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Long-term preservation method of dendrobium officinale capsule/seed

A technology of Dendrobium officinale and a preservation method, which is applied to the preservation of plants, treatment of seeds and rhizomes, and botanical equipment and methods, can solve the problems of reduced seed germination rate, difficulty in collecting and sterilizing seeds, etc., and achieves long preservation time and germination. High efficiency and simple operation

Active Publication Date: 2021-07-23
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the fruit is harvested when it is ripe and cracked, it will not only be difficult to collect and disinfect the seeds, but also the germination rate of the seeds will decrease

Method used

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  • Long-term preservation method of dendrobium officinale capsule/seed
  • Long-term preservation method of dendrobium officinale capsule/seed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Embodiment 1, the preservation method of the Dendrobium officinale capsule, the Dendrobium officinale capsule is gathered in late October, selects uncracked fruit (that is, complete fruit), and carries out the following steps successively:

[0064] 1), fruit disinfection:

[0065] Dip 70% (volume %) alcohol cotton balls to wipe the fruit surface, then transfer to the ultra-clean workbench, soak the fruit for 30 seconds with 70% alcohol, rinse once with sterile water, and then rinse with 0.1% (mass %) ) mercury chloride (0.1% mercury chloride plus 3 to 5 drops of Tween 20 per 100 mL) was sterilized for 10 minutes, rinsed with sterile water, and placed on sterile filter paper to absorb the moisture.

[0066] Tween 20, that is, TWEEN-20 (Shanghai Sangon Bioengineering Co., Ltd., subpackaged by AMRESCO).

[0067] 2), the dehydration treatment of fruit:

[0068] Put the aseptic capsules of step 1) into sterilized square filter paper (30 cm * 30 cm) per 30 and wrap them tig...

Embodiment 2

[0073] Embodiment 2, a kind of preservation method of Dendrobium candidum seed, the Dendrobium candidum capsule is gathered in late October, picks out the dehiscence fruit from the Dendrobium candidum fruit of harvest; Carry out the following steps successively to the dehiscence fruit:

[0074] 1), disinfection of fruit:

[0075] The dehiscing fruit is wiped on the surface with 70% (volume %) alcohol;

[0076] 2), the dehydration treatment of fruit:

[0077] Pour 100 grams of silica gel into a large petri dish with a diameter of 15 cm, spread a layer of 12 cm square filter paper on top of the silica gel, cut the fruit after wiping and disinfecting the above surface, and shake the seeds onto the filter paper of the petri dish until there is a thin layer of seeds all over the filter paper. Then use plastic wrap to seal the contact between the upper and lower covers of the culture dish, place it at room temperature, replace the silica gel every 2 days, and end the dehydration p...

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Abstract

The invention discloses a method for preserving dendrobium candidum capsules. Harvested dendrobium candidum fruits are divided into intact fruits and cracked fruits, and the following steps are sequentially performed on the intact fruits: fruit disinfection; fruit dehydration treatment; dehydrated capsules are subjected to water content detection ;Put the fruit wrapped in filter paper into a sealed bag filled with silica gel, and put at least one sealed bag into a sealed container filled with silica gel, and store the entire sealed container at -80°C to -20°C. The invention also discloses a corresponding method for preserving the cracked fruit.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for long-term preservation of dendrobium officinale capsules and seeds of the rare orchid family medicinal material. Background technique [0002] Dendrobium officinale is a perennial epiphytic orchid of the genus Dendrobium in the family Orchidaceae. "Chinese Pharmacopoeia" (2010 edition) regards Dendrobium officinale Kimura et Migo as a single medicinal species, and clarifies that its base plant is Dendrobium officinale Kimura et Migo, and its dried stems or fresh stems are used for medicine (National Pharmacopoeia Commission, 2010). Dendrobium officinale contains polysaccharides, stilbenes, alkaloids, amino acids, and trace elements, and has various biological activities such as enhancing immunity, anti-oxidation, anti-tumor, anti-fatigue, lowering blood sugar, and nourishing yin and promoting body fluid (Li Ling et al., 2011 ). [0003] At present, the expl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01C1/00A01N3/00
CPCA01C1/00A01N3/00
Inventor 姜维梅傅承新
Owner ZHEJIANG UNIV
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