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Industrial storage and reutilization method of anaerobic ammonia oxidizing bacteria

A kind of anammox bacteria and anaerobic ammonium oxidation technology, applied in the direction of anaerobic digestion treatment, bacteria, preserved microorganisms, etc., to achieve the effect of simple control conditions, few control factors, and reliable recovery of activity

Active Publication Date: 2017-05-10
BEIJING MUNICIPAL RES INST OF ENVIRONMENT PROTECTION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although these techniques can retain bacterial activity to a certain extent, whether they can be directly applied to the preservation process of anammox bacteria needs to be supported by the accumulation of considerable experimental data.

Method used

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  • Industrial storage and reutilization method of anaerobic ammonia oxidizing bacteria
  • Industrial storage and reutilization method of anaerobic ammonia oxidizing bacteria

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Effect test

Embodiment 1

[0041] The anammox strain storage container in the present embodiment is as figure 1 shown. The strain storage container 1 is a cylindrical stainless steel tank with an effective volume of 1000L. The top of the strain storage container 1 is provided with a flange feed port 2, and the bottom is provided with a flange discharge port 4. The tank body of the strain storage container 1 is provided with a plate frame sight glass 3, which can observe the liquid level and the mud level in the tank. The inner bottom of the strain storage container 1 is provided with a ring-shaped microporous aeration pipe 5, which is connected to a high-purity argon gas source 9 through an air inlet pipe 6, and an air inlet valve 7 and a gas heating device 8 are arranged on the air inlet pipe 6 . The middle part of the strain storage container 1 is provided with a drain pipe 12 on which a drain ball valve 13 is arranged. A vacuum pumping system is provided on the drain pipe 12, including a negative...

Embodiment 2

[0057] The anammox strain storage container in the present embodiment is as figure 1 shown. The strain storage container 1 is a cylindrical pressure-resistant glass fiber reinforced plastic tank with an effective volume of 1000L. The top of the strain storage container 1 is provided with a flange feed port 2, and the bottom is provided with a flange discharge port 4. The tank body of the strain storage container 1 is provided with a plate frame sight glass 3, which can observe the liquid level and the mud level in the tank. The inner bottom of the strain storage container 1 is provided with a ring-shaped microporous aeration pipe 5, which is connected to a high-purity argon gas source 9 through an air inlet pipe 6, and an air inlet valve 7 and a gas heating device 8 are arranged on the air inlet pipe 6 . The middle part of the strain storage container 1 is provided with a drain pipe 12 on which a drain ball valve 13 is arranged. A vacuum pumping system is provided on the d...

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Abstract

The invention provides an industrial storage and reutilization method of anaerobic ammonia oxidizing bacteria and belongs to the field of biological treatment of wastewater. The method comprises the following three steps of selecting the anaerobic ammonia oxidizing bacteria, storing and recovering activity: step 1, taking out active sludge from different parts of an anaerobic ammonia oxidizing reactor which is operated normally, and uniformly mixing; introducing high-purity argon gas; selecting the suitable anaerobic ammonia oxidizing bacteria; step 2, precipitating and washing the selected bacteria and then adding a storage matrix; introducing the high-purity argon gas, and selecting environment conditions including temperature, a matrix concentration and the like in a storage process according to different storage periods; step 3, carrying out activity recovery operation of controllable decompression and temperature rising, and step load on the stored anaerobic ammonia oxidizing bacteria, so as to rapidly obtain high-activity anaerobic ammonia oxidizing bacteria capable of being industrially reutilized. According to the industrial storage and reutilization method, high-activity storage of the anaerobic ammonia oxidizing bacteria is realized and operation factors are control conditions which are easily industrially realized; the industrial storage and reutilization method is simple and convenient to operate and low in cost, and has a very high application value.

Description

technical field [0001] The invention belongs to the field of wastewater biological treatment and relates to a method for storing and reusing anaerobic nitrogen oxidizing bacteria. Background technique [0002] Since its discovery in 1995, the anammox process has been recognized as a new type of high-efficiency and low-cost biological denitrification technology. Since anaerobic nitrogen oxidizing bacteria can convert inorganic CO 3 - As the only carbon source for growth and metabolism, no organic carbon source is needed; no oxygen is needed; its ammonia oxidation rate is 25 times that of aerobic nitrifying bacteria; the total nitrogen removal load that can be achieved is dozens of times that of traditional nitrification and denitrification processes; pollution The mud yield is extremely low. The above excellent process characteristics make the anaerobic ammonium oxidation technology highlight its economy in the field of biological denitrification, and it has natural adapta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/04C02F3/28
CPCC02F3/28C12N1/04C12N1/20
Inventor 曹建平杜兵刘寅何然王珊
Owner BEIJING MUNICIPAL RES INST OF ENVIRONMENT PROTECTION
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