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Method for extracting chitosan from oyster shells

A technology for oyster shells and chitosan, which is applied in the field of marine biological activity extraction, can solve the problems of unclean processing, troublesome pretreatment, and difficulty in recycling, and achieves the effect of shortened process time and white color.

Inactive Publication Date: 2017-05-31
广西还珠海洋生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still some problems that cannot be ignored in the preparation of chitin from shrimp and crab shells. First, except for some large-scale seafood processing enterprises, shrimp and crab shells are difficult to purchase in a centralized manner. Generally, after being eaten by families or hotels, they are discarded as garbage. It is difficult to recycle; the second is that shrimp and crab shells contain a large amount of symbionts such as protein and fat, and the pretreatment is more troublesome. The treatment is not clean or completely dry, and it is easy to mold and deteriorate during storage.
It is because of such reasons that the full utilization of shrimp and crab shells is greatly restricted.
Chinese patent CN103936884A, a method for extracting chitin from shrimp and crab shells, is the applicant's prior application, but this method is not effective for processing oyster shell raw materials
Soochow University paper, Liu Hongxi, Extraction of oyster shell chitin and its derivatives in printing and dyeing, mentions the extraction of oyster shell chitin, but the focus is on the application of the obtained chitin in printing and dyeing, and its production process is Small laboratory tests, such as filtering with filter paper, many process steps and parameters are not suitable for industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A method for extracting chitosan from oyster shells, comprising the steps of:

[0028] 1) Cleaning: washing the discarded oyster shells with clear water to remove silt and impurities on the surface of the oyster shells;

[0029] 2) Drying: 100 kg of oyster shells obtained in step 1) were dried and pulverized with a ball mill until the particle size was 80 mesh to obtain oyster shell coarse powder;

[0030] 3) Low-temperature pulverization: The oyster shell coarse powder obtained in step 2) is subjected to low-temperature freezing at 0°C and low-temperature sublimation drying in sequence, and then uses liquid nitrogen as a grinding medium, and then uses an ultra-low temperature pulverizer to carry out low-temperature pulverization at -80°C;

[0031] 4) High-pressure homogenization: Add the material obtained in step 3) to 5 times the weight of pure water and mix evenly, then add it to a high-pressure homogenizer for processing, 10000psi, 20min;

[0032] 5) Acid treatment...

Embodiment 2

[0035] A method for extracting chitosan from oyster shells, comprising the steps of:

[0036] 1) Cleaning: washing the discarded oyster shells with clear water to remove silt and impurities on the surface of the oyster shells;

[0037] 2) Drying: 100 kg of oyster shells obtained in step 1) were dried and pulverized with a ball mill until the particle size was 100 mesh to obtain coarse powder of oyster shells;

[0038] 3) Low-temperature pulverization: the oyster shell coarse powder obtained in step 2) is subjected to low-temperature freezing at -10°C and low-temperature sublimation drying in sequence, and then liquid nitrogen is used as the grinding medium, and low-temperature pulverization is performed at -100°C using an ultra-low temperature pulverizer;

[0039] 4) High-pressure homogenization: Add the material obtained in step 3) to 6 times the weight of pure water and mix evenly, then add it to a high-pressure homogenizer for processing, 15000psi, 15min;

[0040]5) Acid t...

Embodiment 3

[0043] A method for extracting chitosan from oyster shells, comprising the steps of:

[0044] 1) Cleaning: washing the discarded oyster shells with clear water to remove silt and impurities on the surface of the oyster shells;

[0045] 2) Drying: 100 kg of oyster shells obtained in step 1) were dried and pulverized with a ball mill until the particle size was 80 mesh to obtain oyster shell coarse powder;

[0046] 3) Low-temperature crushing: the oyster shell coarse powder obtained in step 2) is subjected to low-temperature freezing at -18°C and low-temperature sublimation drying in sequence, and then liquid nitrogen is used as the grinding medium, and low-temperature crushing is carried out at -120°C by using an ultra-low temperature grinder;

[0047] 4) High-pressure homogenization: add the material obtained in step 3) to 5 times the weight of pure water and mix evenly, then add it to a high-pressure homogenizer for processing, 18000psi, 10min;

[0048] 5) Acid treatment: ad...

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PUM

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Abstract

The invention discloses a method for extracting chitosan from oyster shells. The method includes the steps of firstly, using clean water to clean waste oyster shells to remove silt and impurities on the surfaces of the oyster shells; secondly, drying and crushing the oyster shells obtained in the first step to obtain oyster shell powder; thirdly, sequentially performing low-temperature freezing and low-temperature lyophilization, and then using liquid nitrogen as the grinding medium to perform low-temperature crushing under the temperature from minus 80 DEG C to minus 120 DEG C by using an ultralow-temperature crusher; fourthly, adding purified water into materials obtained in the third step, evenly mixing, and processing in a high-pressure homogenizing machine; fifthly, performing acid treatment, to be more specific, soaking materials obtained in the fourth step into diluted hydrochloric acid, washing until neutrality is achieved, centrifuging, and drying to obtain chitin; sixthly, performing deacetylation, and drying to obtain the chitosan. The method has the advantages that the whole working procedure time is shortened, black impurities in the intermediate working procedure are avoided, and the obtained chitosan is pure white.

Description

technical field [0001] The invention relates to the technical field of marine biological activity extraction, in particular to a method for extracting chitosan from oyster shells. Background technique [0002] Chitosan is obtained by deacetylating chitin under strong alkaline conditions. Chitin, also known as chitin, was discovered by French scholar Braconno in 1811, and was extracted from crustacean shells by Ogier in 1823, and named CHITIN, translated as chitin. Chitin is composed of N-acetyl-2-amino-2-deoxy-D-glucose linked by β-1,4 glycosidic bonds, which is the glycan of N-acetyl-D-glucosamine. Because chitin will take off part of the acetyl group during the production process, the commercialized chitin is a copolymer of N-acetyl-D-glucosamine and D-glucosamine. [0003] Chitin is the second largest biological resource after cellulose in nature. It is estimated that the annual biosynthesis of chitin is as high as 10 billion tons. It is one of the most abundant organic...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/003C08B37/0003
Inventor 归三岷
Owner 广西还珠海洋生物科技有限公司