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A magnetic nanoparticle with core-shell structure and its preparation and application

A magnetic nanoparticle, core-shell structure technology, applied in the field of biochemical analysis, can solve the defects of antibody storage, transportation and final use, antibody purification and high cost limit the widespread promotion of immunoassays, unable to provide target protein structure information, sequence and translation Post-modification and other problems to achieve the effect of rapid detection

Active Publication Date: 2020-05-26
SHANGHAI JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, immunoassays have some limitations: due to the specificity of antigen-antibody, the analytical throughput of immunoassays is moderate; immunoassays cannot provide information on the structure, sequence, and post-translational modifications of the target protein; antibody purification and high costs are also Limiting the widespread promotion of immunoassays; due to the fragility of the antibody itself, it leads to defects in storage, transportation and final use; therefore, a high-throughput, high-sensitivity, high-selection method for the extraction and analysis of serum tumor markers needs to be developed urgently

Method used

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  • A magnetic nanoparticle with core-shell structure and its preparation and application
  • A magnetic nanoparticle with core-shell structure and its preparation and application
  • A magnetic nanoparticle with core-shell structure and its preparation and application

Examples

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Embodiment 1

[0042] Example 1: Extraction and mass spectrometry analysis of surface transmembrane glycoprotein CD44 in serum by magnetic nanoparticles with core-shell structure.

[0043] Prepare a serum solution at the level of 2 μg / μL, incubate the core-shell magnetic nanoparticles in the solution for 15 to 30 minutes, and separate the core-shell magnetic nanoparticles capturing the target protein from the solution under the action of magnetic force. Resuspended in normal saline, added trypsin for rapid enzymolysis, and the obtained enzymolyzed product was freeze-dried and stored for electrospray mass spectrometry analysis, which can effectively identify and analyze the protein. Some of the identified CD44 peptides are as follows: image 3shown. It can be seen that using the method provided by the present invention, CD44 protein can be rapidly and effectively separated and extracted, and analyzed by mass spectrometry.

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Abstract

The invention discloses magnetic nano-particles with shell-core structures. The core of each magnetic nano-particle is an iron oxide and the shell of each magnetic nano-particle is silicon dioxide; the surfaces of the magnetic nano-particles are modified by using sodium hyaluronate; the particle size range of the magnetic nano-particles which are modified by the sodium hyaluronate is 350 to 1,000 nm. The invention also discloses a preparation method for the magnetic nano-particles, and application of the magnetic nano-particles in extracting of serum tumour markers and mass spectrometric detection. Based on the magnetic nano-particles which are modified by the sodium hyaluronate, the extracting of the serum tumour markers, such as some cell surface transmembrane glycoprotein, and partial sequence identification can be quickly realized by combining a mass-spectrometric technology, so that high-efficiency and quick detection of a high molecular weight compound is realized. The magnetic nano-particles with the shell-core structures serve as a quick and high-efficiency detection means, and are worth to be popularized and applied.

Description

technical field [0001] The invention relates to the technical field of biochemical analysis, in particular to a magnetic nanoparticle with a core-shell structure and its preparation and application in the extraction and mass spectrometry analysis of serum tumor markers. Background technique [0002] Cell surface transmembrane glycoproteins such as CD44, as a tumor marker, have shown good specificity and sensitivity in clinical accurate diagnosis and treatment. Due to the complexity and low abundance of samples in serum, the detection of tumor markers is very difficult. Much depends on efficient extraction techniques such as immunoassays and antigen-antibody binding. However, immunoassays have some limitations: due to the specificity of antigen-antibody, the analytical throughput of immunoassays is moderate; immunoassays cannot provide information on the structure, sequence, and post-translational modifications of the target protein; antibody purification and high costs are a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J13/02G01N1/40G01N1/34G01N27/62H01F1/11
Inventor 钱昆张汝
Owner SHANGHAI JIAOTONG UNIV
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