A kind of mycelium collection method of filamentous fungal host infection stage

Abstract

The invention provides a mycelium collection method at a filamentous fungus host infection stage. The method comprises steps as follows: (1) sterilized host leaves are alternately placed in a wide-neck bottle, the angle formed by the leaves and the bottle bottom is 30-60 degrees, and a wetted carrier material is placed at the bottom of the wide-neck bottle; hosts comprise at least one of gramineous plants, leguminous plants, linaceous plants, feverfew and cruciferae plants; the carrier material comprises at least one of cotton and filter paper; (2) the host leaves in the wide-neck bottle are inoculated with cultured fungus mycelia, and mycelium groups are uniformly dispersed; fungi comprise rhizoctonia solani and magnaporthe oryzae. (3) a substance obtained from step (2) is placed in an incubator for culture, and mycelia are collected. With the adoption of the method, fungus mycelia can be collected efficiently, the pollution problem in routine techniques is solved, time and labor are saved, and the cost is low; meanwhile, a large quantity of mycelia is obtained, the mycelium quality is stable, and follow-up scientific research is facilitated.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a method for collecting hyphae in the infection stage of a filamentous fungal host. Background technique [0002] At present, there are few studies on transcriptome sequencing and other aspects in the fungal infection stage, the main reason is that the collection of hyphae is difficult. Using conventional methods to collect mycelium, the amount of collection is small and time-consuming, so that many studies tend to do mixed sequencing of hosts and fungi, but this method makes the efficiency of clean reads compared to reference sequences in fungal transcriptome data relatively high Low, limiting fungal research by bioinformatics methods. [0003] In the research field of fungal hyphae collection, the method that can realize a large number of collections is mainly liquid culture collection. CN104480022 A discloses a method for collecting mycelium by filtration using a st...

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Problems solved by technology

However, the inventors have found that since some miscellaneous bacteria can also grow in the medium used, this method is also difficult to solve the problem of bacterial contamination

More importantly, this method needs to carefully insert the host blade into the solid medium, which is very time-consuming, and the resulting mycelium yield of this method is very low (each petri dish only obtains less than 0.04g of mycelium) , also cannot solve the problem of "less mycelium collection and time-consuming" faced by the prior art

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