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Method for efficiently extracting chlorogenic acid from dandelion

A technology of dandelion and chlorogenic acid, applied in the separation/purification of carboxylic acid esters, organic chemistry, etc., can solve the problems of low economic value of dandelion, low utilization rate of dandelion, and single extraction method, so as to improve the sweetness and ease of operation , The effect of low extraction cost

Inactive Publication Date: 2017-05-31
马健
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many researches on the extraction of dandelion in China, but the extraction methods are very single, mainly water extraction and alcohol extraction, the extraction rate is low, and the utilization rate of dandelion is low, so the economic value of dandelion is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The method for efficiently extracting chlorogenic acid in dandelion comprises the following steps:

[0018] (1) Put the dandelion dry powder in a clean container, add deionized water solvent to dissolve the dandelion dry powder according to the material-to-liquid ratio of 1:10 / ml, and set aside;

[0019] (2) Add an enzyme solution to the dandelion dry powder solution, the ratio of the added enzyme solution to the dandelion dry powder is 1:2g / ml, and place it at a temperature of 40°C for 2 hours for enzymolysis;

[0020] (3) After the enzymolysis is finished, add 50% ethanol according to the solid-liquid ratio of dandelion dry powder at 1:10g / ml, and place it in a constant temperature water bath to reflux for 2 hours, then add the dandelion dry powder at a solid-liquid ratio of 1:3g / ml, the concentration is 10g / L chitosan solution, after standing for 2h, carry out suction filtration and concentrate to get product.

Embodiment 2

[0022] The method for efficiently extracting chlorogenic acid in dandelion comprises the following steps:

[0023] (1) Put the dandelion dry powder in a clean container, add deionized water solvent to dissolve the dandelion dry powder according to the material-to-liquid ratio of 1:11g / ml, and set aside;

[0024] (2) Add an enzyme liquid to the dandelion dry powder solution, the ratio of the added enzyme liquid to the dandelion dry powder is 1:2g / ml, and place it at a temperature of 42°C for 2.5 hours for enzymatic hydrolysis;

[0025] (3) After the enzymolysis is finished, add 50% ethanol according to the solid-liquid ratio of dandelion dry powder at 1:10g / ml, and place it in a constant temperature water bath to reflux for 2 hours, then add the dandelion dry powder at a solid-liquid ratio of 1:3g / ml, the concentration is 10g / L chitosan solution, after standing for 2h, carry out suction filtration and concentrate to get product.

Embodiment 3

[0027] The method for efficiently extracting chlorogenic acid in dandelion comprises the following steps:

[0028] (1) Put the dandelion dry powder in a clean container, add deionized water solvent to dissolve the dandelion dry powder according to the material-to-liquid ratio of 1:12g / ml, and set aside;

[0029] (2) Add an enzyme solution to the dandelion dry powder solution, the ratio of the added enzyme solution to the dandelion dry powder is 1:3g / ml, and place it at a temperature of 45°C for 3 hours for enzymolysis;

[0030] (3) After the enzymolysis is finished, add 50% ethanol according to the solid-liquid ratio of dandelion dry powder at 1:10g / ml, and place it in a constant temperature water bath to reflux for 2 hours, then add the dandelion dry powder at a solid-liquid ratio of 1:3g / ml, the concentration is 10g / L chitosan solution, after standing for 2h, carry out suction filtration and concentrate to get product.

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PUM

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Abstract

The invention provides a method for efficiently extracting chlorogenic acid from dandelion. The method comprises (1) putting dandelion dry powder in a container and adding deionized water to dissolve the dandelion dry powder, (2) adding an enzyme solution into the dandelion dry powder solution and carrying out enzymolysis at a temperature of 40-50 DEG C for 2-4h, and (3) after the enzymolysis, adding 50% of ethanol into a constant temperature water bath pot, carrying out backflow, then adding a chitosan solution into the enzymolysis product, carrying out standing for 2h and carrying out suction filtration concentration to obtain a product. The enzymolysis-alcohol extraction method changes the existing single water extraction or alcohol extraction method. Under the optimal extraction conditions of an enzyme liquid use ratio of 1: 3 g / ml, a material-liquid ratio of 1: 12 g / ml, an enzymolysis temperature of 45 DEG C and enzymolysis time of 3h, an extraction rate of chlorogenic acid in dandelion is 6.55 mg / g. The extract can be used for improving quality of a cigarette so that maximum benefits of dandelion are obtained. The method is simple, can be operated easily and is suitable for industrial production.

Description

technical field [0001] The invention relates to a method for extracting natural products, in particular to a method for efficiently extracting chlorogenic acid in dandelion. Background technique [0002] Dandelion, also known as Huanghuadiding, Popoding, Huahualang, etc. Asteraceae, a perennial herb. The dandelion plant contains various healthy nutrients such as dandelion alcohol, dandelion, choline, organic acid, and inulin. Nature and flavor are sweet, slightly bitter, cold. Return liver, stomach warp. Diuretic, laxative, anti-jaundice, choleretic and other effects. Treatment of heat, bloated, sores, internal hemorrhoids, red eyes and swelling, damp heat, jaundice, urination, phlegm, astringent pain, hemorrhoids, phlegm, phlegm, toothache, red eyes, sore throat, lung abscess, Intestinal abscess, damp-heat jaundice, hot dripping pain. Treatment of acute mastitis, lymphadenitis, scrofula, furunculosis, acute conjunctivitis, cold and fever, acute tonsillitis, acute bron...

Claims

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Application Information

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IPC IPC(8): C07C69/732C07C67/48
CPCC07C67/48C07C69/732
Inventor 马健
Owner 马健
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