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Preparation method of infection response type guide tissue regeneration membrane

A technology that guides tissue regeneration and response, applied in medical science, prostheses, coatings, etc., can solve problems that easily cause gastrointestinal side effects, low drug utilization, and affect the acquisition of new tissues

Active Publication Date: 2017-05-24
BEIJING UNIV OF CHEM TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although GTR technology can achieve long-term and stable curative effect, the infection caused by anaerobic bacteria and aerobic bacteria introduced into the injury site during and after surgery and during the second removal process will affect the acquisition of new tissue
At present, systemic medication is mainly used for postoperative anti-infection, but the drug utilization rate is low, and it is easy to cause gastrointestinal side effects

Method used

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  • Preparation method of infection response type guide tissue regeneration membrane
  • Preparation method of infection response type guide tissue regeneration membrane
  • Preparation method of infection response type guide tissue regeneration membrane

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Cut the PCL nanofibers prepared by electrospinning into discs with a diameter of 2 cm, and soak them in 1.0 g / L dopamine solution (with dopamine hydrochloride: tris(hydroxymethylaminomethane)=5:3 (molar ratio) ), the pH value of the solution is 8.5), after 12 hours of reaction under stirring, the spinning membrane is taken out, and the unreacted dopamine solution is removed by dipping.

[0029] (2) The polycaprolactone nanofibers prepared by electrospinning obtained in step (1) were soaked in a 5.0 g / L silane coupling agent KH550 solution, reacted at 40° C. for 24 h respectively, and the spinning The silk membrane was taken out, and the unreacted KH550 was removed by dipping.

[0030] (3) introduce ester bond on drug molecule: metronidazole reacts with acryloyl chloride, thereby introducing ester bond into drug metronidazole; reaction process is as follows: 1. take by weighing drug metronidazole: acryloyl chloride: triethylamine=1 : 1:1 (molar ratio), chloroform. ...

Embodiment 2

[0033] The polycaprolactone nanofibers in Example 1 were replaced with cellulose films, and other experimental conditions were the same as those in Example 1. By comparing the SEM images of the fibrous membrane a before dopamine coating and the fibrous membrane b after dopamine coating, it can be found that the surface of b is coated with a layer, and with the prolongation of immersion in dopamine, the coating amount increases, indicating that dopamine is successful Grafted to the surface of the fiber membrane; the fiber membrane after KH550 graft modification is marked as c, and the infrared spectra of b and c are compared, at 3000-3500cm -1 The absorption peak of hydroxyl (-OH) at the wave number disappears, indicating that KH550 is grafted to the fiber membrane through the reaction between the ethoxy group on KH550 and the hydroxyl (-OH) on the polydopamine shell, thus making the hydroxyl (-OH) Absorption peak disappeared; 1000-1100cm -1 The wavenumber is the absorption pe...

Embodiment 3

[0035] The γ-aminopropyltriethoxysilane (KH550) in Example 1 was replaced with 3-aminopropyltrimethoxysilane (A-1110), and other experimental conditions were the same as those in Example 1. Comparing the infrared spectra of the polycaprolactone nanofiber membrane obtained by dopamine coating and the polycaprolactone nanofiber membrane further obtained by A-1110 graft modification, at 3000-3500cm -1 The absorption peak of hydroxyl (-OH) disappears at the wavenumber, indicating that 3-aminopropyltrimethoxysilane (A-1110) is connected to the hydroxyl (-OH) on the polydopamine shell through the reaction between the methoxy group on A-1110 and the polydopamine shell. branched to polycaprolactone nanofibers, thus making the absorption peak of hydroxyl (-OH) disappear; 1000-1100cm -1 The wavenumber is the absorption peak of Si-O-Si bond, indicating that A-1110 has been grafted onto the polycaprolactone nanofibers.

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Abstract

The invention discloses a preparation method of an infection response type guide tissue regeneration membrane. The guide tissue regeneration membrane material is a degradable polyester material and a natural degradable macromolecular material, and an antibacterial medicine with hydroxyl is chemically bound to the surface of the guide tissue regeneration membrane by using an ester bond. The method particularly comprises the following steps: introducing a large amount of hydroxyl into the surface of the membrane through dopamine coating; conducting reaction on a silicon oxygen bond on a silane coupling agent and the hydroxyl on the surface of the membrane so as to introduce amino into the surface of the membrane; conducting reaction on the antibacterial medicine with the hydroxyl and acryloyl chloride, and introducing the ester bond and carbon-carbon double bond into the medicine; performing Michael addition reaction on the carbon-carbon double bond and the amino on the surface of the membrane so as to graft the medicine to the surface of the fibrous membrane through the ester bond. The infection response type guide tissue regeneration membrane provided by the invention has excellent biocompatibility and capability of controllably releasing the antibacterial medicine according to the infection degree so as to fulfill the aim of effectively inhibiting infection.

Description

technical field [0001] The invention belongs to the field of material surface modification, and in particular relates to a preparation method of an infection-responsive guided tissue regeneration membrane that releases antibacterial drugs. Background technique [0002] Guide Tissue Regeneration (GTR) technology is a new technology developed in the late 1980s and early 1990s. The principle is to use the physical barrier function of the membrane to isolate the damaged part from the surrounding tissue, creating a relatively closed tissue environment, so that osteoblasts can migrate and grow preferentially. The application of GTR provides a new and effective way for the treatment of periodontal disease, the restoration of insufficient bone mass in the dental implant area and other bone defects, and the healing of fractures. [0003] Although GTR technology can achieve long-term and stable curative effect, the infection caused by anaerobic bacteria and aerobic bacteria introduce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/58A61L27/54A61L27/18
CPCA61L27/18A61L27/54A61L27/58A61L2300/216A61L2300/404A61L2300/606C08L67/04
Inventor 张立群叶静静石锐田伟
Owner BEIJING UNIV OF CHEM TECH