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A kind of in vitro rapid propagation method of water tower flower

A water tower flower and fast technology, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve problems such as quality and yield decline, planting benefit decline, and serious virus accumulation, so as to improve yield and quality, and increase load capacity. The effect of cultivating survival rate and increasing reproduction coefficient

Active Publication Date: 2019-05-24
日照新睿招商发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the traditional method of raising seedlings and breeding of dwarf pearls reduces yield, serious virus accumulation, species degeneration, quality and yield drop sharply, and planting benefit declines

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A method for in vitro rapid propagation of water tower flowers, comprising the steps of:

[0028] (1) Obtain explants and sterilize

[0029] Select new young shoots of strong plants, wash the dirt and dust on the surface of the shoots with tap water, soak them in alcohol first, then sterilize them with mercury, and finally rinse them with sterile water to get sterilized explants.

[0030] (2) Primary culture

[0031] The sterilized explants were divided into 1.0cm budding stem segments, then inserted into the primary culture medium and cultivated for 36 days to obtain adventitious buds.

[0032] The primary culture conditions are as follows: temperature is 30°C, humidity is 60%, light time is 10 hours / day, and light intensity is 20001x. On the 1st to 7th day of the primary culture, an ultrasonic field is applied for 4 hours a day, and the power of the ultrasonic field is 15W / cm 2 , the frequency of the ultrasonic field is 28KHz, on the 8th to 15th day of primary cultu...

Embodiment 2

[0045] A method for in vitro rapid propagation of water tower flowers, comprising the steps of:

[0046] (1) Obtain explants and sterilize

[0047] Select new young shoots of strong plants, wash the dirt and dust on the surface of the shoots with tap water, soak them in alcohol first, then sterilize them with mercury, and finally rinse them with sterile water to get sterilized explants.

[0048] (2) Primary culture

[0049] The sterilized explants were divided into 0.6cm budding stem segments, then inserted into the primary culture medium and cultivated for 32 days to obtain adventitious buds.

[0050] The primary culture conditions are as follows: temperature is 26°C, humidity is 55%, light time is 8 hours / day, and light intensity is 18001x. On the 1st to 7th day of primary culture, ultrasonic field is applied for 3 hours every day, and the power of ultrasonic field is 10W / cm 2 , the frequency of the ultrasonic field is 25KHz, on the 8th to 15th day of primary culture, the ...

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PUM

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Abstract

The invention discloses an in-vitro rapid propagation method for billbergia pyramidalis. The method includes the steps: acquiring explants, performing sterilization for the explants, inoculating the sterilized explants into primary culture media, and cultivating the explants to obtain adventitious buds; transferring the adventitious buds into subculture media, and cultivating the adventitious buds to obtain subculture seedlings; transferring the subculture seedlings into rooting culture media, and cultivating the subculture seedlings to obtain billbergia pyramidalis seedlings; hardening the seedlings, and transplanting the hardened seedlings. A preparation method of the primary culture media includes the steps: firstly, adjusting concentration of ferric sulfate and sodium sulfate; secondly, adding aspartic acid and indoleacetic acid based on improved White culture media. A preparation method of the subculture media includes the steps: firstly, adjusting concentration of molybdenum oxide and boracic acid; secondly, adding vitamin K1, indoleacetic acid and naphthylacetic acid based on improved White culture media. A preparation method of the rooting culture media includes the steps: adding ammonium molybdate and gibberellins based on B5 culture media. According to the method, billbergia pyramidalis propagation coefficient is effectively improved, sufficient seedling sources are provided for commercial production of billbergia pyramidalis, and a tissue culture method can effectively shorten seedling period as compared with a sowing propagation method.

Description

technical field [0001] The invention relates to a tissue culture technique, in particular to a method for in vitro rapid propagation of water pagoda flowers. Background technique [0002] Water Tower Flower (Billbergia pyramidalis Lindl.) is a perennial evergreen herbaceous succulent plant of Bromeliaceae, with very short stems. The leaves are broad-lanceolate, sharply pointed, with fine serrations on the edges, hard leathery, bright green, with thick cuticles and absorbing scales on the surface. The spikes are erect, higher than the leaves, the bracts are pink, the corolla is vermilion, the petals are curled, and the edges are purple. The leaves are convoluted and clustered from the rhizome, the base is rosette-shaped, and the center is cylindrical. Bloom more in winter and spring. The leaves are leathery; green and shiny, clustered into rosettes, dignified and beautiful; the bases of the leaves are embracing each other, making the center of the plant into a tube shape, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 不公告发明人
Owner 日照新睿招商发展有限公司
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