sensitivity Effect

Inactive Publication Date: 2019-11-05
CHINA PHARM UNIV
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  • Claims
  • Application Information

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Problems solved by technology

At the same time, because the preparation steps of fluorescent probes in the prior art are relatively complicated, involving more reaction equipment, stricter requirements on reaction condition parameters, and toxic reagents may be used, so how to adopt a more concise and easy-to-control method Methods to obtain highly selective and sensitive ROS fluorescent probes will be a research hotspot for those skilled in the art

Method used

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  • A preparation method and fluorescent probe of nanometer fluorescent probe for detecting active oxides
  • A preparation method and fluorescent probe of nanometer fluorescent probe for detecting active oxides
  • A preparation method and fluorescent probe of nanometer fluorescent probe for detecting active oxides

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Embodiment 1

[0039] Embodiment 1: A kind of preparation method of the nanometer fluorescent probe that is used to detect reactive oxide

[0040] Dissolve 0.0400g (0.1mmol) NaOH in 10mL ultrapure water, and then 3CdSO 4 ·8H 20.0770g (0.1mmol), ligand 2,2'-dithiosalicylic acid 0.0926g (0.3mmol), auxiliary ligand 1,10-phenanthroline 0.0198g (0.1mmol) added in the above-mentioned NaOH solution, After mixing, seal the reactor immediately, put it into an oven, heat at 150°C, and react for 24 hours. After the reaction, the reaction kettle was cooled to room temperature, washed with water and ethanol three times respectively, and then centrifuged at 8000rpm to obtain disulfide coordination polymer nanorods, the morphology of which was shown in the attached figure 1 shown. The excitation and emission spectra of disulfide coordination polymer nanorods are shown in the appendix figure 2 .

Embodiment 2

[0041] Embodiment 2: A kind of preparation method of the nanometer fluorescent probe that is used to detect active oxide

[0042] Dissolve 0.0400g (0.1mmol) NaOH in 10mL of ultrapure water, then R6G 0.0100g (0.02mmol), 3CdSO 4 ·8H 2 O 0.0770g (0.1mmol), ligand 2,2'-dithiosalicylic acid 0.0926g (0.3mmol), auxiliary ligand 1,10-phenanthroline 0.0198g (0.1mmol) was added in the above-mentioned NaOH solution, After mixing, seal the reactor immediately, put it into an oven, heat at 150°C, and react for 24 hours. After the reaction, the reactor was cooled to room temperature, washed with water and ethanol for 3 times, and centrifuged at 8000rpm to obtain rhodamine 6G-doped disulfide coordination polymer nanorods. image 3 shown. The abscissa is the wavelength (nm), and the ordinate is the change of fluorescence quenching intensity with time.

Embodiment 3

[0043] Embodiment 3: Use the fluorescent probe prepared in embodiment 1 to carry out the experiment of the fluorescence response to ROS

[0044] Disulfide coordination polymer nanorods (~10 μg·mL -1 ) was suspended in 10mM phosphate buffer (pH 7.4), and then added H 2 o 2 Solution, under the excitation condition of 400nm wavelength, observe the change of fluorescence intensity, the obtained fluorescence spectrum is as attached Figure 4 shown. Disulfide coordination polymer nanorods with H 2 o 2 After the reaction, the morphology changes as attached Figure 5 .

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Abstract

The invention discloses a preparation method of a nano-fluorescence probe for detecting ROS (reactive oxygen species) and the fluorescence probe, and mainly relates to the technical field of reactive oxygen species fluorescence detection. 2, 2'-dithio-salicylic acid serves as a ligand, 1, 10-phenanthroline serves as an auxiliary ligand, and polymerization reaction is performed by taking cadmium ions as central metal ions to obtain a nano-scale rodlike disulfide coordination polymer. The preparation method has the advantages that the preparation method is simple, toxic organic solvents are omitted in the preparation process, strict synthesis conditions are omitted, recognition of the ROS is based on response of disulfide bonds in the ligand to the ROS, good selectivity for the ROS is achieved, and other inorganic ions and organic molecules have no obvious effects on fluorescence signals of the nano-fluorescence probe.

Description

technical field [0001] The invention relates to the technical field of fluorescent detection of active oxides, in particular to a method for preparing a nanometer fluorescent probe for detecting active oxides and the fluorescent probe. Background technique [0002] Reactive Oxygen Species (ROS) widely exist in organisms, including not only some free radicals, such as superoxide anion (O 2 - ), hydroxyl radical ( OH), peroxyl radical ( HO 2 ), peroxy radicals (ROO·) and alkoxyl radicals (RO·), also include some non-free radicals, such as hypochlorous acid (HOCl), singlet oxygen ( 1 o 2 ) and hydrogen peroxide (H 2 o 2 )Wait. ROS participates in the process of human physiological activities and has important physiological significance, but excessive ROS can cause damage to the human body and lead to diseases. Therefore, it is of great scientific significance to achieve highly sensitive detection of ROS. [0003] At present, the methods for detecting ROS mainly include ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08G83/00G01N21/64C09K11/06
CPCC08G83/008C09K11/06C09K2211/188G01N21/643G01N2021/6432
Inventor 王怀松袁冬常裕祥
Owner CHINA PHARM UNIV
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