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Method for rapidly measuring fusarium toxins in cereal grains

A Fusarium mold, rapid detection technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of food safety risks, early warning and evaluation work without scholars, and achieve the effect of controlling grain quality, high sensitivity, and high resolution

Inactive Publication Date: 2017-04-26
南京市食品药品监督检验院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no public detection method for this toxin in China, and no scholars have carried out food safety risk early warning and assessment work on the emerging Fusarium mycotoxins in domestically produced food crops and their deep-processed products

Method used

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  • Method for rapidly measuring fusarium toxins in cereal grains
  • Method for rapidly measuring fusarium toxins in cereal grains

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0032] 1. Sample extraction and purification. Accurately weigh 5g of the homogenized sample into a 50mL centrifuge tube, add 30mL of acetonitrile, extract in an ultrasonic extractor at 250W power for 2min, then add 1g of NaCl, 0.5g of C 18 Powder, tighten the lid, shake up and down 30 times, take it out and centrifuge at 5000r / min for 10min in a centrifuge, take the supernatant and pass it through a 0.22μm filter membrane and test it on the machine;

[0033] 2. Chromatography and mass spectrometry conditions

[0034] Chromatographic conditions

[0035] The liquid chromatography column is Agilent XDB C 18 Column (2.1×50mm, 1.8mm), column temperature 35°C, injection volume 1μL, mobile phase A is water, mobile phase B is acetonitrile, flow rate 0.3mL / min, mobile phase gradient elution program: B within 5min Increase linearly from 20% to 80%, then return to 20% after 1min, and keep for 2min; see the attached sample test spectrum figure 1 .

[0036] Mass Spectrometry Condition...

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Abstract

The invention belongs to the technical field of food detection and particularly relates to a method for rapidly measuring fusarium toxins in cereal grains with a liquid chromatography-tandem mass spectrometry method. Five toxins are enniatin A, enniatin A1, enniatin B, enniatin B1 and beauvericinn respectively. A sample is extracted with an MAE-QuEChERS (microwave-assisted extraction-quick, easy, cheap, rugged, safe) dispersive solid-phase extraction technology without solid-phase extraction column purification and concentration, and the cost can be effectively saved. An Agilent XDB C18 chromatographic column is used for separation, acetonitrile and a water solution are taken as mobile phases for gradient elution, an MRM (multiple reaction monitoring) positive ion mode is adopted for detection, and an external standard method is used for quantification. The method is rapid, accurate, effective and high in operability, can be popularized and used as a powerful means of food safety supervision and management and can be applied to food quality safety detection or entry-exit inspection and quarantine.

Description

[0001] 1. Technical field [0002] The invention belongs to the technical field of food detection, and in particular relates to a method for quickly detecting Fusarium mycotoxin in grains by using liquid chromatography tandem mass spectrometry. [0003] 2. Background technology [0004] Mycotoxins are a class of toxic substances composed of secondary metabolites of filamentous fungi. Due to their toxicity to the human body and their frequent detection in food and plant seeds in recent years, they have attracted worldwide attention. Fusarium, ergot, Aspergillus, Penicillium and Alternaria may produce mycotoxins during crop growth and subsequent storage and transportation, among which the toxins produced by Fusarium are the most popular, and are widely used in agricultural products in America, Europe and Asia prevalent in. [0005] Fusarium molds contain at least 100 species of plant pathogens. Most of the toxins they produce are chemically stable and tend to remain in the final...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 胡文彦杨军孙小杰王玉梅刘新梅
Owner 南京市食品药品监督检验院