Composition based on a stabilized solution of active ingredients
A technology of active substances and compositions, applied in the field of medicine
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Embodiment 1
[0192] Preparation of stable solutions and pharmaceutical compositions based on the solutions
[0193] Add 800ml of distilled water to the flask. 16g of sodium succinate, 50mg of succinic acid, and 1.2g of sodium chloride were sequentially added to the flask and mixed on a magnetic stirrer (600rpm) for 5-10min until the ingredients were completely dissolved. Then, 200 mg of dicathocin was added to the solution under constant stirring until it was completely dissolved, and molten high-molecular-weight hyaluronic acid (401kDa-2,500,000Da) was added and stirred on a magnetic stirrer until the high-molecular-weight hyaluronic acid was completely hydrated (dissolved).
[0194] Subsequently, the pH of the obtained solution was adjusted to 7.3 with succinic acid, if necessary, and finally the prepared solution was made up to 1000 ml with purified water.
Embodiment 2
[0196] Preparation of stable solutions
[0197] Repeat the steps of Example 1, except that sodium hyaluronate is used instead of high molecular weight hyaluronic acid.
Embodiment 3
[0199] Preparation of gel formulations of pharmaceutical compositions based on stable solutions
[0200] Add 800ml of distilled water to the flask. 200mg of dicathoxine, 1.42g of sodium lactate, 360mg of lactic acid, and 30g of glycerin were sequentially added to the flask and mixed on a magnetic stirrer (600rpm) for 5-10min until the ingredients were completely dissolved. Then, 5 g of high molecular weight hyaluronic acid (401 kDa-2,500,000 Da) was added to the solution under constant stirring and stirred on a magnetic stirrer until the hyaluronic acid was completely hydrated (dissolved). 30 g of hydroxypropylmethylcellulose was added to the solution, and the volume of the mixed solution was adjusted to 1000 ml with purified water and mixed. If necessary, add lactic acid or sodium lactate to adjust the pH of the solution to 7.315.
[0201] The preparation method based on dicathoxine and low-molecular hyaluronic acid stable solution was tested in a large range of component r...
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