Penicillin fermentation production process multi-model monitoring system based on near infrared spectroscopy technology

Abstract

The invention discloses a penicillin fermentation production process multi-model monitoring system based on a near infrared spectroscopy technology, and belongs to the crossing field of near infrared spectroscopy and penicillin process monitoring. A multi-model analysis method based on the near infrared spectroscopy technology realizes the real-time on-line detection and monitoring on biochemical indexes of fermentation liquid biomass, sugar content, nitrogen content, penicillin concentration and the like in the penicillin fermentation process; the precise value and change trends can be obtained. According to the technical scheme provided by the invention, the penicillin fermentation monitoring system comprises a hardware device consisting of a near infrared spectrograph, a PLC (programmable logic controller) and an industrial personal computer, and a software part consisting of a distinguishing model, a regression model and the like formed by a chemical metrology method. Each part works in a cooperated way; the structure is clear; the system is complete; the automation degree of the penicillin fermentation process can be well improved.

Description

[0001] 1. Technical field [0002] The invention relates to the technical field of on-line detection of liquid fermentation near-infrared spectra, in particular to a system for collecting fermentation liquid spectra online with a near-infrared spectrometer and monitoring the process with multiple measurement sub-models for the penicillin fermentation process. [0003] 2. Background technology [0004] Penicillin is a β-lactam antibiotic, which is a metabolite during the growth of Penicillium. Penicillin can inhibit transpeptidase, prevent the mucopeptide cross-linking in the synthesis of bacterial cell walls, and hinder the synthesis of bacterial cell walls, thereby inhibiting bacterial growth. grow. Penicillin has been used clinically since the 1840s, saving hundreds of millions of lives. In 1997, the total production of penicillin worldwide reached 38,000 tons. Penicillin can not only be used for oral administration and injection, but also can be used as a raw material for ...

AI Technical Summary

Problems solved by technology

However, it is difficult to carry out real-time online detection of the important parameters of the fermentation process, such as the sugar content, nitrogen content, cell concentration, and penicillin product concentration of the fermentation broth.

Although major companies have developed optical instruments such as refractometers and turbidimeters, due to the complex composition of the fermentation broth in the fermentation environment, it is difficult for the above instruments to perform effective detection, and the accuracy cannot be guaranteed

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