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A kind of method for extracting protein from soil mold slag

A technology of protein and fungus residue, which is applied in the field of solid waste treatment and resource recovery, can solve problems that have not been reported before, and achieve the effects of resource utilization, low cost and convenient operation

Active Publication Date: 2021-06-29
HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no reports on the extraction of protein from antibiotic residues at home and abroad.

Method used

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  • A kind of method for extracting protein from soil mold slag
  • A kind of method for extracting protein from soil mold slag

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Oxytetracycline slag homogenized pretreatment and acid-heat combined treatment

[0022] Oxytetracycline slag Oxytetracycline slag homogenization pretreatment: adjust the moisture content of the original bacterial slag (moisture content ≤ 60-80) of the pharmaceutical factory to 90wt%~99wt%, put it in a beater and stir for 8 minutes, Perform homogenization pretreatment;

[0023] Acid-heat combined treatment of oxytetracycline slag: adjust the pH of the pre-treated bacterial slag solution to 1-3, stir evenly, put it into a constant temperature reactor, adjust the reaction temperature to 80 ℃ ~ 120 ℃, the reaction time is 2h ~ 6h, The bacteria residue cells are broken and the protein is dissolved.

[0024] Extraction and detection of protein solution: put the mixed solution of bacterial residue after acid-heat treatment into a centrifuge, adjust the speed of the centrifuge to 4000r / min, control the centrifugation time to 10min, put the protein extract in the cent...

Embodiment 2

[0028] Example 2 Separation of soluble protein extract

[0029] Separation of soluble protein extract: After acid-heat combined treatment, the bacterial residue solution was cooled to room temperature, and then put into a centrifuge for solid-liquid centrifugation. The protein extract in the centrifuge tube and the remaining residue are centrifuged to obtain a soluble protein extract. In the experiment, the rotational speed of the centrifuge and the centrifugation time can be adjusted by themselves within the scope of the claims, and the experimental results are basically the same under different centrifugation conditions.

Embodiment 3

[0030] Example 3 Isoelectric point method precipitation of protein and separation of protein

[0031] When the treatment conditions were moisture content of 94%, reaction pH of 2.0, reaction temperature of 120 °C, and reaction time of 2 h, the protein dissolution concentration in the bacterial residue was 6376.93 mg / L, and the isoelectric point method was used for protein precipitation and separation.

[0032] Protein precipitation by isoelectric point method: adjust the pH of the soluble protein extract obtained by centrifugation, use the isoelectric point method to precipitate and purify the protein, and adjust the pH of the centrifuged supernatant to 3.0, 4.0, 5.0, 6.0 with 1 mol / L NaOH solution , 7.0, 8.0, 9.0, the reaction precipitation time is 8 minutes;

[0033] Protein separation: put the precipitated protein extract into a centrifuge for solid protein purification and separation, control the speed of the centrifuge to 4000r / min, control the centrifugation time to 18mi...

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Abstract

The invention belongs to the field of solid waste treatment and resource recovery, and specifically relates to a method for extracting protein by crushing and digesting oxytetracycline slag with an acid / heat combination method. The method comprises the following steps: homogeneous pretreatment of oxytetracycline slag, combined acid-heat treatment of oxytetracycline slag, separation of soluble protein extract, isoelectric point method precipitation protein and protein separation. Combined acid-heat process, the bacterial residue is lysed to dissolve the protein, so as to realize the separation and purification of protein solids from the bacterial residue, and extract protein from the oxytetracycline bacterial residue, which can realize the recycling and reduction treatment of the bacterial residue .

Description

technical field [0001] The invention belongs to the field of solid waste treatment and disposal and resource recovery, and in particular relates to a method for extracting protein by crushing and digesting oxytetracycline slag by an acid / heat combined method. Background technique [0002] my country is the world's largest producer and exporter of antibiotic APIs. According to statistics, the production of antibiotics in 2013 reached 121,200 tons, including industrial salts of penicillin, cephalosporins, oxytetracycline and streptomycin. Even if the production of 1t of antibiotics produces 8t of bacterial residue (actually it may exceed this value), the annual production of antibiotics in my country will reach more than 1 million tons. [0003] Antibiotic bacterial residue is the remaining fermentation residue after the production of antibiotics, and was included in the "National List of Hazardous Wastes" implemented in 2008, and is prohibited from being used as animal feed, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K1/30C07K1/14
CPCC07K1/14C07K1/30
Inventor 李再兴李贵霞王贺飞钟为章秦学
Owner HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY