Application of codonopsis pilosula pectic polysaccharides (CPP1c) and drug and health product thereof

A technology of pectin polysaccharides and health products, applied in the field of biomedical engineering, can solve the problems of low medicinal value and health care value, and achieve the effects of promoting the secretion of immune-related molecules, promoting the expression of immune-related genes, and promoting the expression of immune-related proteins

Active Publication Date: 2017-05-31
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, little is known about the medicinal and health value of Codonopsis polysaccharides, especially Codonopsis pectin polysaccharide CCP1c

Method used

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  • Application of codonopsis pilosula pectic polysaccharides (CPP1c) and drug and health product thereof
  • Application of codonopsis pilosula pectic polysaccharides (CPP1c) and drug and health product thereof
  • Application of codonopsis pilosula pectic polysaccharides (CPP1c) and drug and health product thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] This example provides the preparation and detection methods of Codonopsis pectin polysaccharide CPP1c.

[0069] The extraction method of Codonopsis pectin polysaccharide CPP1c comprises the following steps:

[0070] 1.1 Preparation and extraction of Codonopsis polysaccharide CPP1: Codonopsis pilosula is crushed, degreased with 95% ethanol, extracted in hot water at a temperature above 90°C, concentrated, and precipitated with ethanol with a final concentration of 10-40% , filtered, precipitated as crude Codonopsis polysaccharide CPP1, and protein and pigment were removed.

[0071] 1.2 Weigh 100 mg of the CPP1 sample after deproteinization and depigmentation, dissolve it in 100 mL of water filtered through a 0.45 μm filter membrane, centrifuge (3000 rpm, 3 min), and take the supernatant. After the DE-52 cellulose column was equilibrated with water filtered through a 0.45 μm filter membrane (about 12 h), the above prepared solution was loaded with a flow rate of 25 mL / h....

Embodiment 2

[0092] This example provides the application of Codonopsis pectin polysaccharide CPP1c in the preparation of drugs for promoting lymphocyte proliferation.

[0093] In vitro experiments

[0094] Part I, Preparation of Lymphocytes

[0095] Separate the spleen of SAMP8 mice or normal mice obtained under sterile conditions with lymphocyte separation medium, transfer it to a centrifuge tube, and carefully add 1640 medium along the tube wall to make it form with the separation medium interface. Centrifuge at 3000rpm for 0.5h. Carefully suck out the misty splenocyte layer with a pipette, wash with culture medium, and adjust the cell concentration to the desired concentration.

[0096] Part II, Lymphocyte Proliferation Experiment

[0097] 1.1 Experimental grouping, normal group: ordinary mouse spleen lymphocytes + blank medium; model group: SAMP8 mouse spleen lymphocytes + blank medium; Codonopsis pectin polysaccharide CPP1c administration group: SAMP8 mouse spleen lymphocytes + C...

Embodiment 3

[0105] This example provides the application of Codonopsis pectin polysaccharide CPP1c in the preparation of drugs for improving lymphocyte activity.

[0106] In vitro experiments

[0107] Refer to the mouse lymphocytes and grouping prepared in Example 2.

[0108] Carry out the experiment of in vitro lymphocyte activity; The method is as follows:

[0109] 1.1 Plant the splenic lymphocyte suspension of ordinary mice or SAMP8 mice in 24-well plates, 500 μL per well (about 1×10 7 indivual).

[0110] 1.2 According to the normal group: ordinary mouse spleen lymphocytes + blank medium; model group: SAMP8 mouse spleen lymphocytes + blank medium; Codonopsis pectin polysaccharide CPP1c administration group: SAMP8 mouse spleen lymphocytes + CPP1c (CPP1c The final concentration is 50 μg / mL, 100 μg / mL, 200 μg / mL) into groups; add blank culture medium to the normal group and model group respectively, and add gradient concentration of Codonopsis pectin polysaccharide CPP1c to the Codonop...

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Abstract

The invention provides application of codonopsis pilosula pectic polysaccharides (CPP1c) and a drug and a health care product thereof, and belongs to the field of biomedical engineering. The codonopsis pilosula pectic polysaccharides (CPP1c) can promote the proliferation of lymphocyte, improve the activity of lymphocyte, promote the expression of immune related molecules and related gene and promote the homing of lymphocyte. The codonopsis pilosula pectic polysaccharides (CPP1c) have high medicine value and are high in health care function; the drug and the health care product prepared by the codonopsis pilosula pectic polysaccharides (CPP1c) have food effect and are worthy of popularization and application.

Description

technical field [0001] The invention relates to the field of biomedical engineering, and in particular to the application of Codonopsis pectin polysaccharide CPP1c and its medicine and health care product. Background technique [0002] Codonopsis pilosula (Franch.) Nann.f), Codonopsis pilosula Nann.f var.modesta (Nann.f) L.T.Shen) and Sichuan Codonopsis pilosula (Codonopsistang shen O.liv) dry roots. Sweet and flat in nature, it has the effects of invigorating the middle and Qi, invigorating the spleen and lungs, promoting body fluid and harmonizing the stomach. Modern pharmacological studies have confirmed that Codonopsis pilosula has the functions of enhancing the body's immunity, improving gastrointestinal function, regulating blood sugar, scavenging free radicals, anti-inflammation, and anti-oxidation. Phytochemical studies have shown that Codonopsis pilosula mainly contains various compounds such as alkaloids, phenylpropanoids, triterpenes, polyacetylenes, sterols, vo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/702A61P37/04
CPCA61K31/702A61K36/344
Inventor 胡芳弟苟于强白瑞斌孙伯禄
Owner LANZHOU UNIVERSITY
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