Automatic detection system of circulating tumor cells based on Raman spectrum

Abstract

The invention provides an automatic detection system of circulating tumor cells based on a Raman spectrum. The system is composed of a blood sample processing module, a circulating tumor cell detection module, a data processing module and a result output module, wherein the blood sample processing module is mainly based on an enriching effect on circulating tumor cells of a microporous filter chip, the circulating tumor cell detection includes optical microimaging detection and micro-Raman spectrum detection, and detection data are compared with a database, so that information such as the quantity, kind, period and the like of the circulating tumor cells (CTC) are obtained ultimately.The system has the advantages of high integration level, simplicity in operation and good specificity of the Raman spectrum, a detection result can be obtained when a blood sample is directly put in the system, and the system can be widely applied to the fields of clinical blood test, early diagnosis and prognosis monitoring of cancer, basic scientific research and the like.

Description

technical field [0001] The invention relates to an automatic detection system for circulating tumor cells, in particular to an automatic system for detecting circulating tumor cells in blood based on Raman spectroscopy. Background technique [0002] Circulating tumor cells (CTCs) are the main cause of cancer metastasis, with a lethal rate as high as 90%. To detect CTCs from a patient's blood, only a certain amount of peripheral blood needs to be drawn, and no pathological sections are required. Not only does it cause little damage to the patient, but it can also accurately grasp the patient's condition and judge the risk of cancer metastasis, which is of great value for the prognosis of early cancer. However, the content of CTCs in the blood is very small, much lower than that of normal red blood cells and white blood cells. Traditional methods are mainly immunoassays, including immunomagnetic beads, immunofluorescence, etc. Surface antigens are easy to lose, and some CTCs ...

AI Technical Summary

Problems solved by technology

However, the content of CTCs in the blood is very small, much lower than that of normal red blood cells and white blood cells. Traditional methods are mainly immunoassays, including immunomagnetic beads, immunofluorescence, etc. Surface antigens are easy to lose, and some CTCs may even form stem cells. Therefore, immunological methods are very easy to miss detection, and there are also false positive binding problems, resulting in a low detection rate of CTCs by traditional immunological methods. The types are limited, resulting in limited types of CTCs that can be detected by this method

In addition, cancer cell immune antibodies are expensive, resulting in high testing costs for patients

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