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Culture method of concentrated oocystis

A culture method and technology for oocystis, applied in microorganism-based methods, biochemical equipment and methods, unicellular algae, etc., can solve problems such as high cost and damage to microalgae cells, reduce electrostatic resistance, and shorten settling time. , cost saving effect

Active Publication Date: 2017-05-10
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above methods have the disadvantages of damaging microalgae cells and high cost.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Cultivation method of concentrated oocysts in large indoor ponds

[0034] The specification is 1.5m×30m 2 After the indoor pond is cleaned and disinfected, add filtered seawater to a height of 1.2m, and disinfect the water with 1.44kg of 50% strong chlorine essence for 12 hours; Chlorine is all gone, add NaNO 3 2.8kg; K 2 HPO 4 280g; FeC 6 h 5 o 7 (10% solution) 0.72L; Vitamin B 1 7.2g; vitamin B 12 30mg; NaHCO 3 36kg; cytokinin 6-benzylaminopurine 18g, auxin 2.4-dichlorophenoxyacetic acid 40g and oocyst sp. 8 pc / L), adopt the combination of outdoor light and fluorescent lamp as the light source for 24h light cultivation, control the light intensity to 900lux, and pass air and pure CO at the ventilation speed of 0.7vvm 2 The mixed gas is used to suspend microalgae, control the pH value in the water to fluctuate between 8.0 and 8.8, and cultivate at 23 to 27°C for 11 days. Sampling microscope observed the cell concentration of the algae fluid reached 3.0×10 5...

Embodiment 2

[0036] Cultivation method of concentrated oocysts in large indoor ponds

[0037] The specification is 1.5m×44m 2 After the indoor pond is cleaned and disinfected, add filtered seawater to a height of 1.2m, and disinfect the water with 2.1kg of 50% strong chlorine essence for 12 hours; All residual chlorine disappears, add NaNO 3 4.1kg; K 2 HPO 4 410g; FeC 6 h 5 o 7 (10% solution) 1.1L; Vitamin B 1 10.1g; Vitamin B 12 44 mg; NaHCO 3 52kg; 26g of cytokinin 6-benzylaminopurine, 58g of auxin 2.4-dichlorophenoxyacetic acid and 1400L of oocyst sp. 8 pc / L), adopt the combination of outdoor light and fluorescent lamp as the light source for 24h light cultivation, control the light intensity at 950lux, and pass air and pure CO at the ventilation speed of 0.8vvm 2 The mixed gas is used to suspend microalgae, control the pH value in the water to fluctuate between 8.0 and 8.8, and cultivate at 28 to 24°C for 9 days. The cell concentration of the algae liquid reached 3.2×10 unde...

Embodiment 3

[0039] Concentration method for cultivating oocysts in indoor PVC barrels

[0040] For specifications of 2m 3 After the PVC bucket is cleaned and disinfected, add filtered seawater 1.8m 3 , use 72g of 50% strong chlorine essence to disinfect the water for 12h; then use 36g of sodium thiosulfate to neutralize, and keep aerating for 24 hours. After all the residual chlorine in the water disappears, add NaNO 3 144g;K 2 HPO 4 15g; FeC 6 h 5 o 7 (1% solution) 0.36L; Vitamin B 1 360mg; Vitamin B 12 0.9 mg; NaHCO 3 1.8kg; 0.9g of cytokinin 6-benzylaminopurine, 2g of auxin 2.4-dichlorophenoxyacetic acid and 15L of oocyst sp. 8 pc / L), adopt the combination of outdoor light and fluorescent lamp as the light source for 24h light cultivation, control the light intensity at 950lux, and pass air and pure CO at the ventilation speed of 0.8vvm 2 The mixed gas is used to suspend microalgae, control the pH value of the water to fluctuate between 8.2 and 8.6, and cultivate at 25 to 30°...

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Abstract

The invention provides a culture method of concentrated oocystis, which comprises the step of: inoculating algae species into an oocystis culture medium, and carrying out culture under the conditions of a temperature of 22 to 35 DEG C and illumination intensity of 600 to 1,000lux, wherein the oocystis culture medium comprises paddy shallow water 107-13 culture solution and plant hormones; a seawater specific gravity of the oocystis culture medium is 1.008 to 1.016; in the culturing process, a ratio of C to N in algae solution is kept higher than 10:1; and in the culturing process, a pH value of the algae solution is kept between 8.0 and 8.8. According to the method provided by the invention, under the condition of ensuring excellent activity of oocystis cells, the radius and density of an oocystis cell group are increased, so that a settling velocity of the oocystis is promoted by not less than 1.5 times, settling time is shortened, and cost of large-scale culture of the oocystis is reduced.

Description

technical field [0001] The invention relates to the technical field of microalgae cultivation, in particular to a method for cultivating concentrated oocysts. Background technique [0002] Microalgae is a kind of self-supporting plant widely distributed in land and ocean, rich in nutrients and high in photosynthetic utilization. Value-added biological substances can be specifically summarized into the following four categories: proteins, sugars, esters, nucleic acids and various minerals. According to statistics, the typical nutritional composition of microalgae is: the protein content is 40% to 60%, the total sugar content is 10% to 30%, and the total ester content is 10% to 30%. The nutrient composition of different species of microalgae varies greatly. However, the protoplasmic components of most microalgae are denser than water, and the density of sugars is about 1500kg / m 3 , the protein is about 1300kg / m 3 , nucleic acid is about 1700kg / m 3 , only the density of li...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 黄翔鹄李长玲韩谦
Owner GUANGDONG OCEAN UNIVERSITY