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Bacillus amyloliquefaciens and application thereof to degradation of carbamate pesticides

A technology of carbamates and amyloliquefaciens, which is applied to Bacillus amyloliquefaciens and its application in the degradation of carbamate pesticides, which can solve the problems of slow degradation rate and degradation rate of only 60%, and achieve rapid degradation , good application prospects, and the effect of protecting the ecological environment

Active Publication Date: 2017-05-17
CHENGDU MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the degradation rate of existing bacteria to carbamate pesticides is slow as, the publication number is that the bacteria disclosed in CN1723276A acted on carbamate compounds for 10 days, and the degradation rate was only 60%.

Method used

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  • Bacillus amyloliquefaciens and application thereof to degradation of carbamate pesticides
  • Bacillus amyloliquefaciens and application thereof to degradation of carbamate pesticides
  • Bacillus amyloliquefaciens and application thereof to degradation of carbamate pesticides

Examples

Experimental program
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Effect test

Embodiment 1

[0028] Embodiment 1 Isolation and identification of Bacillus amyloliquefaciens of the present invention

[0029] 1. Separation

[0030] Take 4 parts of vinegar sample, mix well respectively, take 5g each, place in a 250mL Erlenmeyer flask filled with 30mL LB medium, heat at 80°C for 10min, cool to room temperature and then add carbaryl to make the mass concentration 50 mg / L, shake culture at 30°C, 180r / min for 48h. After the end of the culture, the enrichment culture was carried out successively 3 times according to the inoculum size of 5%, each culture was 48h, and the concentration of carbaryl in the culture medium was gradually increased to make it reach 100mg / L, 200mg / L, 400mg / L respectively, and the obtained A pesticide-degrading bacterium, numbered JX-6.

[0031] 2. Identification

[0032] (1) Morphological, physiological and biochemical characteristics

[0033] Morphology: Pick the bacterial strain JX-6 and cultivate it on LB solid medium plate, record the morpholog...

Embodiment 2

[0043] Embodiment 2 Adopt Bacillus amyloliquefaciens of the present invention to degrade carbaryl in soil

[0044] Take Bacillus amyloliquefaciens JX-6 of the present invention, the preservation number is CGMCC No. 13143, referred to as strain JX-6. Strain activation: pick strain JX-6 into LB liquid medium, 30°C, 180r / min shaking culture for 24h; The LB slant was cultured at 30°C for 2 days for later use. Wash the bacterial cells on the lower slant with sterile saline and adjust the cell concentration to 10 6 cfu / mL, made into seed solution.

[0045] Add the seed liquid to the soil to be treated, mix well, and that's it.

Embodiment 3

[0046] Example 3 Using Bacillus amyloliquefaciens of the present invention to degrade carbaryl in water

[0047] Take Bacillus amyloliquefaciens JX-6 of the present invention, the preservation number is CGMCC No. 13143, referred to as strain JX-6. Strain activation: pick strain JX-6 into LB liquid medium, 30°C, 180r / min shaking culture for 24h; The LB slant was cultured at 30°C for 2 days for later use. Wash the bacterial cells on the lower slant with sterile saline and adjust the cell concentration to 10 8 cfu / mL, made into seed solution.

[0048] Add the seed liquid into the sample water to be treated, mix well, and that's it.

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PUM

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Abstract

The invention discloses bacillus amyloliquefaciens which is bacillus amyloliquefaciens JX-6 with a preservation number of CGMCC No. 13143 preserved in the China General Microbiological Culture Collection Center. The bacillus amyloliquefaciens JX-6 with the preservation number of CGMCC No. 13143, disclosed by the invention, can effectively degrade the carbamate pesticides and can be used for bioremediation or biological purification of soil and water bodies polluted by substances to protect ecological environment, thereby being excellent in application prospect.

Description

technical field [0001] The invention relates to a strain of bacillus amyloliquefaciens and its application in degrading carbamate pesticides. Background technique [0002] Carbamate (general formula RO(CO)NRR) pesticides are synthetic pesticides developed after organophosphates, and are widely used in agricultural production such as insecticide, acaricide, and weed control. Carbamate pesticides generally have no special odor, are stable in acidic environments, decompose in case of alkali, and have high solubility in water. Most species are less toxic than organic phosphates and are not highly toxic compounds, but they are carcinogenic and can invade the body through the respiratory and digestive tracts, and can also be slowly absorbed through the skin and mucous membranes. Carbamate pesticides have mutagenic, teratogenic and carcinogenic effects. For example, treating mice and rats with carbaryl in various ways can cause cancer and has teratogenic effects on guinea pigs, d...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02A62D101/04C12R1/07
CPCA62D3/02A62D2101/04C12N1/205C12R2001/07
Inventor 李建龙代敏龙娜娜陈春琳
Owner CHENGDU MEDICAL COLLEGE
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