Preparation method of an infection-responsive guided tissue regeneration membrane
A technology that guides tissue regeneration and response, applied in medical science, prostheses, coatings, etc., can solve problems that easily cause gastrointestinal side effects, low drug utilization, and affect the acquisition of new tissues
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Embodiment 1
[0028] (1) The PCL nanofiber prepared by electrospinning is cut into discs with a diameter of 2 cm, soaked in a 1.0 g / L dopamine solution (with dopamine hydrochloride:trishydroxymethylaminomethane=5:3 (molar ratio ) prepared dopamine solution, the pH value of the solution is 8.5), after reacting for 12 hours under stirring, the spinning membrane was taken out, and the unreacted dopamine solution was removed by rinsing.
[0029] (2) The polycaprolactone nanofibers prepared by electrospinning obtained in step (1) were immersed in the silane coupling agent KH550 solution with a concentration of 5.0 g / L, and reacted for 24 hours at 40 ° C respectively, and the spun Take out the silk film and wash to remove unreacted KH550.
[0030] (3) Introducing an ester bond on the drug molecule: Metronidazole reacts with acryloyl chloride, thereby introducing the ester bond into the drug metronidazole; the reaction process is as follows: 1. Weigh the drug metronidazole: acryloyl chloride: trie...
Embodiment 2
[0033] The polycaprolactone nanofibers in Example 1 were replaced with cellulose films, and other experimental conditions were the same as in Example 1. By comparing the SEM images of fibrous membrane a before coating with dopamine and fiber membrane b after coating with dopamine, it can be found that the surface of b is coated with a layer, and as the soaking time in dopamine prolongs, the coating amount increases, indicating that dopamine successfully Grafted to the surface of the fiber membrane; the fiber membrane modified by KH550 grafting is recorded as c, compare the infrared spectra of b and c, at 3000-3500cm -1 The absorption peak of the hydroxyl group (-OH) at the wave number disappeared, indicating that KH550 was grafted onto the fiber membrane through the reaction of the ethoxy group on the KH550 and the hydroxyl group (-OH) on the polydopamine shell, thus making the hydroxyl group (-OH) The absorption peak disappears; 1000-1100cm -1 The wave number is the absorpti...
Embodiment 3
[0035] The γ-aminopropyltriethoxysilane (KH550) in Example 1 was replaced with 3-aminopropyltrimethoxysilane (A-1110), and other experimental conditions were the same as in Example 1. Compare the infrared spectrum of the polycaprolactone nanofiber membrane obtained by coating with dopamine and the polycaprolactone nanofiber membrane obtained by A-1110 graft modification, at 3000-3500cm -1 The absorption peak of the hydroxyl group (-OH) at the wave number disappears, indicating that 3-aminopropyltrimethoxysilane (A-1110) reacts with the hydroxyl group (-OH) on the polydopamine shell through the reaction of the methoxy group on the A-1110 branched onto polycaprolactone nanofibers, thus making the absorption peak of the hydroxyl group (-OH) disappear; 1000-1100cm -1 The wave number is the absorption peak of Si-O-Si bond, indicating that A-1110 has been grafted onto polycaprolactone nanofibers.
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