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Culture medium suitable for bacterial cellulose fermentation

A technology of bacterial cellulose and culture medium, applied in the field of fermentation, can solve the problems of restricting the popularization and application of bacterial cellulose, low bacterial cellulose yield, increasing unit production cost, etc. , the effect of low cost

Active Publication Date: 2017-05-31
BEIJING GUANLAN TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current medium mainly has the following disadvantages: 1) The raw material preparation process is complicated and consumes a lot of manpower and material resources
2) Raw materials are subject to geographical restrictions, which is not conducive to promotion
3) The yield of bacterial cellulose is low, which increases the unit production cost. The high production cost limits the promotion and application of bacterial cellulose. Currently, bacterial cellulose is mainly limited to the product field with high added value
[0008] At present, there is no report on the application of whole corn flour, soybean flour and soybean meal to the fermentation of bacterial cellulose

Method used

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  • Culture medium suitable for bacterial cellulose fermentation

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Effect test

Embodiment 1

[0037] Embodiment 1 A kind of culture medium suitable for bacterial cellulose fermentation and the method for producing bacterial cellulose by fermentation

[0038] Seed culture medium: based on 1000 parts, 15 parts of corn flour, 5 parts of soybean flour, 5 parts of white sugar, 1 part of tryptone, 2 parts of yeast extract, 3 parts of potassium dihydrogen phosphate, 1 part of magnesium sulfate, and the balance is Water, adjust the pH to 6.8, and sterilize at 121°C for 20 minutes;

[0039] Fermentation medium: based on 1000 parts, 20 parts of corn flour, 6 parts of soybean meal powder, 5 parts of white sugar, 2 parts of potassium dihydrogen phosphate, 2 parts of magnesium sulfate, 1 part of calcium chloride, and the balance is water, adjust the pH to 6.8, sterilize at 121°C for 20 minutes;

[0040] The corn flour is crushed from whole corn to 60 mesh or below; the soybean powder is crushed from soybean grains to 60 mesh or below; the soybean meal powder is soaked soybean meal...

Embodiment 2

[0044] Embodiment 2 A kind of culture medium suitable for bacterial cellulose fermentation and the method for producing bacterial cellulose by fermentation

[0045] Seed culture medium: based on 1000 parts, 10 parts of corn flour, 12 parts of soybean flour, 6 parts of white sugar, 1 part of yeast extract, 1 part of tryptone, 3 parts of potassium dihydrogen phosphate, 1 part of magnesium sulfate, and the balance is water, adjust the pH to 6.8, and sterilize at 121°C for 20 minutes.

[0046] Fermentation medium: based on 1000 parts, 14 parts of corn flour, 8 parts of soybean meal powder, 10 parts of white sugar, 2 parts of potassium dihydrogen phosphate, 2 parts of magnesium sulfate, 1 part of calcium chloride, and the balance is water, adjust the pH to 6.8, sterilize at 121°C for 20 minutes.

[0047] Seed preparation: In the ultra-clean workbench, the slant strains were transferred to the seed medium, and cultivated in a constant temperature shaker at 25°C and 150rpm for 48 ho...

Embodiment 3

[0051] Embodiment 3 A kind of culture medium suitable for bacterial cellulose fermentation and the method for producing bacterial cellulose by fermentation

[0052] Preparation of seed culture medium: based on 1000 parts, 5 parts of corn flour, 10 parts of soybean flour, 7 parts of white sugar, 2 parts of tryptone, 2 parts of yeast extract, 3 parts of potassium dihydrogen phosphate, 1 part of magnesium sulfate, and the balance For water, adjust the pH to 6.8, and sterilize at 121°C for 20 minutes.

[0053] Fermentation medium preparation: based on 1000 parts, 10 parts of corn flour, 14 parts of soybean meal powder, 12 parts of white sugar, 2 parts of potassium dihydrogen phosphate, 2 parts of magnesium sulfate, 1 part of calcium chloride, and the balance is water to adjust the pH Sterilize at 6.8,121°C for 20 minutes.

[0054] Seed preparation: In the ultra-clean workbench, the slant strains were transferred to the seed culture medium, cultivated in a constant temperature sha...

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Abstract

The invention belongs to the technical field of fermentation, and specifically relates to a culture medium suitable for bacterial cellulose fermentation. The culture medium takes corn flour, soybean meal and bean pulp powder as main materials, so the cost is reduced; furthermore, the raw materials are cheap, easily available, rich and comprehensive in nutrition and simple in processing process, so the cost is reduced by 5 to 7 times compared with that of the conventional bacterial cellulose fermentation culture medium; the bacterial cellulose fermentation yield of 24 g / L or above can be finished through cultivation for 136 to 144 hours, so the production efficiency of cellulose is improved.

Description

technical field [0001] The invention belongs to the technical field of fermentation, and in particular relates to a culture medium suitable for bacterial cellulose fermentation. [0002] technical background [0003] Bacterial cellulose is a general term for the cellulose synthesized and secreted by a certain group of microorganisms (bacteria). In industrial production, Acetobacter xylinum is often used as the bacterial cellulose fermentation strain. As a new type of biological material, bacterial cellulose has attracted extensive attention due to its unique properties. Bacterial cellulose has high purity, ultrafineness, water permeability, good biocompatibility, degradability and biosynthesis controllability. Broad application prospects. [0004] Microbial fermentation is a complex and systematic biological process, which is affected by many factors, such as medium composition, ratio, cultivation temperature, and cultivation method. Among them, the composition and ratio o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12R1/02
CPCC12P19/04
Inventor 朱翠玲段得振郝华璇
Owner BEIJING GUANLAN TECH