Detecting and analyzing method for anthocyanins substances in blueberries
An analysis method, the technology of anthocyanins, applied in the field of detection and analysis, can solve the problems such as inconspicuous advantages, and achieve the effects of good repeatability and stability, low detection limit, fast and accurate qualitative and quantitative detection
Active Publication Date: 2017-05-10
SHANDONG INST OF POMOLOGY
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Dang Ya et al. (Chinese Condiment, Vol. 40, No. 5: 76-81) disclosed a study on the extraction and component analysis of blueberry anthocyanins, using ultra-high performance liquid chromatography-triple quadrupole tandem mass spectrometry Anthocyanins in blueberries were analyzed, but only four components were detected, and the advantages were not obvious
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[0036] A method for detecting and analyzing anthocyanins in blueberries, comprising the following steps:
[0037] (1) Take 0.5g of the peel of ripe blueberry fruit with a thickness of 1-2mm, grind it with liquid nitrogen, put it in a centrifuge tube, add methanol-0.15% formic acid water-cyclohexanol 15 mL( The volume ratio of methanol-0.15% formic acid to water-cyclohexylhexanol is 2:8:5), after ultrasonic vibration for 10 minutes, first store it at -15°C for 10 minutes, take it out and return it to room temperature, and then place it at 4°C Under leaching for 24 hours, centrifuged, and the supernatant was collected;
[0038] (2) Purify and elute the supernatant through the fully activated HPD-450A macroporous resin loaded into a glass chromatography column, collect the eluate, evaporate it to an extract at 30°C, and wash it with methanol-0.1% formic acid Redissolve in aqueous solution (the volume ratio of methanol-0.1% formic acid aqueous solution is 2:8), dilute to 2mL, an...
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The invention belongs to the technical field of detection analysis, and particularly relates to a detecting and analyzing method for anthocyanins substances in blueberries. The method comprises the following specific steps: taking peels of ripe blueberries, adding liquid nitrogen and grinding, then placing in a centrifugal tube, adding methanol-0.15% methanoic acid water-inositol, carrying out ultrasonic oscillating, storing at the temperature of minus 15 DEG C, taking out and recovering to the room temperature, then placing in an environment at the temperature of 4 DEG C and carrying out leaching, centrifuging and collecting supernate; and loading the supernate in completely activated macroporous resin of a glass chromatography column, purifying and eluting, collecting eluent, evaporating the eluent to obtain extract, redissolving with a methanol-0.1% methanoic acid water and carrying out constant volume, and filtering; and analyzing and detecting filtrate by using an ultra-efficient liquid chromatography-mass spectrometry. Conditions such as a moving phase, an elution program and a chromatographic column are screened and optimized, and the selected method is good in repeatability and stability; and the anthocyanins substances in the blueberries can be rapidly and accurately detected qualitatively and quantitatively, and the detection limit is low.
Description
technical field [0001] The invention belongs to the technical field of detection and analysis, and in particular relates to a method for detection and analysis of anthocyanins in blueberries. Background technique [0002] Blueberry (Blueberry) belongs to Ericaceae (Ericaceae) Vaccinium (Vaccinium) plant, is a new fruit tree, rich in anthocyanins, ursolic acid, flavonoids and other antioxidant substances, can improve vision, enhance memory, soften blood vessels , anti-cancer and other functions, much favored by consumers, has broad prospects for development. The antioxidant activity of blueberry fruit is mainly determined by its rich anthocyanins. Therefore, the content of anthocyanins in blueberries is one of the important factors to determine its quality. [0003] Anthocyanin is a water-soluble natural pigment widely present in plants, which belongs to flavonoids, and mainly appears blue, purple and red in plants. At present, the research on anthocyanins at home and abro...
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IPC IPC(8): G01N30/02G01N30/06G01N30/12G01N30/72G01N30/34
CPCG01N30/02G01N30/06G01N30/12G01N30/34G01N30/72G01N2030/065
Inventor 魏海蓉刘庆忠易锡斌陈新谭钺宗晓娟徐丽朱东姿
Owner SHANDONG INST OF POMOLOGY