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Total bilirubin (oxidization method) detection kit adopting sodium nitrite method

A technology of sodium nitrite and total bilirubin, which is applied in the direction of material analysis by observing the influence on chemical indicators, color/spectral characteristic measurement, and analysis by making materials undergo chemical reactions, etc., which can solve the problem of high reagent prices and difficult promotion , large instrument damage, high requirements, etc., to achieve the effect of high clinical use value, good measurement accuracy, and good stability of reagents

Active Publication Date: 2017-06-09
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The diazonium method has the longest history and is widely used so far. However, the method is affected by many factors when determining direct bilirubin, and the results are quite different. High performance liquid chromatography (HPLC) has requirements for instruments and equipment. high, so it cannot be used as a conventional method; the oxidase method has high sensitivity, but the anti-interference ability is not very strong, mainly because the enzyme has extremely high temperature requirements, and its activity can only play its best role in a certain temperature range, and this kind of The reagent price of the method is extremely high and it is difficult to popularize; the vanadate method is a new oxidation method proposed by Japanese scholars in the 1990s, but this method has great damage to the instrument, and the oxidation of vanadate is higher than that of potassium permanganate , is also not conducive to preserving

Method used

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  • Total bilirubin (oxidization method) detection kit adopting sodium nitrite method
  • Total bilirubin (oxidization method) detection kit adopting sodium nitrite method
  • Total bilirubin (oxidization method) detection kit adopting sodium nitrite method

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Experimental program
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Effect test

Embodiment 1

[0049] In the specific implementation of the present invention, the reagent R1 is:

[0050] Citrate buffer 0.1mol / L (pH2.8)

[0051] Triton X-100 0.3ml / L

[0052] EDTA 1mmol / L

[0053] Hexadecyltrimethylammonium bromide 3mmol / L

[0054] Hydroxyethylidene diphosphonic acid 10mmol / L

[0055] Sodium chloride 9g / L

[0056] Sodium azide 0.05g / L

[0057] Glycerol 10%

[0058] Reagent R2 is:

[0059] Sodium nitrite 4 mmol / L

[0060] Phosphate buffer 10 mmol / L (pH7.0-7.5)

[0061] EDTA-Na 2 0.5g / L

[0062] Sodium carbonate 0.5g / L

[0063] Triton X-100 1ml / L

[0064] Sodium azide 0.05g / L

[0065] Hydroxyethylidene diphosphate 10 mmol / L

[0066] Sodium chloride 0.9g / L

[0067] Mannitol 2g / L

Embodiment 2

[0069] Reagent R1 is:

[0070] Citrate buffer 0.2mol / L (pH2.8)

[0071] Triton X-100 0.5ml / L

[0072] EDTA 1mmol / L

[0073] Cetyltrimethylammonium bromide 5mmol / L

[0074] Hydroxyethylidene diphosphonic acid 10mmol / L

[0075] Sodium chloride 9g / L

[0076] Sodium azide 0.05g / L

[0077] Glycerol 10%

[0078] Reagent R2 is:

[0079] Sodium nitrite 7 mmol / L

[0080] Phosphate buffer 10 mmol / L (pH7.2)

[0081] EDTA-Na 2 0.5g / L

[0082] Sodium carbonate 0.5g / L

[0083] Triton X-100 1ml / L

[0084] Sodium azide 0.05g / L

[0085] Hydroxyethylidene diphosphate 10 mmol / L

[0086] Sodium chloride 0.9g / L

[0087] Mannitol 1.5g / L

Embodiment 3

[0089] Reagent R1 is:

[0090] Citrate buffer 0.2mol / L (pH3.0)

[0091] Triton X-100 0.4ml / L

[0092] EDTA 1mmol / L

[0093] Hydroxyethylidene diphosphonic acid 10mmol / L

[0094] Sodium chloride 9g / L

[0095] Sodium azide 0.05g / L

[0096] Glycerol 15%

[0097] Reagent R2 is:

[0098] Sodium nitrite 10mmol / L

[0099] Phosphate buffer 10 mmol / L (pH7.0)

[0100] EDTA-Na 2 0.5g / L

[0101] Sodium carbonate 0.5g / L

[0102] Triton X-100 1ml / L

[0103] Sodium azide 0.05g / L

[0104] Hydroxyethylidene diphosphate 10mmol / L

[0105] Sodium chloride 0.9g / L

[0106] Mannitol 1.0g / L

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Abstract

The invention provides a total bilirubin (oxidization method) detection kit adopting a sodium nitrite method. Compared with an oxidization method kit which is circulated in the current market, the kit has the advantages that the kit provided by the invention has a relatively wide linear range and good stability and has good clinical interrelation with a contrast reagent. The total bilirubin oxidization method detection kit is supplemented to a certain extent and is also completed. A diazo method detection kit and a vanadate method detection kit, which are circulated in the market at present, have the stability problem with different degrees; the stability of the kit provided by the invention has major breakthrough and a linear range of the kit has the tendency of being widened.

Description

technical field [0001] The invention relates to the technical field of clinical in vitro detection reagents, in particular to detecting and diagnosing the content of total bilirubin in serum by a sodium nitrite method (oxidation method). Background technique [0002] Bilirubin contains two types: free bilirubin and direct bilirubin: direct bilirubin can also be called conjugated bilirubin, that is, free bilirubin in the blood is transported to the liver and is quickly taken up by liver cells. In the cytoplasm of liver cells, it is mainly combined with Y protein and Z protein (mainly Y protein). Then combine with glucuronic acid in the endoplasmic reticulum to generate water-soluble conjugated bilirubin; free bilirubin can also become unconjugated bilirubin, that is, not combined with glucuronic acid, unconjugated bilirubin is mainly heme in Under the catalysis of mononuclear phagocytic system cell microsomal heme oxygenase, the water-soluble biliverdin of linear tetrapyrrol...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N21/78
CPCG01N21/31G01N21/78
Inventor 甘宜梧李建营王绮包兴艳
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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