Method for preparing oxidation-resistant active peptides from crab shells by double-enzyme multistep enzymolysis

A step-by-step enzymatic hydrolysis technology for antioxidant active peptides, which is applied to the preparation method of peptides, chemical instruments and methods, peptides, etc., can solve problems such as pollution of resources and waste, and achieve stable and controllable processes, simple process steps, and increased The effect of economic value

Inactive Publication Date: 2017-06-06
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Crabs will produce a lot of leftovers after processing, such as crab legs, crab back shells, etc. Due to technical and understanding reasons, most of these shrimp and crab shells are often discarded at will, causing great pollution and waste of resources
However, the processing and utilization of crab shells by domestic production enterprises is still in its infancy, and most of the existing processing and utilization methods are used for the production of chitin, except for a small part of coarse crushing as raw materials for fishmeal processing, and most chitin production enterprises face environmental protection pressure and stop. Production

Method used

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  • Method for preparing oxidation-resistant active peptides from crab shells by double-enzyme multistep enzymolysis

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Raw material pretreatment: wash the crab shells with water, dry them in the air, and pulverize them with a pulverizer to obtain crab shell powder for later use;

[0019] (2) Dual-enzyme enzymatic hydrolysis: mix crab shell powder and water at a mass ratio of 1:5, adjust the pH to 2.0, keep warm at 30°C for 10 minutes, add 0.1% of the mass of crab shell powder and 20,000 U / g of enzyme activity Acid protease, heat preservation enzymolysis for 6 hours under stirring condition, rise to 95°C to inactivate enzyme for 15min, cool to 50°C, adjust pH to 6.5, add flavor protease with 0.1% of crab shell powder mass and enzyme activity ≥30000U / g, in Under the condition of stirring, incubate the enzymatic hydrolysis at 50°C for 2 hours, then raise the temperature to 95°C to inactivate the enzyme for 10 minutes to obtain the enzymatic hydrolysis solution;

[0020] (3) Centrifugal separation: perform high-speed centrifugation on the enzymatic hydrolysis solution, and take the supe...

Embodiment 2

[0025] (1) Raw material pretreatment: wash the crab shells with water, dry them in the air, and pulverize them with a pulverizer to obtain crab shell powder for later use;

[0026] (2) Double-enzyme hydrolysis: mix crab shell powder and water at a mass ratio of 1:10, adjust the pH to 3.0, keep warm at 40°C for 20 minutes, add 0.3% crab shell powder mass and 50,000 U / g enzyme activity Acid protease, heat preservation enzymolysis for 8 hours under stirring conditions, rise to 100°C to inactivate enzyme for 20min, cool to 60°C, adjust pH to 7.5, add flavor protease with 0.3% of crab shell powder mass and enzyme activity ≥30000U / g, in Under the condition of stirring, incubate the enzymatic hydrolysis at 60°C for 4 hours, then raise the temperature to 100°C to inactivate the enzyme for 20 minutes to obtain the enzymatic hydrolysis solution;

[0027] (3) Centrifugal separation: perform high-speed centrifugation on the enzymatic hydrolysis solution, and take the supernatant to obtain...

Embodiment 3

[0032] (1) Raw material pretreatment: wash the crab shells with water, dry them in the air, and pulverize them with a pulverizer to obtain crab shell powder for later use;

[0033] (2) Double-enzyme enzymatic hydrolysis: mix crab shell powder and water at a mass ratio of 1:7, adjust the pH to 2.5, incubate at 35°C for 15 minutes, add 0.2% crab shell powder mass and 30,000 U / g enzyme activity Acid protease, heat preservation enzymolysis for 7 hours under stirring condition, rise to 98°C to inactivate enzyme for 17min, cool to 55°C, adjust pH to 7.2, add flavor protease with 0.2% of crab shell powder mass and enzyme activity ≥30000U / g, in Under the condition of stirring, incubate the enzymatic hydrolysis at 55°C for 3 hours, then raise the temperature to 97°C to inactivate the enzyme for 15 minutes to obtain the enzymatic hydrolysis solution;

[0034] (3) Centrifugal separation: perform high-speed centrifugation on the enzymatic hydrolysis solution, and take the supernatant to o...

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Abstract

The invention discloses a method for preparing oxidation-resistant active peptides from crab shells by double-enzyme multistep enzymolysis. The method comprises the following steps: (1) raw material pretreatment; (2) double-enzyme enzymolysis; (3) centrifugal separation; (4) membrane separation; and (5) vacuum freeze drying. By using the crab shells as the raw material, the crab shells are pulverized, and subjected to double-enzyme multistep enzymolysis, centrifugal separation, membrane separation and vacuum freeze drying to obtain the solid crab shell oxidation-resistant peptides. The method has the advantages of simple technical steps, stable and controllable process, energy saving, high efficiency and environment friendliness, implements comprehensive utilization on crab processing waste resources, enhances the application value of the crabs, and effectively increases the economic value of the crabs.

Description

technical field [0001] The invention relates to the technical field of preparation of biologically active peptides, in particular to a method for preparing antioxidant active peptides by enzymolyzing crab shells step by step with two enzymes. Background technique [0002] Marine crustaceans are rich in resources. According to statistics from the FAO Fisheries Department of the World Food and Agriculture Organization, the total output of marine crustaceans in the world reached 5.107 million tons in 1996, of which crabs accounted for 23.88% of the total output. Crabs will produce a lot of leftovers after processing, such as crab legs, crab back shells, etc. Due to technical and understanding reasons, most of these shrimp and crab shells are often discarded at will, causing great pollution and waste of resources. However, the processing and utilization of crab shells by domestic production enterprises is still in its infancy, and most of the existing processing and utilization ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34C07K1/14
CPCC07K1/14C07K1/34C12P21/06Y02P20/10
Inventor 刘宇姜维胡世伟李世杰
Owner ZHEJIANG OCEAN UNIV
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