Method for efficiently extracting chlorogenic acid from dandelion
A technology of dandelion and chlorogenic acid, applied in the separation/purification of carboxylic acid esters, organic chemistry, etc., can solve the problems of low economic value of dandelion, low utilization rate of dandelion, and single extraction method, so as to improve the sweetness and ease of operation , The effect of low extraction cost
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[0016] Example 1
[0017] The method for efficiently extracting chlorogenic acid from dandelion includes the following steps:
[0018] (1) Put the dry dandelion powder in a clean container, add deionized water solvent to dissolve the dry dandelion powder at a material-to-liquid ratio of 1:10 / ml, and set aside;
[0019] (2) Add the enzyme solution to the dandelion dry powder solution, the added enzyme solution and the dandelion dry powder have a material-liquid ratio of 1:2g / ml, and place the enzyme solution at a temperature of 40°C for 2 hours;
[0020] (3) After the enzymolysis is completed, add 50% ethanol at a material-liquid ratio of 1:10g / ml to the dry dandelion powder, put it in a constant temperature water bath for 2 hours, and add a material-liquid ratio of 1:3g to the dry dandelion powder. / ml, a concentration of 10g / L chitosan solution, after standing for 2h, suction filtration and concentration to obtain the product.
Example Embodiment
[0021] Example 2
[0022] The method for efficiently extracting chlorogenic acid from dandelion includes the following steps:
[0023] (1) Put the dry dandelion powder in a clean container, add deionized water solvent to dissolve the dry dandelion powder at a material-to-liquid ratio of 1:11g / ml, and set aside;
[0024] (2) Add the enzyme solution to the dandelion dry powder solution, the added enzyme solution and the dandelion dry powder have a material-liquid ratio of 1:2g / ml, and place the enzyme solution at 42°C for 2.5 hours;
[0025] (3) After the enzymolysis is completed, add 50% ethanol at a material-liquid ratio of 1:10g / ml to the dry dandelion powder, put it in a constant temperature water bath for 2 hours, and add a material-liquid ratio of 1:3g to the dry dandelion powder. / ml, a concentration of 10g / L chitosan solution, after standing for 2h, suction filtration and concentration to obtain the product.
Example Embodiment
[0026] Example 3
[0027] The method for efficiently extracting chlorogenic acid from dandelion includes the following steps:
[0028] (1) Put the dry dandelion powder in a clean container, add deionized water solvent to dissolve the dry dandelion powder at a material-to-liquid ratio of 1:12g / ml, and set aside;
[0029] (2) Add the enzyme solution to the dandelion dry powder solution, the added enzyme solution and the dandelion dry powder have a material-liquid ratio of 1:3g / ml, and place the enzyme solution at a temperature of 45° C. for 3 hours;
[0030] (3) After the enzymolysis is completed, add 50% ethanol at a material-liquid ratio of 1:10g / ml to the dry dandelion powder, put it in a constant temperature water bath for 2 hours, and add a material-liquid ratio of 1:3g to the dry dandelion powder. / ml, a concentration of 10g / L chitosan solution, after standing for 2h, suction filtration and concentration to obtain the product.
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