A method for improving the quality of re-cured tobacco leaves by fermentation of Chrysanthemum tea leaves
A technology for fermenting re-cured tobacco leaves and tea leaves, which is applied in the field of tobacco processing, and can solve the problems of poor taste and quality of re-cured tobacco leaves, and achieve the effects of improving quality and usability, improving aroma quality, and increasing aroma
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Embodiment 1
[0025] Example 1 strain screening
[0026] The coronoid saccharomyces strain of the present invention is isolated from Liubao tea produced in Wuzhou, Guangxi, and specifically comprises the following steps:
[0027] (1) Use enrichment medium to isolate microbial strains from Liubao tea, grind Liubao tea and add enrichment medium to cultivate and screen. The enrichment medium is: 1g K 2 HPO 4 ·3H 2 O, 0.5g MgSO 4 ·7H 2 O, 3g NH 4 NO 3 , 32gNaCl, 20g sucrose, 1000mL water, pH 6.0;
[0028] (2) The obtained microbial bacterium liquid is screened on the flat plate separation medium by the flat coating method, and after constant temperature cultivation, the bacterial colonies with large morphological differences are selected to be streaked and purified respectively until a single colony occurs, and the bacterial strain is obtained, kept, and used for future use; The plate separation medium is: 1g K 2 HPO 4 ·3H 2 O, 0.5g MgSO 4 ·7H 2 O, 3g NH 4 NO 3 , 32g NaCl, 20g suc...
Embodiment 2
[0032] Inoculate the bacterial strains screened in Example 1 of refrigerated preservation on the PDA slant medium with an inoculation needle, and place it at 30° C. to cultivate for 48 hours. ℃ inverted culture for 7 days.
[0033] The conidia culture medium is: 1g K 2 HPO 4 ·3H 2 O, 0.5g MgSO 4·7H 2 O, 3g NH 4 NO 3 , 32gNaCl, 20g sucrose, 15g agar powder, water 1000mL.
[0034] The conidia on the mycelium were washed off with sterile water, and the concentration of the spores was 10 6 -10 7 / mL of spore suspension.
[0035] Take 1 mL of the obtained spore suspension and dilute it with sterile water to obtain 5 g of the spore suspension, and mix well.
[0036] According to the proportion of 1wt% of the tea weight, the diluted spore suspension was evenly sprayed on 500 g of Liubao tea leaves (Guangxi), and then the tea leaves were placed at 28° C. for conventional composting and cultured for 48 hours to obtain Jinhua fungus tea leaves.
Embodiment 3
[0038] Add the chrysanthemum tea leaves prepared in Example 2 to 10Kg 2014 Guangxi Hezhou Zhongjusan C3F re-baked tobacco according to 0.2% of the weight of the tobacco leaves and mix evenly, and then seal the tobacco leaves and place them in a tobacco leaf storage warehouse for fermentation and alcoholization. The temperature of the tobacco leaf warehouse is room temperature, and the relative air humidity is ≤75%. Tobacco leaves were fermented and alcoholized for 60 days. The alcoholized tobacco leaves were evaluated and smoked by a panel of 7 experts. It was found that the alcoholic tobacco leaves had significantly reduced miscellaneous gases, decreased irritation, soft and delicate smoke, increased sweetness, increased tobacco aroma, and There is a tea aroma, and the quality and usability of the tobacco leaves are significantly improved.
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