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Polymer tissue engineering scaffold porous material with good pore connectivity and preparation method thereof

A technology of hole connectivity and tissue engineering, applied in medical science, prosthesis, etc., can solve problems such as poor connectivity and limited cell culture, and achieve the effect of low cost, good connectivity and high safety

Active Publication Date: 2019-11-12
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although porous scaffolds with appropriate pore sizes can be prepared by this method, the porous scaffolds produced by this method have poor connectivity between pores, which limits cell culture in tissue engineering.

Method used

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  • Polymer tissue engineering scaffold porous material with good pore connectivity and preparation method thereof
  • Polymer tissue engineering scaffold porous material with good pore connectivity and preparation method thereof
  • Polymer tissue engineering scaffold porous material with good pore connectivity and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Weigh two kinds of polymers according to the volume ratio of polylactic acid (PLA): polyvinylpyrrolidone (PVP) = 50: 50, premix the two kinds of polymers at room temperature to obtain a premix, and then premix the The mixture was mixed in a micro extruder at 180°C under normal pressure for 8 minutes at a speed of 80rpm to obtain a melt blend of the two polymers;

[0027] (2) Mold the molten blend obtained in step (1) on a vacuum molding machine, and rapidly cool and shape it to obtain a PLA / PVP flake blend green body. The molding temperature is 180° C., and the molding pressure is 10 MPa , molding time is 5min;

[0028] (3) Place the flake-shaped blended body obtained in step (2) in a high-pressure reactor, feed foaming gas carbon dioxide and raise the temperature to 150°C and pressurize to 20MPa (under this condition, the carbon dioxide has transformed into a supercritical fluid ) heat preservation and pressure for 4 hours to coarsen the flake blended body, and ma...

Embodiment 2

[0031] (1) Weigh two kinds of polymers according to the volume ratio of polylactic acid (PLA):polycaprolactone (PCL)=50:50, and pre-mix the two kinds of polymers at room temperature to obtain a premix, and then premix The mixture was mixed for 5 minutes at 50 rpm in a micro extruder at normal pressure and 180° C. to obtain a melt blend of the two polymers;

[0032] (2) Mold the molten blend obtained in step (1) on a vacuum molding machine, and rapidly cool and shape it to obtain a PLA / PCL flake blend body. The molding temperature is 180° C., and the molding pressure is 10 MPa , molding time is 3min;

[0033](3) Place the sheet-like blend green body obtained in step (2) in a high-pressure reactor, introduce foaming gas carbon dioxide and raise the temperature to 120°C and pressurize to 20MPa (under this condition, the carbon dioxide has transformed into supercritical Fluid) heat preservation and pressure for 1h to coarsen the flake blend body, then heat preservation and pressu...

Embodiment 3

[0036] (1) Weigh the two polymers according to the volume ratio of polylactic-co-glycolic acid (PLGA): polyethylene glycol (PEG) = 60: 40, and pre-mix the two polymers at room temperature to obtain a pre-mixed Then the premix was mixed at normal pressure and 140°C in a micro extruder at a speed of 80rpm for 8min to obtain a melt blend of the two polymers;

[0037] (2) Mold the molten blend obtained in step (1) on a vacuum molding machine, and rapidly cool and shape it to obtain a PLGA / PEG flake blend green body. The molding temperature is 140° C., and the molding pressure is 10 MPa , molding time is 3min;

[0038] (3) Place the sheet-like blend green body obtained in step (2) in a high-pressure reactor, introduce foaming gas carbon dioxide, heat up to 100°C, and pressurize to 20MPa (under this condition, the carbon dioxide has transformed into supercritical Fluid) heat preservation and pressure for 2 hours to coarsen the flake blend body, then heat preservation and pressure f...

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Abstract

A preparation method of a polymeric tissue engineering porous scaffold material with good pore connectivity: (1) melting and blending two thermoplastic polymers to obtain a melted blend; (2) pressing the melted blended body into a blended green body; (3) Place the blended body in a high-pressure reaction kettle, feed foaming gas, heat up to 50-110°C, pressurize to 5-25MPa, keep the pressure for 0.5-4 hours to coarsen the blended body, and then At a temperature of 40-150°C and a pressure of 8-25 MPa, the supercritical fluid in the high-pressure reactor is saturated in the blended body, and then the temperature is kept constant at a rate of 5-15 MPa / s. The pressure is reduced to normal pressure to make the blended green body foam, and after cooling and shaping, a foamed green body is obtained; (4) the foamed green body is immersed in an etching solution for etching. The method of the invention can further improve the connectivity between the pores of the polymer tissue engineering scaffold material.

Description

technical field [0001] The invention belongs to the fields of foaming polymer material processing and biomedicine, in particular to a polymer tissue engineering porous support material and a preparation method thereof. Background technique [0002] Tissue engineering is an emerging discipline that has emerged in recent years. It cultivates normal tissue cells in vitro and attaches them to a scaffold material with good cytocompatibility and biodegradability to form a cell-scaffold complex. The complex is implanted into the body tissue or the damaged part of the organ, and a new tissue structure is gradually formed in the cell body, while the scaffold material is gradually degraded and absorbed by the organism, so as to achieve the purpose of repairing and rebuilding the tissue body. There are three core elements in tissue engineering: seed cells, tissue engineering scaffold materials, and growth factors. Scaffold materials play an important role in tissue engineering, becaus...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/18A61L27/56
CPCA61L27/18A61L27/56C08L67/04
Inventor 廖霞叶建刚黄二波李光宪
Owner SICHUAN UNIV
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